Browsing by Author "Arat, Sezen"

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Cloning of anatolian grey bull
(Csiro Publishing, 2009) Arat, Sezen; Taş, A.; Bağış, Haydar; Akkoç, Tolga; Çetinkaya, Gaye; Sağırkaya, Hakan; Nak, Yavuz; Nak, Deniz; Uludağ Üniversitesi/Veterinerlik Fakültesi/Doğum & Jinekoloji Anabilim Dalı.; AAH-5494-2021; AAH-8821-2021
Comparing in vitro embryonic development of bovine oocytes cultured in G1.3/G2.3 sequential culture media and CRlaa medium
(Medwell Online, 2009) Çevik, Mesut; Taş, Arzu; Akkoç, Tolga; Bağış, Haydar; Arat, Sezen; Sağırkaya, Hakan; Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı.; AAH-8821-2021; 6602400461
In vitro embryo culture is an important step of in vitro production of bovine embryos. The aim of this study was to compare the in vitro development of bovine embryos in G1.3/G2.3 sequential culture media and CR1aa culture medium. Oocytes were obtained from ovaries of slaughtered cows and matured in TCM-199 medium in humidified air containing 5% CO(2) at 39 degrees C. Matured oocytes were fertilized in vitro using frozen bull sperm prepared with Percoll separation. After fertilization, the presumptive zygotes were denuded from cumulus cells and randomly allotted for 2 treatments: Culture in CR1aa (n = 116) and G1.3/G2.3 (n = 125). The embryo culture was carried out in 50 mu L droplets of the media that were placed in incubator containing 6% CO(2) 5% O(2) and 89% N(2). The embryos were evaluated on days 3, 5 and 7 following in vitro fertilization. The difference of cleavage rates between 2 treatments was not found significant (p>0.05). However, significant difference was observed between 2 treatments at the level of the obtaining rate of morula and blastocyst (p<0.05). The results showed that G1.3/G2.3 sequential media significantly favoured developing of morula and blastocysts in comparison to CR1aa medium (32.0% versus 10.40% versus 18.96 and 2.58%, respectively).
Comparison of the development of mouse embryos manipulated with different biopsy techniques
(TÜBİTAK, 2015-11-08) Taşkın, Ali Cihan; Akkoç, Tolga; Bağış, Haydar; Arat, Sezen; Sağırkaya, Hakan; Uludağ Üniversitesi/Veteriner Fakültesi/Dölerme ve Suni Tohumlama Anabilim Dalı.; AAH-8821-2021; 6602400461
Preimplantation genetic diagnosis is the detection of inherited diseases and the sex of embryos before implantation in the practice of human medicine as well as in veterinary medicine. The introduction of experimental animal embryo biopsy techniques has been a milestone in the developmental process of preimplantation genetic diagnosis techniques. The aim of the present study was to evaluate in vivo and in vitro development of embryos after biopsy in an experimental mouse model and to perform comparisons across different biopsy techniques (blastomere biopsy and trophectoderm biopsy). At the end of the study, no significant difference was observed between the blastomere biopsy group and the control group in terms of in vitro development, embryo quality, and fetal development, whereas embryo quality and in vivo development were negatively affected in the trophectoderm biopsy group (P < 0.05).
