Browsing by Author "AVCI, BERRİN"

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A comparison of the survival and implantation rates of blastocysts that were vitrified on post-fertilization day five, six and seven
(Taylor & Francis Ltd, 2019-07-03) Barış, Ata; AVCI, BERRİN; KUŞPINAR, GÖKTAN; UNCU, GÜRKAN; Gürkan, Uncu; Berrin, Avcı; Işıl, Kasapoğlu; KASAPOĞLU, IŞIL; Göktan, Kuşpınar; Seda, Sarıbal; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Jinekoloji ve Doğum Anabilim Dalı.; ABE-6685-2020
The goal of this retrospective cohort study was to compare survival, implantation, clinical and ongoing pregnancy rates between blastocysts that were vitrified on post-fertilization days 5, 6 and 7. Before vitrification, blastocysts were evaluated in terms of morphology and blastocyst expansion, inner cell mass and trophectoderm quality. They were thawed and transfered in a subsequent artificial cycle. Embryo implantation rates were 39%, 25% and 25% for blastocysts that were vitrified on days 5, 6, and 7, respectively (p = 0.006). Clinical and ongoing pregnancy rates were 19%, 12%, 13% (p = 0.100) and 9%, 7%, 12% (p = 0.99) for days 5, 6 and 7 blastocysts, respectively. Day 5 blastocysts had significantly higher full-collapsing score after assisted-hatching compared to days 6 and 7 blastocysts (p = 0.014). As blastocyst quality increased, implantation and clinical pregnancy rates increased in all groups and both parameters were statistically significantly higher on day 5 blastocysts than on days 6 or 7 (p = 0.001). It was clearly found that good quality blastocysts obtained on day 5 have higher implantation and clinical pregnancy rates than 6th and 7th day cryopreserved embryos. There were no statistically significant differences between the cryopreserved embryos on days 6 and 7 regarding the implantation, clinic and ongoing pregnancy rates.
Amh levels may predict for mullerian anomalies and pregnancy outcomes patients with pcos.
(Elsevier, 2020-09-01) Aslan, Kiper; ASLAN, MÜNİR KİPER; Albayrak, Özge; Bilgiç, Kübra Özlem; BİLGİÇ, KÜBRA ÖZLEM; Kasapoglu, Işıl; KASAPOĞLU, IŞIL; Avci, Berrin; AVCI, BERRİN; Uncu, Gurkan; UNCU, GÜRKAN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; 0000-0002-9277-7735; AAH-9694-2021; AER-7173-2022; AAT-3479-2021
Are all antral follicles the same? Size of antral follicles as a key predictor for response to controlled ovarian stimulation
(Taylor & Francis, 2021-06-21) Kasapoğlu, Işıl; Orhan, Adnan; Aslan, Kiper; Şen, Esra; Kaya, Ayşenur; Avcı, Berrin; Uncu, Gürkan; KASAPOĞLU, IŞIL; ORHAN, ADNAN; ASLAN, MÜNİR KİPER; KAYA, AYŞENUR; AVCI, BERRİN; UNCU, GÜRKAN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; 0000-0002-7558-8166; 0000-0002-9277-7735; AAH-9694-2021; AER-7173-2022; CXJ-7203-2022; V-5292-2019; JWJ-7388-2024; ELU-2357-2022; AAT-3479-2021
The aim of this study was to evaluate whether antral follicle size has any value with respect to antral follicle count (AFC) in predicting ovarian response to controlled ovarian stimulation. Patients who were considered to be normal responders based on their ovarian reserve markers were consecutively recruited. Total AFC was the number of 2-10 mm follicles in both ovaries. Antral follicles were separated into two subgroups according to the size: 2-5 mm and 6-10 mm follicles. Patients were divided into two main groups according to ovarian response to COS. Group 1 (suboptimal response, 4-9 oocytes retrieved, n = 61) and Group 2 (normal responders, >= 10 oocytes retrieved, n = 65). Demographic parameters were comparable between the groups. The number of 2-5 mm follicles was significantly lower in the suboptimal response group (5 [4:7] and 8 [6:10], respectively, p < .001). The ratio of 2-5 mm follicles to total antral follicles was also significantly lower in Group 1 (44.4% and 75%, respectively, p < .001). The ratio of small antral follicles was positively correlated with ovarian