RT-PCR assays for seven serotypes of epizootic haemorrhagic disease virus & their use to type strains from the mediterranean region and North America

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dc.contributor.authorMaan, Narender Singh
dc.contributor.authorMaan, Sushila
dc.contributor.authorNomikou, Kyriaki
dc.contributor.authorJohnson, Donna J.
dc.contributor.authorEl-Harrak, Mehdi
dc.contributor.authorMadani, Hafsa
dc.contributor.authorYadin, Hagai
dc.contributor.authorİnçoğlu, Şerife
dc.contributor.authorAllison, Andrew B.
dc.contributor.authorStallknecht, David E.
dc.contributor.authorBatten, Carrie Anne
dc.contributor.authorAnthony, Simon John
dc.contributor.authorMertens, Peter Paul Clement
dc.contributor.buuauthorYeşilbağ, Kadir
dc.contributor.departmentUludağ Üniversitesi/Veterinerlik Fakültesi/Klinik Öncesi Bilimler Bölümü.tr_TR
dc.contributor.researcheridABE-7662-2020tr_TR
dc.contributor.scopusid6602912127tr_TR
dc.date.accessioned2022-09-14T11:55:25Z
dc.date.available2022-09-14T11:55:25Z
dc.date.issued2010-09-17
dc.description.abstractEpizootic haemorrhagic disease virus (EHDV) infects wild ruminants, causing a frequently fatal haemorrhagic disease. However, it can also cause bluetongue-like disease in cattle, involving significant levels of morbidity and mortality, highlighting a need for more rapid and reliable diagnostic assays. EHDV outer-capsid protein VP2 (encoded by genomesegment 2 [Seg-2]) is highly variable and represents the primary target for neutralising antibodies generated by the mammalian host. Consequently VP2 is also the primary determinant of virus "serotype", as identified in virus neutralisation tests (VNT). Although previous reports have indicated eight to ten EHDV serotypes, recent serological comparisons and molecular analyses of Seg-2 indicate only seven EHDV "types". Oligonucleotide primers were developed targeting Seg-2, for use in conventional RT-PCR assays to detect and identify these seven types. These assays, which are more rapid and sensitive, still show complete agreement with VNT and were used to identify recent EHDV isolates from the Mediterranean region and North America.en_US
dc.description.sponsorshipUK Research & Innovation (UKRI) Biotechnology and Biological Sciences Research Council (BBSRC)en_US
dc.description.sponsorshipCGIAR European Commission (SANCO/940/2002)en_US
dc.description.sponsorshipDepartment for Environment, Food & Rural Affairs (DEFRA) (SE2617)en_US
dc.description.sponsorshipEuropean Commission European Commission Joint Research Centreen_US
dc.description.sponsorshipUK Research & Innovation (UKRI) Biotechnology and Biological Sciences Research Council (BBSRC) (BBS/E/I/00001159)en_US
dc.identifier.citationMaan, N. S. vd. (2010). "RT-PCR assays for seven serotypes of epizootic haemorrhagic disease virus & their use to type strains from the mediterranean region and North America". Plos One, 5(9), 1-13.en_US
dc.identifier.endpage13tr_TR
dc.identifier.issn1932-6203
dc.identifier.issue9tr_TR
dc.identifier.scopus2-s2.0-77958567353tr_TR
dc.identifier.startpage1tr_TR
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0012782
dc.identifier.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0012782
dc.identifier.urihttp://hdl.handle.net/11452/28721
dc.identifier.volume5tr_TR
dc.identifier.wos000281960800011tr_TR
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherPublic Library Scienceen_US
dc.relation.collaborationYurt içitr_TR
dc.relation.collaborationYurt dışıtr_TR
dc.relation.collaborationSanayitr_TR
dc.relation.journalPlos Oneen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectWhite-tailed deeren_US
dc.subjectPolymerase chain-reactionen_US
dc.subjectBluetongue-virusen_US
dc.subjectCompetitive elisaen_US
dc.subjectIbaraki-virusen_US
dc.subjectSequence-analysisen_US
dc.subjectGenomeen_US
dc.subjectDifferentiationen_US
dc.subjectAntibodiesen_US
dc.subjectProteinsen_US
dc.subjectScience & technology - other topicsen_US
dc.subjectBosen_US
dc.subjectEpizootic hemorrhagic disease virusen_US
dc.subjectMammaliaen_US
dc.subjectMiridaeen_US
dc.subject.emtreeNeutralizing antibodyen_US
dc.subject.emtreeOligonucleotideen_US
dc.subject.emtreeProtein VP2en_US
dc.subject.emtreeCapsid proteinen_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeControlled studyen_US
dc.subject.emtreeMammalen_US
dc.subject.emtreeNonhumanen_US
dc.subject.emtreeNorth Americaen_US
dc.subject.emtreeNucleotide sequenceen_US
dc.subject.emtreeOrbivirusen_US
dc.subject.emtreeProtein targetingen_US
dc.subject.emtreeReverse transcription polymerase chain reactionen_US
dc.subject.emtreeSeg 2 geneen_US
dc.subject.emtreeSerologyen_US
dc.subject.emtreeSerotypeen_US
dc.subject.emtreeSouthern Europeen_US
dc.subject.emtreeVirus geneen_US
dc.subject.emtreeVirus genomeen_US
dc.subject.emtreeVirus isolationen_US
dc.subject.emtreeVirus neutralizationen_US
dc.subject.emtreeVirus strainen_US
dc.subject.emtreeVirus typingen_US
dc.subject.emtreeAnimalsen_US
dc.subject.emtreeAnimal diseaseen_US
dc.subject.emtreeCattleen_US
dc.subject.emtreeCattle diseaseen_US
dc.subject.emtreeCell lineen_US
dc.subject.emtreeClassificationen_US
dc.subject.emtreeEvaluationen_US
dc.subject.emtreeGeneticsen_US
dc.subject.emtreeHamsteren_US
dc.subject.emtreeIsolation and purificationen_US
dc.subject.emtreeMethodologyen_US
dc.subject.emtreeMolecular geneticsen_US
dc.subject.emtreePhylogenyen_US
dc.subject.emtreeReovirus infectionen_US
dc.subject.emtreeVirologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCapsid proteinsen_US
dc.subject.meshCattleen_US
dc.subject.meshCattle diseaseen_US
dc.subject.meshCell lineen_US
dc.subject.meshCricetinaeen_US
dc.subject.meshHemorrhagic disease virus, epizooticen_US
dc.subject.meshMediterranean regionen_US
dc.subject.meshMolecular sequence dataen_US
dc.subject.meshNorth americaen_US
dc.subject.meshPhylogenyen_US
dc.subject.meshReoviridae infectionsen_US
dc.subject.meshReverse transcriptase polymerase chain reactionen_US
dc.subject.scopusSerotype; Epizootic Hemorrhagic Disease Virus; African Horse Sickness Virusen_US
dc.subject.wosMultidisciplinary sciencesen_US
dc.titleRT-PCR assays for seven serotypes of epizootic haemorrhagic disease virus & their use to type strains from the mediterranean region and North Americaen_US
dc.typeArticle
dc.wos.quartileQ1en_US

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