Gene expression profiles of vitrified in vitro- and in vivo-derived bovine blastocysts
(Wiley, 2012-09) Aksu, Diğdem Aktopraklıgil; Ağca, Cansu; Aksu, Soner; Bağış, Haydar; Akkoç, Tolga; Çaputçu, Arzu Taş; Arat, Sezen; Taşkın, Ali Cihan; Kızıl, Sedat H.; Karaşahin, Tahir; Akyol, Numan; Satılmış, Muharrem; Ağca, Yüksel; Sağırkaya, Hakan; Üstüner, Burcu; Nur, Zekeriya; Uludağ Üniversitesi/Veteriner Fakültesi.; 0000-0002-1438-221X; AAG-7238-2021; AAH-2635-2021; AAH-8821-2021; 6602400461; 18937724600; 6508060684
Vitrification is becoming a preferred method for pre-implantation embryo cryopreservation. The objective of this study was to determine the differentially expressed genes of in vivo- and in vitro-produced bovine embryos after vitrification. In vitro- (IVF) and in vivo-derived (IVV) bovine blastocysts were identified as follows: in vitro-produced fresh (IVF-F), in vitro-produced vitrified (IVF-V), in vivo-derived fresh (IVV-F), in vivo-derived vitrified (IVV-V). The microarray results showed that 53 genes were differentially regulated between IVF and IVV, and 121 genes were differentially regulated between fresh and vitrified blastocysts (P < 0.05). There were 6, 268, 962, and 17 differentially regulated genes between IVF-F × IVV-F, IVF-V × IVV-V, IVF-F × IVF-V, and IVV-F × IVV-V, respectively (P < 0.05). While gene expression was significantly different between fresh and vitrified IVF blastocysts (P < 0.05), it was similar between fresh and vitrified IVV blastocysts. Significantly up-regulated KEGG pathways included ribosome, oxidative phosphorylation, spliceosome, and oocyte meiosis in the fresh IVF blastocyst samples, while sphingolipid and purine metabolisms were up-regulated in the vitrified IVF blastocyst. The results showed that in vitro bovine blastocyst production protocols used in this study caused no major gene expression differences compared to those of in vivo-produced blastocysts. After vitrification, however, in vitro-produced blastocysts showed major gene expression differences compared to in vivo blastocysts. This study suggests that in vitro-produced embryos are of comparable quality to their in vivo counterparts. Vitrification of in vitro blastocysts, on the other hand, causes significant up-regulation of genes that are involved in stress responses.
Gene expression profiling of in vitro-derived and in vivo-derived bovine blastocysts using microarray analysis
(Current Biology, 2011-09) Aksu, Digdem Aktopraklıgil; Ağca, Cansu; Aksu, Soner; Bağış, Haydar; Akkoç, Tolga; Çaputcu, Arzu Taş; Arat, Sezen; Taşkın, Ali Cihan; Kızıl, Sedat Hamdi; Ağca, Yüksel; Sağırkaya, Hakan; Uludağ Üniversitesi/Veterinerlik Fakültesi/Dölerme ve Suni Tohumlama Bölümü.; AAH-8821-2021
In vitro and in vivo development of embryo following biopsy with different techniques in mouse embryos
(Csiro Publishing, 2013) Taşkın, Ali Cihan; Bağış, Haydar; Akkoç, Tolga; Arat, Sezen; Sağırkaya, Hakan; Uludağ Üniversitesi/Veterinerlik Fakültesi/Dölerme ve Suni Tohumlama Anabilim Dalı.; AAH-8821-2021
Reproductive performance of first cloned Anatolian Grey Cattle produced by frozen cells from National Animal Gene Bank
(Elsevier, 2014-09) Arat, Sezen; Pabuçcuoğlu, Serhat; Alkan, Serhat; Demir, Kamber; Arıcı, Ramazan; Kılıçaslan, Ragıp; Sağırkaya, Hakan; Nak, Yavuz; Alçay, Selim; Üstüner, Burcu; Nak, Deniz; Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Bölümü.; Uludağ Üniversitesi/Veteriner Fakültesi/Doğum ve Jinekoloji Anabilim Dalı.; AAH-8821-2021; AAH-5494-2021
Türkiye yerli koyun ırklarının korunması
(Uludağ Üniversitesi, 2009) Ertuğrul, Mehmet; Dellal, Gürsel; Soysal, İhsan; Akın, Oya; Arat, Sezen; Barıtçı, İlkay; Pehlivan, Erkan; Yılmaz, Orhan; Elmacı, Cengiz; Uludağ Üniversitesi/Ziraat Fakültesi/Zootekni Bölümü.
