Simultaneous detection of six different groups of antimicrobial drugs in porcine oral fluids using a biochip array-based immunoassay

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dc.contributor.authorRumbeiha, Wilson K.
dc.contributor.authorEnsley, Steve
dc.contributor.authorOlsen, Chris
dc.contributor.authorSchrunk, Dwayne E.
dc.contributor.buuauthorOruç, Hasan Hüseyin
dc.contributor.departmentUludağ Üniversitesi/Veterinerlik Fakültesi/Farmakoloji ve Toksikoloji Anabilim Dalı.tr_TR
dc.contributor.orcid0000-0002-5399-2395tr_TR
dc.contributor.researcheridAAI-2212-2021tr_TR
dc.contributor.scopusid55944769400tr_TR
dc.date.accessioned2024-04-01T07:42:54Z
dc.date.available2024-04-01T07:42:54Z
dc.date.issued2013
dc.description.abstractThe objectives of this study were 1) to determine whether a biochip array-based immunoassay could be used to detect 6 different group antimicrobials in porcine oral fluids collected under experimental conditions; 2) to determine the feasibility of antimicrobial detection in clean versus dirty oral fluid samples; and 3) to determine if the assay could be used to detect chlortetracycline in oral fluids of swine ingesting a routine diet containing chlortetracycline. Biochip array-based immunoassay is currently used for simultaneous detection and quantitation of different group six antimicrobials in milk, urine, meat, honey, and feed. The assay had not been investigated for potential use in oral fluids. Following evaluation of different extraction procedures, a centrifugation method was chosen. Results showed that of the six target drugs, norfloxacin, ceftiofur, florfenicol, streptomycin, tylosin and tetracycline could be detected in both clean and dirty oral fluid samples. However, tetracycline was not well recovered in dirty samples. Chlortetracycline from tetracycline group was detected in all oral fluid samples collected from the field at concentrations ranging from 176.0-698.6 ppb. In conclusion, this assay can detect all target antibacterials from different groups in clean and dirty oral fluid samples with the exception of tetracycline which was not well-recovered from in dirty samples. en_US
dc.description.sponsorshipMinistry of National Education - Turkeytr_TR
dc.identifier.citationOruç, H. H. (2013). “Simultaneous detection of six different groups of antimicrobial drugs in porcine oral fluids using a biochip array-based immunoassay”. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 19(3), 407-412.tr_TR
dc.identifier.endpage412tr_TR
dc.identifier.issn1300-6045
dc.identifier.issue3tr_TR
dc.identifier.scopus2-s2.0-84875039200tr_TR
dc.identifier.startpage407tr_TR
dc.identifier.urihttps://doi.org/10.9775/kvfd.2012.7905
dc.identifier.urihttps://hdl.handle.net/11452/40881
dc.identifier.volume19tr_TR
dc.identifier.wos000321750600007tr_TR
dc.indexed.scopusScopus en_US
dc.indexed.trdizinTRDizin tr_TR
dc.indexed.wosSCIE en_US
dc.language.isoen en_US
dc.publisherKafkas Üniversitesi tr_TR
dc.relation.collaborationYurt dışıtr_TR
dc.relation.journalKafkas Üniversitesi Veteriner Fakültesi Dergisi tr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/openAccess en_US
dc.subjectVeterinary sciences en_US
dc.subjectAntimicrobials en_US
dc.subjectBiochip array-based immunoassay en_US
dc.subjectOral fluid en_US
dc.subjectSwine en_US
dc.subjectSaliva en_US
dc.subject.scopusMouth; Classical Swine Fever; Swine Diseases en_US
dc.subject.wosVeterinary sciences en_US
dc.titleSimultaneous detection of six different groups of antimicrobial drugs in porcine oral fluids using a biochip array-based immunoassay en_US
dc.typeArticleen_US

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