Determination of allelic deletion of multiple endocrine neoplasm type 1 (MEN1) gene in acute myeloid leukemia (AML) by application of FISH-TSA technique
Date
2002
Authors
Acar, Hasan
Kaynak, Murat
Uçar, Fahri
Journal Title
Journal ISSN
Volume Title
Publisher
Wiley Liss
Abstract
We have used the single and dual color fluorescence in situ hybridization (FISH) technique combined with a new detection system, tyramide signal amplification (TSA), by using the multiple endocrine neoplasm type I (MEN1) gene and chromosome I I specific alpha satellite DNA probes for the study of the allelic deletion of the MEN1 gene. The MEN1 gene is a new tumor supressor gene and has been recently cloned on chromosome 11q13. FISH combined with the TSA detection system was performed on bone marrow interphase nuclei of 22 patients with acute myeloid leukemia (AML). The FISH-TSA analysis revealed the mono allelic deletion of the MEN1 gene in 4 out of 22 patients (18.18%), 2 of 9 AML-M2 patients (22.2%), 1 of 6 AML-M4 patients (16.6%), and I of 4 AML-M5 patients (25.0%). Our study indicates that allelic deletion of the MEN1 gene is not a major cause or a primary event in tumorigenesis of AML, although the long arm (q13 region) of chromosome 11 involves a chromosomal rearrangement in AML.
Description
Keywords
Allelic deletion, AML, FISH, MEN1 gene, TSA, In-situ hybridization, Chromosome 11q13, Mll gene, Heterozygosity, Amplification, Carcinomas, Mutaions, Region, Head, Oncology, Genetics & heredity, Toxicology
Citation
Acar, H. vd. (2002). "Determination of allelic deletion of multiple endocrine neoplasm type 1 (MEN1) gene in acute myeloid leukemia (AML) by application of FISH-TSA technique". Teratogenesis Carcinogenesis and Mutagenesis, 22(5), 369-375.