First determination of azole resistance in Aspergillus fumigatus strains carrying the TR34/L98H mutations in Turkey

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dc.contributor.buuauthorÖzmerdiven, Gülşah Ece
dc.contributor.buuauthorAk, Seçil
dc.contributor.buuauthorEner, Beyza
dc.contributor.buuauthorAğca, Harun
dc.contributor.buuauthorCilo, Burcu Dalyan
dc.contributor.buuauthorTunca, Berrin
dc.contributor.buuauthorAkalın, Halis
dc.contributor.departmentUludağ Üniversitesi/Tıp Fakültesi/Tıbbi Mikrobiyoloji Anabilim Dalı.tr_TR
dc.contributor.departmentUludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyoloji Anabilim Dalı.tr_TR
dc.contributor.departmentUludağ Üniversitesi/Tıp Fakültesi/Klinik Mikrobiyoloji ve Enfeksiyon Hastalıkları Anabilim Dalı.tr_TR
dc.contributor.orcid0000-0002-4803-8206tr_TR
dc.contributor.orcid000-0002-1619-6680tr_TR
dc.contributor.orcid0000-0002-2651-2034tr_TR
dc.contributor.researcheridAAH-4027-2021tr_TR
dc.contributor.researcheridAAU-8952-2020tr_TR
dc.contributor.researcheridAAG-8523-2021tr_TR
dc.contributor.researcheridABI-6078-2020tr_TR
dc.contributor.scopusid56364250700tr_TR
dc.contributor.scopusid55253485700tr_TR
dc.contributor.scopusid15053025300tr_TR
dc.contributor.scopusid15759379900tr_TR
dc.contributor.scopusid56364338600tr_TR
dc.contributor.scopusid6602965754tr_TR
dc.contributor.scopusid57207553671tr_TR
dc.date.accessioned2022-06-10T06:11:08Z
dc.date.available2022-06-10T06:11:08Z
dc.date.issued2015-08
dc.description.abstractAspergillus fumigatus is the most important etiological agent of invasive aspergillosis. Recently, an increasing number of azole-resistant A. fumigatus isolates have been described in various countries. The prevalence of azole resistance was investigated in this study using our culture collection of A. fumigatus isolates collected between 1999 and 2012 from clinical specimens. Seven hundred and forty-six A. fumigatus isolates, collected from 419 patients, were investigated. First, all isolates were screened for resistance to itraconazole by subculturing on Sabouraud dextrose agar that contained 4 mg/L itraconazole. For isolates that grew on the itraconazole containing agar, the in vitro activities of amphotericin B, itraconazole, voriconazole and posaconazole were determined using the Clinical and Laboratory Standards Institute (CLSI) M38-A reference method. After PCR amplification, the full sequence of the cyp51A gene and its promoter region was determined for all in vitro azole-resistant isolates. Itraconazole resistance was found in 10.2% of the A. fumigatus isolates. From 2000 onwards, patients were observed annually with an itraconazole-resistant isolate. According to in vitro susceptibility tests, amphotericin B exhibited good activity against all isolates whereas the azoles were resistant. Sequence analysis of the promoter region and CYP51A gene indicated the presence of TR34/L98H in 86.8% (n - 66) of isolates. This initial analysis of the resistance mechanism of A. fumigatus from Turkey revealed a common TR34/L98H mutation in the cyp51A gene.en_US
dc.identifier.citationÖzmerdiven, G. E. vd. (2015). "First determination of azole resistance in Aspergillus fumigatus strains carrying the TR34/L98H mutations in Turkey". Journal of Infection and Chemotherapy, 21(8), 581-586.en_US
dc.identifier.endpage586tr_TR
dc.identifier.issn1341-321X
dc.identifier.issue8tr_TR
dc.identifier.pubmed26048062tr_TR
dc.identifier.scopus2-s2.0-84937633798tr_TR
dc.identifier.startpage581tr_TR
dc.identifier.urihttps://doi.org/10.1016/j.jiac.2015.04.012
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S1341321X15001051
dc.identifier.urihttp://hdl.handle.net/11452/27017
