Broyler yemlerine zink basitrasin, probiyotik ve mannanoligosakkaritleri katkısının ince barsak morfolojisi üzerine etkileri
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Date
1999-06-16
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Uludağ Üniversitesi
Abstract
Bu çalışma, broyler yemlerine katılan zink basitrasin, probiyotik ve mannanoligosakkaritlerinin ince barsak morfolojisi üzerine etkilerinin belirlenmesi amacıyla yapılmıştır. Denemede toplam 40 adet günlük yaşta Avian Farms broyler erkek civciv kullanılmış ve civcivler Kontrol, Mannanoligosakkaritleri (MO), Zink Basitrasin (ZB) ve Probiyotik (P) olarak dört gruba ayrılmışlardır. Kontrol grubundan farklı olarak, MO grubunun yemlerine 1g/kg yem dozunda mannanoligosakkaritleri (Bio-Mos ), ZB grubunun yemlerine ise 50mg/kg yem dozunda zink basitrasin katkısı yapılmıştır (AlbacR). İlk üç hafta, P grubunun yemlerine probiyotik (Primalac: Lactobacillus acidophilus 4.52x108 cfu g'', Lactobacillus casei 1.32x10 cfu gi'. Streptococcus faecium 2.8x100 cfu gl, Bifidobacterium thermophilus 1.36x100 cfu go') katkısı yapılmazken içme sularına 0.114 g/L dozunda probiyotik katılmıştır. Dördüncü haftadan sonra probiyotik katkısı, P grubunun içme sularından kaldırılarak geliştirme yemlerine 1 g/kg yem dozunda yapılmıştır. Deneme 39. günde bitirilmiştir. Kesimden sonra alınan ince barsak örnekleri mikroskobik olarak incelenmiştir. Villus uzunlukları, villus yükseklikleri, villus genişlikleri ve kadeh hücresi sayıları belirlenmiş ve kript derinlikleri hesaplanmıştır. Kontrol grubu ile karşılaştırıldığında; ZB grubunda ileumun kript derinliğinin azaldığı, MO grubunda villus yüksekliğinin ileum ve jejunumda, villus genişliğinin ise sadece jejunumda arttığı saptanmıştır. P grubunda, villus yüksekliğinin ileumda arttığı, villus genişliğinin jejunum ve ileumda, kript derinliğinin ise sadece ileumda azaldığı belirlenmiştir. Villus uzunluğu ve kadeh hücresi sayısındaki farklılıklar istatistiki olarak önemsiz bulunmuştur.
This study was performed to determine the effects of zinc bacitracin, probiotic and mannanoligosaccharide supplemented into the broiler feeds on small intestine morphology. In the experiment, a total of 40 one day old Avian Farms male broiler chicks were used, and they were assigned in four goups as Control, Mannanoligosaccharide (MO), Zinc Bacitracin (ZB) and Probiotic (P). Separately from Control, feeds prepared for MO and ZB groups were supplemented with 1g/kg feed mannanoligosaccharide (Bio-Mos) and 50mg/kg feed zinc bacitracin respectively. In the first three weeks, drinking water of P group contained 0.114g/per liter probiotic (Primalac: Lactobacillus acidophilus 4.52x100 cfu g*, Lactobacillus casei 1.32x108 cfu g', Streptococcus faecium 2.8x10' cfu g', Bifidobacterium thermophilus 1.36x10 cfu g') while starter feed was not supplemented with probiotic. But, from 4th weeks to the end of the trial, grower feed of P group was supplemented with probiotic (Primalac Ig/kg feed) while drinking water did not contain probiotic. The experiment was terminated at 39th day. Small intestine samples taken after the slaughtering, were examined microscopically. Villus lengths, heights, widths and goblet cell numbers were measured, and the crypt depths were calculated. When compared to the Control; crypt depth of the ileum reduced in ZB group, ileal and jejunal villus height and jejunal villus width only increased in MO group. In P group, villus height increased at the ileum, villus width decreased at the jejunum and ileum and also crypt depth reduced only in ileum. The differences at the villus length and goblet cell numbers were not valuable statistically.
This study was performed to determine the effects of zinc bacitracin, probiotic and mannanoligosaccharide supplemented into the broiler feeds on small intestine morphology. In the experiment, a total of 40 one day old Avian Farms male broiler chicks were used, and they were assigned in four goups as Control, Mannanoligosaccharide (MO), Zinc Bacitracin (ZB) and Probiotic (P). Separately from Control, feeds prepared for MO and ZB groups were supplemented with 1g/kg feed mannanoligosaccharide (Bio-Mos) and 50mg/kg feed zinc bacitracin respectively. In the first three weeks, drinking water of P group contained 0.114g/per liter probiotic (Primalac: Lactobacillus acidophilus 4.52x100 cfu g*, Lactobacillus casei 1.32x108 cfu g', Streptococcus faecium 2.8x10' cfu g', Bifidobacterium thermophilus 1.36x10 cfu g') while starter feed was not supplemented with probiotic. But, from 4th weeks to the end of the trial, grower feed of P group was supplemented with probiotic (Primalac Ig/kg feed) while drinking water did not contain probiotic. The experiment was terminated at 39th day. Small intestine samples taken after the slaughtering, were examined microscopically. Villus lengths, heights, widths and goblet cell numbers were measured, and the crypt depths were calculated. When compared to the Control; crypt depth of the ileum reduced in ZB group, ileal and jejunal villus height and jejunal villus width only increased in MO group. In P group, villus height increased at the ileum, villus width decreased at the jejunum and ileum and also crypt depth reduced only in ileum. The differences at the villus length and goblet cell numbers were not valuable statistically.
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Keywords
Mannanoligosakkaritleri, Broyler, Zink basitrasin, Probiyotik, Barsak, Morfoloji
Citation
Sönmez, G. ve Eren, M. (1999). "Broyler yemlerine zink basitrasin, probiyotik ve mannanoligosakkaritleri katkısının ince barsak morfolojisi üzerine etkileri". Uludağ Üniversitesi Veteriner Fakültesi Dergisi, 18(3), 125-138.