response (r = 0.587, p < .001). In conclusion, the ratio of small (2-5 mm) antral follicles could be a more specific predictive marker than AFC for ovarian response.IMPACT STATEMENT What is already known on this subject? Prediction of the ovarian response during ovarian stimulation is commonly based on antral follicle count and anti-mullerian hormone. The ovarian response may be inadequate even in patients with normal antral follicle count and anti-mullerian hormone. What do the results of this study add? A high ratio of small-size (2-5 mm) basal antral follicles is a predictive factor for higher ovarian response to ovarian hyperstimulation. What are the implications of these findings for clinical practice and/or further research? To obtain optimal ovarian response, the antral follicles should be evaluated initially in a more detailed and systematic way by taking their sizes into consideration in addition to their counts. Small antral follicle count rather than whole antral follicle count may be beneficial for optimising the ovarian response. Future studies may determine the cut off values of small antral follicle count for high/poor ovarian response.
Comparison of ICSI outcomes with hypo-osmotic swelling (HOS) reactive sperm and motile sperm on sibling oocytes in patients with severe asthenospermia
(Oxford Univ Press, 2018-07-01) Çakır, C.; Kasapoğlu, Işıl; Sarıbal, Seda; Uncu, Gürkan; Avcı, Berrin; ÇAKIR, CİHAN; KASAPOĞLU, IŞIL; Sarıbal, Seda; UNCU, GÜRKAN; AVCI, BERRİN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları ve Doğum Üreme Biyolojisi; ABE-6685-2020; AAT-3479-2021; JMI-6129-2023; CXJ-7203-2022; FTW-2214-2022
Comparison of semen parameters in the same patients before and after diagnosis of COVID-19
(Wiley, 2023-09-01) Çakır, Cihan; Kuşpınar, Göktan; Kurt, Gizem; Berber, Miray; Aslan, Kiper; Kasapoğlu, Işıl; Uncu, Gürkan; Avcı, Berrin; ÇAKIR, CİHAN; KUŞPINAR, GÖKTAN; Kurt, Gizem; Berber, Miray; ASLAN, MÜNİR KİPER; KASAPOĞLU, IŞIL; UNCU, GÜRKAN; AVCI, BERRİN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; 0000-0002-8332-7353; 0000-0002-9277-7735; AAH-5249-2021; HTQ-5866-2023; DBD-8926-2022; JLV-2420-2023; AER-7173-2022; CXJ-7203-2022; AAT-3479-2021; ELU-2357-2022
Clinical and histopathological evidence suggest that the male reproductive system may be negatively impacted in patients with coronavirus disease (COVID-19). The objective of this study is to investigate the effect of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on semen parameters by comparing semen analyses before and after COVID-19 diagnosis in the same patient. We retrospectively analyzed 342 semen analyses by reviewing medical records. The study included two groups of patients: (i) those who underwent two consecutive semen analyses within 6 months, one before (n = 114) and one after (n = 114) COVID-19 diagnosis, and (ii) a control group (n = 114) that was age-matched and did not receive a diagnosis of COVID-19. The study results indicated a significant decrease in semen volume, total sperm count per ejaculate, progressive motile sperm count, total motile sperm count, and normal sperm morphology after SARS-CoV-2 infection in comparison to their respective values before the infection. Subgroup analyses showed that the duration of COVID-19 diagnosis (short-term vs. long-term) did not impact the changes in semen parameters. However, fever during the COVID-19 process had a negative effect on semen parameters, particularly sperm concentration, unlike in patients without fever. In conclusion, our findings suggest that SARS-CoV-2 infection is associated with a decline in semen quality, which may potentially impact male fertility. Furthermore, it's important to note that the negative effects on semen parameters may persist in the long-term. Our results also indicate that fever during active infection could be a significant risk factor that negatively affects spermatogenesis.
