Characterisation of autolytic enzymes in Lactobacillus pentosus
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Dosyalar
Tarih
2004
Kurum Yazarları
Chartier, Marie Pierre Chapot
Süreli Yayın başlığı
Süreli Yayın ISSN
Cilt Başlığı
Yayınevi
Wiley
Özet
Aims: To characterize autolysis and autolytic system of the lactic acid bacterium Lactobacillus pentosus. Methods and Results: Autolysis of nine Lact. pentosus strains was evaluated in buffer solution. Their peptidoglycan hydrolase profiles were examined by renaturing SDS-PAGE and revealed two major activity bands at 58 and 112 kDa. Specificity analysis indicated the presence of at least two different types of peptidoglycan hydrolase activities in Lact. pentosus 1091. Conclusions: Autolysis of Lact. pentosus was shown to be strain dependent and involvement of at least two different autolysins was evidenced. Significance and Impact of the Study: The autolytic system of Lact. pentosus was characterized for the first time and the data obtained could be used in the selection of strains of technological interest.
Açıklama
Anahtar kelimeler
Biotechnology and applied microbiology, Microbiology, Bacteria (microorganisms), Lactobacillus, Lactobacillus pentosus, Posibacteria, Autolysis, Nonstarter lactic acid bacteria, Peptidoglycan hydrolase, Renaturing SDS-PAGE, Lactic-acid bacteria, Lactococcus-lactis, Cheese, Strains, Identification, Proteolysis, Phenotype, Prophage, Cheddar
Alıntı
Çibik, R. ve Chartier, M. P. C. (2004). “Characterisation of autolytic enzymes in Lactobacillus pentosus”. Letters in Applied Microbiology, 38(6), 459-463.