Degradation of phenol in nutrient broth by pseudomonas and bacillus species using biosimulator

Abstract

Phenol is a cognizant industrial contaminant in wastewaters from various industries, in this bioremedial study three isolated cultures IES.S, IES.Ps and IES.B separately and all in consortia (10%) from industrial wastes were grown in 1500 ml nutrient broth in sterile condition with 600 mg/l Phenol in the Biotron fermenter vessel, with specific conditions of rpm, DO. pH, temperature, COD and phenol was determined at specific interval of time. The results of this study showed that the pH of the system mostly remained acidic. The DO concentration was sustained at 5.0 mg/l. Phenol removal by IES.S was 15.50% (42 hours) and 18.83% (90 hours). Phenol removal percentage was much higher with IES.Ps as compared to that of IES.S. After 42 hours the phenol percentage removal efficiency was 32.10%. While at the end of the experimental run about 40% phenol was removed. While using IES.B Phenol removal efficiency was 32.16 (42 hours) and 39.53 % (90 hours). Phenol removal efficiency was relatively higher than IES.S and relatively lower than IES.Ps. Phenol removal was 31.57% (42 hours) and 39.38 % (90 hours) by using consortia of all three cultures. The degradation rates by using three different isolates were in descending order Consortium>IES.Ps>IES.B>IES.S.