Flow sitometri ve bitki ıslahı
Loading...
Date
2020-12-20
Authors
Demirel, Fatih
Eren, Barış
Demirel, Serap
Erol, Aybüke
Journal Title
Journal ISSN
Volume Title
Publisher
Bursa Uludağ Üniversitesi
Abstract
Bitkiler hem insanoğlunun hem de hayvanların beslenmesinde önemli bir yere sahiptir. Artan dünya popülasyonu ve küresel ısınma besin kalitesi ve kaynağı üzerinde problemler oluşturmaktadır. Bitki ıslahı besin kalitesinin ve verimin arttırılması amacıyla birçok bitkide uygulanmıştır. Flow sitometri (flow cytometry; FCM) keşfedildiğinden beri bitki ıslahında ploidi seviyelerinin belirlenmesi için uygun ve kullanışlı bir araç olmuştur. Flow sitometri ebeveyn bitkilerin karakterize edilmesi, çaprazlama sonucu oluşan yavru bitkilerin kromozom içeriğinin saptanması, poliploidizasyon çalışmaları gibi farklı amaçlar doğrultusunda kullanılmaktadır. Bu çalışma da flow sitometri ve bitki ıslahında kullanımı ele alınmıştır. Teknolojinin gelişmesi ile birlikte flow sitometrinin tarımda ve bitki ıslahında kullanımı yaygınlaşmıştır. Araştırmacıların genom modifikasyonu üzerindeki çalışmalara ilgisi arttıkça, flow sitometrinin farklı uygulamalarda kullanılacağı olası gözükmektedir.
Plants have a cruial place in the nutrition of both the humankind and the animals. Increasing world population and global warming create problems on food quality and sources. Plant breeding has been applied in many plants in order to increase nutrient quality and yield. Since flow cytometry was discovered, it has been a convenient and useful tool for determining ploidy levels in plant breeding. Flow cytometry is used for different purposes such as characterization of parent plants, determination of chromosome content of offspring plants and polyploidization studies in breeding. In this study flow cytometry and its use in plant breeding are discussed. With the development of technology and automated systems, the use of flow cytometry in agriculture and plant breeding has become widespread. As researchers have increased interest in genome modification studies, it is likely that flow cytometry will be used in different applications.
Plants have a cruial place in the nutrition of both the humankind and the animals. Increasing world population and global warming create problems on food quality and sources. Plant breeding has been applied in many plants in order to increase nutrient quality and yield. Since flow cytometry was discovered, it has been a convenient and useful tool for determining ploidy levels in plant breeding. Flow cytometry is used for different purposes such as characterization of parent plants, determination of chromosome content of offspring plants and polyploidization studies in breeding. In this study flow cytometry and its use in plant breeding are discussed. With the development of technology and automated systems, the use of flow cytometry in agriculture and plant breeding has become widespread. As researchers have increased interest in genome modification studies, it is likely that flow cytometry will be used in different applications.
Description
Keywords
Bitki, Breeding, DNA miktarı, DNA amount, Islah, FCM, Plant
Citation
Demirel, F. , vd (2020). "Flow sitometri ve bitki ıslahı". Bursa Uludağ Üniversitesi Ziraat Fakültesi Dergisi, 34(1), 213-223.