Comparison of survival and implantation rates of blastocysts that were vitrified on post-fertilization day 5, 6 OR 7
(Elsevier, 2015-09-01) Sarıbal, Seda; Uncu, Gürkan; Ata, Barış; Avcı, Berrin; Sarıbal, Seda; UNCU, GÜRKAN; AVCI, BERRİN; Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Bölümü; Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları ve Doğum; ABE-6685-2020; AAT-3479-2021; FTW-2214-2022
Does adjuvant usage in the luteal phase improve live birth rates of icsi - et patients with previous implantation failure?
(Oxford Univ Press, 2022-07-01) UNCU, GÜRKAN; Uncu, Gürkan; Kasapoğlu, I.; KASAPOĞLU, IŞIL; ASLAN, MÜNİR KİPER; Çınar, C.; Kuşpınar, G.; KUŞPINAR, GÖKTAN; Avcı, B.; AVCI, BERRİN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; 0000-0002-9277-7735; HTQ-5866-2023; AER-7173-2022
Does the recurrence of ovarian endometrioma affect the pregnancy rates in IVF?
(Elsevier, 2015-09-01) Aslan, Kiper; Kasapoğlu, Işıl; Avcı, Berrin; Ata, Barış; Uncu, Gürkan; ASLAN, MÜNİR KİPER; KASAPOĞLU, IŞIL; AVCI, BERRİN; Ata, Barış; UNCU, GÜRKAN; Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları; Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Bölümü; Uludağ Üniversitesi/Tıp Fakültesi/Endokrinolojisi Bölümü; 0000-0002-9277-7735; 0000-0003-1106-3747; ABE-6685-2020; AAH-9694-2021; AER-7173-2022; C-8049-2013; AAT-3479-2021; CXJ-7203-2022
Effect of embryo morphology on maternal serum β-hCG level in pregnancies resulting from a fresh single cleavage embryo and a fresh single blastocyst
(Oxford Univ Press, 2018-07-01) Kuşpınar, Göktan; Kasapoğlu, Işıl; Sarıbal, Seda; Uncu, Gürkan; Avcı, Berrin; KUŞPINAR, GÖKTAN; KASAPOĞLU, IŞIL; Sarıbal, Seda; UNCU, GÜRKAN; AVCI, BERRİN; Bursa Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü/Kadın Hastalıkları ve Doğum Anabilim Dalı; 0000-0002-0338-8368; AAH-5119-2021; HTQ-5866-2023; AAT-3479-2021; ABE-6685-2020; CXJ-7203-2022; FTW-2214-2022
Evaluation of endometrioma pathophysiology and related ovarian damage by PTEN / AKT apoptosis signaling pathway
(Elsevier Science, 2020-09-01) Şen, Furkan; Aslan, Kiper; Kuşpınar, Göktan; Çakır, Cihan; Kasapoglu, Işıl; Avcı, Berrin; Uncu, Gürkan; ŞEN, HAMZA FURKAN; ASLAN, MÜNİR KİPER; KUŞPINAR, GÖKTAN; ÇAKIR, CİHAN; KASAPOĞLU, IŞIL; AVCI, BERRİN; UNCU, GÜRKAN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi.; 0000-0002-9277-7735; 0000-0002-8332-7353; AAH-5119-2021; AAT-3479-2021; HTQ-5866-2023; AER-7173-2022; AAH-9694-2021; AAH-5249-2021; KEU-2073-2024; CXJ-7203-2022; ELU-2357-2022
Fertilisation and early embryonic development of immature and rescue in vitro-matured sibling oocytes
(Taylor & Francis, 2020-01-16) Avcı, Berrin; Kasapoğlu, Işıl; Çakir, Cihan; Özbay, Aysun; Ata, Barış; Uncu, Gürkan; AVCI, BERRİN; KASAPOĞLU, IŞIL; ÇAKIR, CİHAN; Özbay, Aysun; UNCU, GÜRKAN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; 0000-0002-8332-7353; 0000-0001-8135-5468; AAT-3479-2021; GQR-0770-2022; AAH-5249-2021; CXJ-7203-2022 ; ABE-6685-2020