dc.identifier.volume21tr_TR
dc.identifier.wos000361171400006tr_TR
dc.indexed.pubmedPubMeden_US
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.bapKUAP[T] 2013/4tr_TR
dc.relation.journalJournal of Infection and Chemotherapyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAspergillus fumigatusen_US
dc.subjectAzole resistanceen_US
dc.subjectTR34/L98H mutationen_US
dc.subjectInvasive aspergillosisen_US
dc.subjectCyp51a geneen_US
dc.subjectHematological malignanciesen_US
dc.subjectItraconazole resistanceen_US
dc.subjectTr/l98h mutationen_US
dc.subjectIn-vitroen_US
dc.subjectPosaconazoleen_US
dc.subjectPatienten_US
dc.subjectVoriconazoleen_US
dc.subjectProphylaxisen_US
dc.subjectInfectious diseasesen_US
dc.subjectPharmacology & pharmacyen_US
dc.subject.emtreeAmphotericin Ben_US
dc.subject.emtreeItraconazoleen_US
dc.subject.emtreePosaconazoleen_US
dc.subject.emtreeVoriconazoleen_US
dc.subject.emtreeAmphotericin Ben_US
dc.subject.emtreeAntifungal agenten_US
dc.subject.emtreeCytochrome P-450 CYP51A, Aspergillusen_US
dc.subject.emtreeCytochrome P450en_US
dc.subject.emtreeFungal proteinen_US
dc.subject.emtreeItraconazoleen_US
dc.subject.emtreePosaconazoleen_US
dc.subject.emtreeTriazole derivativeen_US
dc.subject.emtreeVoriconazoleen_US
dc.subject.emtreeAdulten_US
dc.subject.emtreeAgeden_US
dc.subject.emtreeAntifungal resistanceen_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeAspergillomaen_US
dc.subject.emtreeAspergillus fumigatusen_US
dc.subject.emtreeClinical articleen_US
dc.subject.emtreeCyp51A geneen_US
dc.subject.emtreeFemaleen_US
dc.subject.emtreeFungal geneen_US
dc.subject.emtreeFungal strainen_US
dc.subject.emtreeFungus isolationen_US
dc.subject.emtreeFungus mutationen_US
dc.subject.emtreeHumanen_US
dc.subject.emtreeIn vitro studyen_US
dc.subject.emtreeLung aspergillosisen_US
dc.subject.emtreeMaleen_US
dc.subject.emtreeNonhumanen_US
dc.subject.emtreePolymerase chain reactionen_US
dc.subject.emtreePromoter regionen_US
dc.subject.emtreeSequence analysisen_US
dc.subject.emtreeTR34L98H geneen_US
dc.subject.emtreeTurkey (republic)en_US
dc.subject.emtreeAspergillosisen_US
dc.subject.emtreeAspergillus fumigatusen_US
dc.subject.emtreeDna mutational analysisen_US
dc.subject.emtreeDrug effectsen_US
dc.subject.emtreeGeneticsen_US
dc.subject.emtreeMicrobial sensitivity testen_US
dc.subject.emtreeMiddle ageden_US
dc.subject.emtreeMutationen_US
dc.subject.emtreeTurkeyen_US
dc.subject.emtreeYoung adulten_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAmphotericin Ben_US
dc.subject.meshAntifungal agentsen_US
dc.subject.meshAspergillosisen_US
dc.subject.meshAspergillus fumigatusen_US
dc.subject.meshCytochrome P-450 enzyme systemen_US
dc.subject.meshDNA mutational analysisen_US
dc.subject.meshDrug resistance, fungalen_US
dc.subject.meshFemaleen_US
dc.subject.meshFungal proteinsen_US
dc.subject.meshHumansen_US
dc.subject.meshItraconazoleen_US
dc.subject.meshMaleen_US
dc.subject.meshMicrobial sensitivity testsen_US
dc.subject.meshMiddle ageden_US
dc.subject.meshMutationen_US
dc.subject.meshPromoter regions, geneticen_US
dc.subject.meshTriazolesen_US
dc.subject.meshTurkeyen_US
dc.subject.meshVoriconazoleen_US
dc.subject.meshYoung adulten_US
dc.subject.scopusIsavuconazonium; Antifungal Agents; Voriconazoleen_US
dc.subject.wosInfectious diseasesen_US
dc.subject.wosPharmacology & pharmacyen_US
dc.titleFirst determination of azole resistance in Aspergillus fumigatus strains carrying the TR34/L98H mutations in Turkeyen_US
dc.typeArticle
dc.wos.quartileQ3 (Infectious diseases)en_US
dc.wos.quartileQ4 (Pharmacology & pharmacy)en_US

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