2004 Cilt 23 Sayı 1-2-3

Permanent URI for this collectionhttps://hdl.handle.net/11452/13142

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Browsing by BUU Author "Nur, Zekariye"

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    Effect of glass wool filtration method on frozen-thawed dog semen
    (Uludağ Üniversitesi, 2004-03-16) Günay, Ülgen; Nur, Zekariye; Soylu, M. Kemal; Uludağ Üniversitesi/Veteriner Fakültesi.
    The aim of this study was to evaluate the effects of the glass wool filtration on motility and morphology of post-thaw quality of dog semen Two German Shepherd Dogs were used as material. Ejaculates were diluted with Tris-citric acid-egg yolk and Sodium citrate-egg yolk extenders at 1:1 ratio including a final 4% glycerol concentration. Semen were frozen in 0.5 ml straws and thawed in a water bath at 37º C for 30 sec. Pasteur pipettes were used as columns. Each column was constructed with 20, 40 and 80 mg glass wool and at 2, 3 and 4 cm heights, respectively. Filtrates were examined before and after filtration for motility, acrosomal, other and total defects. When the filtration methods were compared, the highest improvement percentage in motility was found in column filled with 40 mg glass wool. On the other hand extenders did not have any effect on motility and other morphological defects. But significantly interactions were found between spermatological parameters and filtration techniques. In conclusion, glass wool filtration techniques could be used successfully for filtration of frozen-thawed dog semen.