Crystallization studies of a low molecular weight xylanase from scytalidium thermophilum

dc.contributor.authorKocabaş, Didem Sutay
dc.contributor.authorYurtdaş, Eren
dc.date.accessioned2021-05-20T08:59:06Z
dc.date.available2021-05-20T08:59:06Z
dc.date.issued2017-12-12
dc.description.abstractXylanases are hydrolytic enzymes which lately attracted increasing attention due to their broad use in different industrial processes. Scytalidium thermophilum xylanase was previously shown by our research group to be a potential candidate for the industry. Therefore, the aim of this study was to optimize the crystal growth conditions of xylanase for further structural studies. Initial crystallization conditions were screened by using commercial kits with sitting drop vapor diffusion method. Consequently, optimization studies were carried out by changing the xylanase concentration, pH, reservoir solution to xylanase ratio in the drop and precipitant concentration. As a result of the study, the best xylanase crystal was obtained from the solution containing 1.8 M ammonium citrate dibasic, 0.1 M sodium acetate trihydrate at pH 4.6 and 2 mg/ml enzyme at 18°C with streak seeding. The largest and well shaped single xylanase crystal at a size of 320 µm with a light-brown color, which has the potential as a starting point of further X-ray diffraction analysis and structural studies, was obtained.
dc.identifier.citationKocabaş, D. S. ve Yurtdaş, E. (2017). "Crystallization studies of a low molecular weight xylanase from scytalidium thermophilum". Journal of Biological and Environmental Sciences, 11(33), 129-136.
dc.identifier.endpage136
dc.identifier.issn1307-9530
dc.identifier.issn1308-2019
dc.identifier.issue33
dc.identifier.startpage129
dc.identifier.urihttp://jbes.uludag.edu.tr/PDFDOSYALAR/33/mak03.pdf
dc.identifier.urihttp://hdl.handle.net/11452/20096
dc.identifier.volume11
dc.language.isoen
dc.publisherUludağ Üniversitesi
dc.relation.journalJournal of Biological and Environmental Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.relation.tubitak110M615
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectScytalidium thermophilum
dc.subjectProtein crystallization
dc.subjectXylanase
dc.titleCrystallization studies of a low molecular weight xylanase from scytalidium thermophilum
dc.typeArticle

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