The objective of this study was to assess the effect of rescue in vitro maturation and immediate intracytoplasmic sperm injection (ICSI) application on fertilisation success and early embryonic development of metaphase I (MI) oocytes. This was a retrospective cohort study including 2425 sibling oocytes in 259 ICSI cycles. ICSI was performed on 104 GV (germinal vesicle) oocytes which had reached the metaphase II (MII) stage (Group 1) and 231 MI oocytes which had reached the MII stage (Group 2) following IVM (in vitro maturation). Immediate ICSI was applied following oocyte aspiration on 292 MI stage (Group 3) and 1798 MII stage oocytes (Group 4). Normal fertilisation rates in Groups 1, 2, 3 and 4 were 51.9%, 39%, 30.1% and 59.5%, respectively. The rates of blastocyst development per oocyte and per zygote were calculated as 3.8%, 3.0%, 6.8%, 14.1% and 7.4%, 7.7%, 22.7%, 23.6% for Groups 1, 2, 3 and 4, respectively. The blastocyst development rate was significantly higher in the MI-ICSI group compared with other immature oocytes. Even though performing ICSI on the oocytes at the MI stage on the day of oocyte aspiration resulted in lower fertilisation rates, it was associated with significantly higher rates of blastocyst development.
Low molecular weight heparin-aspirin-prednisolone combination does not increase the live birth rate in recurrent implantation failure: A retrospective cohort study
(Springer Heidelberg, 2023-05-30) Aslan, Kiper; Kasapoğlu, Işıl; Çınar, Ceren; Çakır, Cihan; Avcı, Berrin; Uncu, Gürkan; ASLAN, MÜNİR KİPER; KASAPOĞLU, IŞIL; ÇINAR, CEREN; ÇAKIR, CİHAN; AVCI, BERRİN; UNCU, GÜRKAN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; 0000-0002-9277-7735; 0000-0002-8332-7353; AER-7173-2022; CXJ-7203-2022; IUD-1217-2023; AAH-5249-2021; ELU-2357-2022; AAT-3479-2021
This study investigates the triple combination of adjuvants (low molecular weight heparin (LMWH)-aspirin-prednisolone) whether it improves the live birth rates of IVF&ICSI patients with previous implantation failure. This retrospective study included 1095 patients with >2 failed either fresh or frozen single embryo transfer cycles between 2014 Jan and 2021 Jan. Patients were divided into two subgroups. Group A consisted of patients with only vaginal progesterone for luteal phase support. Group B consisted of patients with triple (daily subcutaneous LMWH, daily 150 mg aspirin, and daily 16 mg prednisolone) luteal phase supplementation to vaginal progesterone. Demographic parameters, cycle characteristics, embryology, and pregnancy outcomes were compared, and the study's primary outcome was the live birth rate. Demographic parameters were similar between the groups. Positive b-hCG, miscarriage, and live birth rates were similar between groups as Group A vs. Group B, positive b-hCG 30.8% (190/617) vs. 35.4% (169/478), miscarriage rates 4.4% (27/617) vs. 6.7% (32/478), and live birth rates 20.4% (126/617) vs. 23.8% (114/478), respectively. When patients were stratified according to previous failures, live birth rates were still similar. Pregnancy outcomes were significantly improved in only patients with diminished ovarian reserve (Group A vs. Group B, positive b-hCG 24.2% vs. 34.3%, live birth rate 12.1% vs. 21.9%, p < 0.01). Whether the embryo transfer was fresh or frozen-thawed did not affect the results. A combined supplementation of LMWH, aspirin, and prednisolone in the luteal phase does not improve live birth rates of IVF&ICSI patients with previous implantation failure except potentially for patients with diminished ovarian reserve.
