Serum biochemical profile of broiler chickens fed diets containing rosemary and rosemary volatile oil

Abstract

The study was conducted to determine the effects of dietary supplementation rosemary aromatic plant, rosemary volatile oil and α-tocopherol acetate (Vitamin E) on serum variables of broilers fed on maize-soybean meal based diets. Eight hundred 1-d-old Ross-308 male chickens were weighed and randomly divided into 1 control and 7 experimental groups each with 10 replicates of 10 birds. There were 8 dietary treatments: (VitE1) control without rosemary and rosemary volatile oil only with 50 mg/kg vitamin E; (R1) 5.7 g/kg ground rosemary leaves; (R2) 8.6 g/kg ground rosemary leaves; (R3) 11.5 g/kg ground rosemary leaves; (RO1) 100 mg/kg rosemary volatile oil; (RO2) 150 mg/kg rosemary volatile oil; (RO3) 200 mg/kg rosemary volatile oil and (VitE2) 200 mg/kg vitamin E. Broilers consumed the diets and water ad libitum. After 42 days, 80 animals were randomly selected for serum biochemical profile analysis involving ceruloplasmin, superoxide dismutase activity (SOD), transferring, albumin globulins ratio (A/G), total cholesterol, creatin, urea, alanine aminotransferase (ALT) and aspartate amino transferase (AST). While serum transferrin, urea level and ALT-AST activity were not statistically different among groups serum ceruloplasmin (p< 0.000), SOD activity (p<0.05), albumin/globulin ratio (p< 0.000), total cholesterol (p<0.001), creatinin (p<0.05) and AST (p< 0.000) level were found to be significantly different. In conclusion, the Rosmarinus officinalis plant and its volatile oil have increasing effect on serum SOD activity and effect positively oxidation mechanism. On the other hand, it can be assumed that rosemary plant created hypocholesterolemic effect in this study.