4-(N-hydroxyphenyl)retinamide can selectively induce apoptosis in human epidermoid carcinoma cells but not in normal dermal fibroblasts

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dc.contributor.authorWood, E. J.
dc.contributor.buuauthorUlukaya, Engin
dc.contributor.buuauthorKurt, Ayberk
dc.contributor.departmentUludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.tr_TR
dc.contributor.researcheridK-5792-2018tr_TR
dc.contributor.scopusid6602927353tr_TR
dc.contributor.scopusid36615287100tr_TR
dc.date.accessioned2021-10-08T06:25:20Z
dc.date.available2021-10-08T06:25:20Z
dc.date.issued2001
dc.description.abstractThe retinoid 4-(N-hydroxyphenyl)retinamide (4HPR, fenretinide) has both growth inhibitory and apoptosis-inducing effects on a number of cancer cell lines in vitro and in vivo and has been entered into a number of oncological trials. However, little is known about its mechanism(s) of action or its effects on normal cells such as fibroblasts. In this study, the effects of fenretinide on both epidermoid carcinoma cells of vulva (cell line A431) and normal human dermal fibroblasts, both as monolayers and also grown in 3D cell culture systems, have been investigated. The 3D cell culture system contained normal human fibroblasts embedded in a type I collagen gel with the carcinoma cells seeded on top of the collagen gel, which mimics the epidermoid carcinoma. Fenretinide significantly inhibited the rate of DNA synthesis of carcinoma cells, while there was little effect on fibroblasts on monolayers, at 10(-6)-10(-5) M, which are clinically attainable does. Fenretinide at 5 x 10(-6) M induced apoptosis characterised by cell shrinkage, membrane blebbing, nuclear condensation and/or fragmentation, and cell detachment in carcinoma cells, but not fibroblasts from monolayers. Fenretinide also reduced the viability of carcinoma cells in the 3D cell culture system without affecting fibroblasts. These data show that fenretinide may preferentially induce apoptosis in epidermoid carcinoma cells.en_US
dc.identifier.citationUlukaya, E. vd. (2001). "4-(N-hydroxyphenyl)retinamide can selectively induce apoptosis in human epidermoid carcinoma cells but not in normal dermal fibroblasts". Cancer Investigation, 19(2), 145-154.en_US
dc.identifier.endpage154tr_TR
dc.identifier.issn0735-7907
dc.identifier.issue2tr_TR
dc.identifier.pubmed11296619tr_TR
dc.identifier.scopus2-s2.0-0035081151tr_TR
dc.identifier.startpage145tr_TR
dc.identifier.urihttps://doi.org/10.1081/CNV-100000149
dc.identifier.urihttps://www.tandfonline.com/doi/full/10.1081/CNV-100000149
dc.identifier.urihttp://hdl.handle.net/11452/22290
dc.identifier.volume19tr_TR
dc.identifier.wos000167698300005tr_TR
dc.indexed.pubmedPubmeden_US
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherTaylor & Francisen_US
dc.relation.collaborationYurt dışıtr_TR
dc.relation.journalCancer Investigationen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectFenretinide 4-(N-hydroxyphenyl)retinamideen_US
dc.subjectEpidermoid carcinora cellsen_US
dc.subjectApoptosisen_US
dc.subjectN-(4-Hydroxyphenyl)retinamideen_US
dc.subjectTrans-retinoic aciden_US
dc.subjectGrowth-inhibitionen_US
dc.subjectRetınamideen_US
dc.subjectLinesen_US
dc.subjectChemopreventionen_US
dc.subjectFenretinideen_US
dc.subjectPathwaysen_US
dc.subject4-Hpren_US
dc.subjectHeaden_US
dc.subjectOncologyen_US
dc.subject.emtreeFibroblasten_US
dc.subject.emtreeApoptosis inducing factoren_US
dc.subject.emtreeCollagen type 1en_US
dc.subject.emtreeApoptosisen_US
dc.subject.emtreeFenretinideen_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeCancer cell cultureen_US
dc.subject.emtreeCell viabilityen_US
dc.subject.emtreeControlled studyen_US
dc.subject.emtreeDNA synthesisen_US
dc.subject.emtreeDrug mechanismen_US
dc.subject.emtreeHumanen_US
dc.subject.emtreeGrowth inhibitionen_US
dc.subject.emtreeHuman cellen_US
dc.subject.emtreeIn vivo studyen_US
dc.subject.emtreeIn vitro studyen_US
dc.subject.emtreeMonolayer cultureen_US
dc.subject.emtreePriority journalen_US
dc.subject.emtreeSquamous cell carcinomaen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntineoplastic agentsen_US
dc.subject.meshApoptosisen_US
dc.subject.meshCarcinoma, squamous cellen_US
dc.subject.meshCell culture techniquesen_US
dc.subject.meshCell survivalen_US
dc.subject.meshCollagenen_US
dc.subject.meshDNA fragmentationen_US
dc.subject.meshFenretinideen_US
dc.subject.meshFibroblastsen_US
dc.subject.meshHumansen_US
dc.subject.meshNucleosomesen_US
dc.subject.meshRatsen_US
dc.subject.meshReference valuesen_US
dc.subject.meshSkinen_US
dc.subject.meshTumor cells, cultureden_US
dc.subject.scopusFenretinide; N-(4-Methoxyphenyl)Retinamide; Neuroblastomaen_US
dc.subject.wosOncologyen_US
dc.title4-(N-hydroxyphenyl)retinamide can selectively induce apoptosis in human epidermoid carcinoma cells but not in normal dermal fibroblastsen_US
dc.typeArticle
dc.wos.quartileQ3en_US

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