Luteal oestradiol for patients with serum oestradiol levels lower than expected per oocyte
(Taylor, 2021-03-15) Kasapoğlu, Işıl; Düzok, Nergis; Şen, Esra; Çakır, Cihan; Avcı, Berrin; Uncu, Gürkan; KASAPOĞLU, IŞIL; DÜZOK, NERGİS; Şen, Esra; ÇAKIR, CİHAN; AVCI, BERRİN; UNCU, GÜRKAN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı; 0000-0002-8332-7353; AAT-3479-2021; AAH-5249-2021; CXJ-7203-2022; JLC-5688-2023; FVL-9509-2022; ELU-2357-2022
Although the efficiency of progesterone in providing luteal phase support has been established, the role of oestradiol supplementation during the luteal phase remains controversial. We evaluated pregnancy outcomes of patients who had a ratio of serum E2 levels on the hCG day to the number of oocytes retrieved (oestradiol/oocyte ratio - EOR) levels of <100 pg/ml by supporting them with additional oestradiol during the luteal phase. In total, 150 patients with an EOR < 100 pg/ml of oestradiol undergoing antagonist intracytoplasmic sperm injection (ICSI) cycles were randomly assigned into two groups to receive either oral oestradiol (4 mg/d) plus vaginal progesterone (90 mg, 2 x 1/day) (group 1) or vaginal progesterone (90 mg, 2 x 1/d) alone (group 2). Implantation rate following transfer of a single embryo did not differ between the two groups (group 1 = 33.3%; group 2 = 34.9%; p = 0.85). Similarly, both groups gave comparable clinical pregnancy rates per embryo transfer with 31.7% in group 1 compared with 28.6% in group 2 (p = 0.69). In conclusion the study suggests that the addition of 4 mg oral E2 to progesterone does not increase the probability of pregnancy.
Scheduling gnrh antagonist cycles by a short course of oral estradiol administration during early follicular phase: A comparative study with non-scheduled cycles
(Taylor & Francis Ltd, 2015-06-01) Aslan, Kiper; ASLAN, MÜNİR KİPER; UNCU, GÜRKAN; Avci, Berrin; AVCI, BERRİN; Uncu, Gürkan; Saribal, Seda; Ata, Barış; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları Anabilim Dalı.; 0000-0002-9277-7735; 0000-0003-1106-3747; C-8049-2013; AAT-3479-2021; AER-7173-2022; AAH-9694-2021; ABE-6685-2020
This hypothesis generating study investigated whether GnRH antagonist cycles can be scheduled by a short course of oral estradiol administration during the follicular phase without impairing treatment outcome. Thirty-five women who underwent follicular phase estrogen scheduling (ES) of GnRH antagonist cycles were retrospectively matched for age and number of prior failed cycles with 35 women who underwent unscheduled GnRH antagonist cycles. ES group was given 6 mg/day estradiol orally from cycle day 2 until (including) one day before the scheduled start of stimulation. Gonadotropins were started on cycle days 2-3 in the control group. Flexible GnRH antagonist protocol was employed in both groups. ES group received estradiol for a median of 5 days. Total gonadotropin consumption was similar but one more GnRH antagonist injection was required in the ES group. Endometrial thickness on the day of hCG injection was increased in the ES group (12 versus 10 mm, p<0.01). Number of oocytes, metaphase II oocytes and transferred embryos were similar. Embryo implantation rates were 44.8% versus 34.4% (p=0.3), and clinical pregnancy rates were 48.6% versus 37.1%, (p=0.33) in the ES and control groups, respectively. All women in the ES group had oocyte retrieval and embryo transfer within the desired period.
