Strain improvement by UV mutagenesis for protease overproduction from bacillus subtilis E6-5 and nutritional optimization

Simple item page
dc.contributor.buuauthorDemirkan, Elif
dc.contributor.buuauthorSevgi, Tuba
dc.contributor.buuauthorGokoz, Meltem
dc.contributor.buuauthorGuler, Baran Enes
dc.contributor.buuauthorZeren, Behice
dc.contributor.buuauthorOzalpar, Busra
dc.contributor.buuauthorAbdou, Maoulida
dc.contributor.departmentBursa Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Biyoloji Bölümü.tr_TR
dc.date.accessioned2021-07-14T06:25:38Z
dc.date.available2021-07-14T06:25:38Z
dc.date.issued2018-08-01
dc.description.abstractThe purpose of the present study was to enhance the protease production of the parental type Bacillus subtilis E6-5 by UV irradiation. The parental type was subjected to UV irradiation at different distances (5-15 cm) for different time intervals ( 1-120 min). After each treatment, total 400 mutants were obtained. The mutants were screened on skim milk agar plates for the selection of best proteolytic mutant. Among mutants, the mutant MET39, which obtained at 15 cm distance and irradiation time 5 min of exposure, was selected as best mutant produced 1.5 fold more enzyme over the parent strain. The effects of nutritional factors (various carbon, nitrogen sources and metal ions) on the protease production from MET39 mutant strain were studied. The best carbon source was found as glycerol. Among the inorganic nitrogen sources, the highest enzyme production was obtained in the presence of tryptone. The metal ions did not indicate significant effect on enzyme production. In order to enhance the yield, new modified medium was obtained by combining the best carbon and nitrogen sources. In this medium, enzyme yield was enhanced 88% compared to basal medium. MET39 mutant strain might have a great potential for protease production at industrial scale.en_US
dc.identifier.citationDemirkan, E. vd. (2018). ''Strain improvement by UV mutagenesis for protease overproduction from bacillus subtilis E6-5 and nutritional optimization''. Journal of Biological and Environmental Sciences, 12(35), 69-77.tr_TR
dc.identifier.endpage77tr_TR
dc.identifier.issn1307-9530
dc.identifier.issn1308-2019
dc.identifier.issue35tr_TR
dc.identifier.startpage69tr_TR
dc.identifier.urihttp://jbes.uludag.edu.tr/PDFDOSYALAR/35/mak05.pdf
dc.identifier.urihttp://hdl.handle.net/11452/21258
dc.identifier.volume12tr_TR
dc.language.isoenen_US
dc.publisherBursa Uludağ Üniversitesitr_TR
dc.relation.journalJournal of Biological and Environmental Sciencestr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectProteaseen_US
dc.subjectBacillusen_US
dc.subjectUVen_US
dc.subjectMutanten_US
dc.subjectNutritional Factorsen_US
dc.subjectOptimizationen_US
dc.titleStrain improvement by UV mutagenesis for protease overproduction from bacillus subtilis E6-5 and nutritional optimizationen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
12_35_5.pdf
Size:
473.72 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

2018 Cilt 12 Sayı 35