Yeryüzünün en önemli gen merkezlerinden birinde yer alan Türkiye’nin zengin biyolojik çeşitliliğini korumak ve gerektiğinde kullanmak zorunluluk olarak kabul edilmelidir. Çünkü biyolojik çeşitlilik ekonomik ve genetik zenginliğin bir göstergesi olup, tıp, tarım, gıda güvencesi ve endüstride önemli yararlar sağlamaktadır. Çiftlik hayvanları genetik kaynakları, biyolojik çeşitliliğin önemli bir parçasını oluşturmaktadır. Türkiye'de hayvan genetik kaynakları yeterince değerlendirilememiş, hatta bazıları daha tam olarak tanımlanmadan yok olmuş veya yok olma tehlikesi ile karşı karşıya kalmıştır. Bazı ırklar bilimsel kriterlere göre risk sınırının dışında kabul edilmekle birlikte, azalma hızının çok yüksek olduğu ırklarda mevcut sayının risk sınırına, hatta bu sınırın da altına kısa sürede düşeceği beklenmektedir. Bu durum; ekonomik, sosyal ve çevresel gelişmelerin hayvancılıkta farklı yerlerde çeşitli düzeylerde olmak üzere entansifleşmeyi zorunlu kılması, az girdi ile yetiştirilebilen, buna karşılık düşük verimli olan yerli ırkların yerini hızlı bir şekilde kültür ırkları veya melezlerinin almasının sonucudur. Oysa yerli ırklar yüzyıllardır yetiştirilegeldikleri çevrenin kendine özgü koşullarına çok iyi uyum sağlamış, verimleri düşük olsa bile özgün nitelikleri olan, dayanıklı, kanaatkar, yetersiz çevre koşullarında üreyebilen hayvanlardan oluşur. Yerli ırkların yok olması, taşıdıkları bu ayırıcı özelliklerin de yok olması anlamına gelmektedir. Gelecekte bu özelliklerin hangisine gereksinme duyulacağını şimdiden tahmin etmek güç veya olanaksızdır. Kaldı ki bu gen kaynaklarının bugün saptanmamış olan olası özellikleri de ancak bunların varlıklarını sürdürebilmesi halinde elde tutulabilir. Belirtilen noktalardan hareketle; bildiride Türkiye Yerli Koyun Irklarının korunmasının gerekliliği, korumaya alınacak ırkların belirlenmesi, koruma yöntemleri, Türkiye koyun ırklarının mevcut durumu, bunların korunmasına yönelik çabalar, korumayla ilgili olarak karşılaşılan sorunlar ve alınması gereken önlemler üzerinde durulmaktadır.
Using cell banks as a tool in conservation programmes of native domestic breeds: the production of the first cloned Anatolian Grey cattle
(Csiro Publishing, 2011) Arat, Sezen; Çaputçu, Arzu Taş; Akkoç, Tolga; Pabuccuoğlu, Serhat; Cirit, Umut; Koban, Evren; Bağış, Haydar; Demir, Kamber; Şenünver, Adem; Kılıçaslan, Ragip; Çetinkaya, Gaye; Denizci, Melis; Aslan, Özgür; Sağırkaya, Hakan; Nak, Yavuz; Nak, Deniz; Tuna, Bilginer; Uludağ Üniversitesi/Veterinerlik Fakültesi/Üreme ve Suni Tohumlama Bölümü.; Uludağ Üniversitesi/Veterinerlik Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; AAH-5494-2021; 6602400461; 8615464000; 9280090000
The aim of this study was to clone native Anatolian Grey cattle by using different donor cell types, such as fibroblast, cartilage and granulosa cells cryopreserved in a gene bank and oocytes aspirated from ovaries of Holstein cows as the recipient cytoplasm source. One male calf from fibroblast, three female calves from granulosa cells and one female calf from cartilage cells were born healthy and at normal birthweights. No calves were lost after birth. The results demonstrated that the cloned calves had the same microsatellite alleles at 11 loci as their nuclear donors. However, the mtDNAs of the five Anatolian Grey cloned calves had different haplotypes from their donor cells and mtDNA heteroplasmy could not be detected in any of the clones. The birth of healthy clones suggests that the haplotype difference between the cell and oocyte donor did not affect the pre- or post-implantation development of the bovine nuclear transfer derived embryos in our study. The results showed that well established nuclear transfer protocols could be useful in conserving endangered species. In conclusion, somatic cell banking can be suggested as a tool in conservation programmes of animal genetic resources.