The effects of endometriosis on oocyte morphology and embryo development
(Oxford Univ Press, 2022-07-01) Avcı, Berrin; Kuspınar, Göktan; KUŞPINAR, GÖKTAN; UNCU, GÜRKAN; Çakır, Cihan; Aslan, Kiper; ASLAN, MÜNİR KİPER; Uncu, Gürkan; Kasapoğlu, Işıl; AVCI, BERRİN; ÇAKIR, CİHAN; KASAPOĞLU, IŞIL; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; 0000-0002-8332-7353; 0000-0002-9277-7735; HTQ-5866-2023; AER-7173-2022; AAH-5249-2021
The efficacy and molecular mechanism of dehydroepiandrosterone in diminished ovarian reserve
(Oxford Univ Press, 2020-07-01) Avcı, Berrin; AVCI, BERRİN; Çakır, C.; Kuşpınar, Göktan; KUŞPINAR, GÖKTAN; Işıklar, S.; Aslan, Kiper; ASLAN, MÜNİR KİPER; Kasapoğlu, I.; Uncu, Gürkan; UNCU, GÜRKAN; Bursa Uludağ Üniversitesi/Tıp Fakültesi; 0000-0002-9277-7735; HTQ-5866-2023; AAH-9694-2021; AAH-5119-2021; ABE-6685-2020; AER-7173-2022; AAT-3479-2021
The kisspeptin and kisspeptin receptor in follicular microenvironment: Is that really necessary for oocyte maturation and fertilisation?
(Taylor & Francis Inc, 2022-08-19) Kuşpinar, Göktan; KUŞPINAR, GÖKTAN; Çakır, Cihan; ORAL, HALUK BARBAROS; ÇAKIR, CİHAN; KASAPOĞLU, IŞIL; Sarıbal, Seda; BUDAK, FERAH; Budak, Ferah; Avcı, Berrin; Uncu, Gürkan; UNCU, GÜRKAN; AVCI, BERRİN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/İmmunoloji Anabilim Dalı.; 0000-0002-8332-7353; 0000-0001-7625-9148; IZP-9398-2023; AAH-5249-2021; F-4657-2014; HTQ-5866-2023
The aim of this study was to determine whether Kisspeptin and Kisspeptin receptor in the follicular microenvironment is necessary for human oocyte maturation and fertilisation. The cumulus cell (CC) and follicle fluids (FF) obtained from the first aspirated follicles (n = 52) from 32 patients were divided into three groups considering nuclear maturation and fertilisation results of oocytes: (1) Metaphase I or germinal vesicle stage oocytes (incomplete nuclear maturation, n = 10), (2) unfertilised metaphase II oocytes (incomplete cytoplasmic maturation, n = 16), and (3) fertilised metaphase II oocytes (completed nuclear-cytoplasmic maturation, n = 26). The gene expression levels were assessed by RT-PCR. The levels of Kisspeptin (KISS1) and Kisspeptin receptor (KISS1R) were measured by ELISA. There were no significant efficacy KISS1 and KISS1R gene expressions in cumulus cells in terms of oocyte nuclear maturation stage (Group 1, vs Group 2 + Group 3) (respectively p = .49; p = .45). In terms of the cytoplasmic maturation stage (Group 2, vs Group 3); KISS1 and KISS1R expressions in CCs were comparable (respectively p = .07; p = .08). In FFs, KISS1 and KISS1R concentrations were similar between all groups (respectively p = .86; p = .26). In conclusion, the relative KISS1 and KISS1R expressions in CC and also KISS1 and KISS1R level of FF were independent of oocytes nuclear and/or cytoplasmic maturation. Impact statement What is already known on this subject? It has been demonstrated that Kisspeptin is an essential regulator of reproductive function and plays a key role in the modulation of GnRH secretion and gonadotropin release. Still, no information is available about the link between gene expression or concentration in the follicular microenvironment and oocyte development. What do the results of this study add? The study has shown that the relative Kisspeptin (KISS1) and Kisspeptin receptor (KISS1R) and expressions in cumulus cell (CC) and also KISS1 and KISS1R levels of follicle fluids (FF) were independent of oocytes nuclear and/or cytoplasmic maturation. What are the implications of these findings for clinical practice and/or further research? Based on the findings, it is difficult to establish a concept that kisspeptin can directly induce oocyte maturation. Nevertheless, to confirm these findings, further studies with a larger sample size are needed.
