Eritrosit membranında fibrinolitik aktivitenin araştılması
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Date
1983
Authors
Ulutin, Orhan N.
Journal Title
Journal ISSN
Volume Title
Publisher
Uludağ Üniversitesi
Abstract
Çalışmamızda pıhtılaşma mekanizmasına katkısı olan eritrositlerin, fibrinolitik aktivitesinin araştırılması amaçlandı. Eritrositler distile su ile patlatılarak , distile su ile iyice yıkandı. Çöken eritrosit membranları owren tamponu ile süspansiyon şekline getirildi. Eritrosit membranları 30 saniye aralıkla toplam 60 saniye buz içinde sonik osilasyona tabi tutularak membranları parçalandı. Membran parçacıkları owren tamponu ile çözüldü. Membran fibrinolitik aktiviteleri E.E.Z. (Euglobulin Erime Zamanı) ve fibrin plak yöntemiyle ölçüldü. Her iki yöntemle de anlamlı bir aktivite görüldü.
In this study, fibrinolytic activity of erythrocytes which have on influence on the coagulation mechanism, has been investigated. Erythrocytes were washed thoroughly and burst in distilled water. The solution was prepared by adding owren buffer on the precipitated erythrocyte membranes. Erytrocyte membranes are operated by sonic oscillation in ice for 60 seconds each cell for 30 seconds consecutively. The membrane particules were solved in owren buffer. Membrane fibrinolytic activity were used to measure by E.L.T. (Euglobulin Lysis Time) and fibrin plate methods. With both method reasonable activities were seen.
In this study, fibrinolytic activity of erythrocytes which have on influence on the coagulation mechanism, has been investigated. Erythrocytes were washed thoroughly and burst in distilled water. The solution was prepared by adding owren buffer on the precipitated erythrocyte membranes. Erytrocyte membranes are operated by sonic oscillation in ice for 60 seconds each cell for 30 seconds consecutively. The membrane particules were solved in owren buffer. Membrane fibrinolytic activity were used to measure by E.L.T. (Euglobulin Lysis Time) and fibrin plate methods. With both method reasonable activities were seen.
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Keywords
Eritrosit membranı, Fibrinolitik aktivite, Erythrocytes membrane, Fibrinolytic activity
Citation
Özlük, K. ve Ulutin, O. N. (1983). "Eritrosit membranında fibrinolitik aktivitenin araştılması". Uludağ Üniversitesi Tıp Fakültesi Dergisi, 10(2), 171-178.