What is the effect of embryo morphology on serum β-hCG levels?
(Elsevier, 2019-02-01) Kuşpınar, Göktan; Kasapoğlu, Işıl; Çakır, Cihan; Ata, Barış; Uncu, Gürkan; Avcı, Berrin; KUŞPINAR, GÖKTAN; KASAPOĞLU, IŞIL; ÇAKIR, CİHAN; UNCU, GÜRKAN; AVCI, BERRİN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; 0000-0002-8332-7353; 0000-0002-0338-8368; HTQ-5866-2023; ABE-6685-2020; AAT-3479-2021; AAH-5249-2021; CXJ-7203-2022 ; AAH-5119-2021
Objective: To determine the effect of embryonic factors on serum beta human chorionic gonadotropin (beta-hCG) levels in pregnancy and live birth resulting after a single fresh cleavage embryo and blastocyst transfer.Study design: This was a retrospective cohort study conducted at a tertiary care hospital. All fresh single embryo transfers (sETs) between September 2011 and December 2016 were included. The correlation analysis was performed to determine the association of embryo morphological parameters on mean serum beta-hCG levels on day 12 after the transfer of a fresh single cleavage embryo and a fresh single blastocyst embryo.Results: Out of a total of 455 fresh sETs, 60 positive beta-hCG results after the transfer of a single fresh cleavage-stage embryo and 82 after the transfer of a single fresh blastocyst. The mean beta-hCG level resulting from a single fresh blastocyst ET was 371.7 +/- 52.7 IU/L, which was similar to the mean beta-hCG level resulting from a cleavage ET (314.5 +/- 36.9 IU/L) (p=.70). Interestingly, serum beta-hCG levels resulting from a single fresh blastocyst ET showed a correlation with day 5 blastocoele expansion, trophectoderm cell number and blastocyst quality score in ongoing pregnancy (r = .33, p = .02; r = .29, p = .04; and r = .31, p = .03, respectively). Moreover, day 5 blastocoele expansion and blastocyst quality score showed a correlation with the serum beta-hCG levels resulting from a single fresh blastocyst ET in live birth (r = .36, p = .02; r = .31, p = .04, respectively).Conclusion: Our study suggests that serum beta-hCG levels resulting from a single fresh blastocyst ET showed a correlation with day 5 blastocoele expansion and blastocyst quality score in both ongoing pregnancy and live birth. (C) 2018 Elsevier B.V. All rights reserved.
Which is more predictive ovarian reserve marker when there is discordance between anti - mullerian hormone and antral follicle count in patients with diminished ovarian reserve, amh or afc?.
(Elsevier, 2020-09-01) Aslan, Kiper; ASLAN, MÜNİR KİPER; Kasapoglu, Isil; KASAPOĞLU, IŞIL; Koç, Meltem; KOÇ ÇAKAR, MELTEM; Kuşpınar, Göktan; KUŞPINAR, GÖKTAN; Avcı, Berrin; AVCI, BERRİN; Uncu, Gürkan; UNCU, GÜRKAN; Bursa Uludağ Üniversitesi/Tıp Fakültesi; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; 0000-0002-9277-7735; 0000-0001-6456-8779; HTQ-5866-2023; AAH-9694-2021; AAH-5119-2021; AER-7173-2022; AAT-3479-2021
Amer Soc Reprod Med