Heterologous expression and purification of nisa, the precursor peptide of lantibiotic nisin from lactococcus lactis

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dc.contributor.authorNarbad, Arjan
dc.contributor.buuauthorKarakaş, Asuman Şen
dc.contributor.departmentUludağ Üniversitesi/Yenişehir Meslek Yüksekokulu/Gıda Teknolojisi Bölümü.tr_TR
dc.contributor.researcheridAAI-4483-2021tr_TR
dc.contributor.scopusid6506180901tr_TR
dc.date.accessioned2022-05-13T13:05:37Z
dc.date.available2022-05-13T13:05:37Z
dc.date.issued2012-06
dc.description.abstractThe lantibiotic nisin is a ribosomally synthesised and post-translationally modified antimicrobial peptide produced by strains of Lactococcus lactis, and used as safe and natural preservative in food industry. The nisA structural gene encodes ribosomally synthesised and biologically inactive a 57 amino acid precursor peptide (NisA) which undergoes several post-translational modifications. In this study, we report the expression of precursor nisin as a His6-tagged peptide in Escherichia coli and its purification using a nickel affinity column. The technique of spliced-overlap extension PCR was used to amplify the nisA gene and the T7 promoter region of pET-15b vector. This approach was used to introduce six histidine residues at the C-terminus of prenisin. The identity of the expressed peptide was confirmed by N-terminal sequencing. The expressed His-tagged prenisin was purified under denaturing conditions, and named as prenisin-His6. The purified prenisin-His6 was analyzed by SDS-PAGE, Western blotting and mass spectroscopy. These results showed that the nisin precursor peptide can be successfully produced using an E. coli expression system.en_US
dc.description.sponsorshipUK Research & Innovation (UKRI)en_US
dc.description.sponsorshipBiotechnology and Biological Sciences Research Council (BBSRC) - BBS/E/F/00044453en_US
dc.identifier.citationKarakaş Ş. A. ve Narbad, A. (2012). "Heterologous expression and purification of nisa, the precursor peptide of lantibiotic nisin from lactococcus lactis". Acta Biologica Hungarica, 63(2), 301-310.en_US
dc.identifier.endpage310tr_TR
dc.identifier.issn0236-5383
dc.identifier.issue2tr_TR
dc.identifier.pubmed22695527tr_TR
dc.identifier.scopus2-s2.0-84867658895tr_TR
dc.identifier.startpage301tr_TR
dc.identifier.urihttps://doi.org/10.1556/ABiol.63.2012.2.11
dc.identifier.urihttps://akjournals.com/view/journals/018/63/2/article-p301.xml
dc.identifier.urihttp://hdl.handle.net/11452/26462
dc.identifier.volume63tr_TR
dc.identifier.wos000306504800011tr_TR
dc.indexed.pubmedPubMeden_US
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherAkademiai Kiado Zrthu
dc.relation.collaborationSanayitr_TR
dc.relation.journalActa Biologica Hungaricaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLife sciences & biomedicine - other topicsen_US
dc.subjectNisinen_US
dc.subjectPrenisinen_US
dc.subjectHis-tagen_US
dc.subjectPurificationen_US
dc.subjectSpliced overlap extensionen_US
dc.subjectPosttranslational modificationen_US
dc.subjectEscherichia-colien_US
dc.subjectBiosynthesisen_US
dc.subjectGeneen_US
dc.subjectDehydrationen_US
dc.subjectProteinsen_US
dc.subjectCloningen_US
dc.subject.emtreeBacterial proteinen_US
dc.subject.emtreeHistidineen_US
dc.subject.emtreeNis a proteinen_US
dc.subject.emtreeNisinen_US
dc.subject.emtreePolyhistidine tagen_US
dc.subject.emtreeProtein precursoren_US
dc.subject.emtreeUnclassified drugen_US
dc.subject.emtreeBacteriocinen_US
dc.subject.emtreeHybrid proteinen_US
dc.subject.emtreeNisin aen_US
dc.subject.emtreeAmino acid sequenceen_US
dc.subject.emtreeAmino terminal sequenceen_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeBacterial geneen_US
dc.subject.emtreeCarboxy terminal sequenceen_US
dc.subject.emtreeEscherichia colien_US
dc.subject.emtreeExpression vectoren_US
dc.subject.emtreeGene amplificationen_US
dc.subject.emtreeHeterologous expressionen_US
dc.subject.emtreeLactococcus lactisen_US
dc.subject.emtreeMass spectrometryen_US
dc.subject.emtreeNonhumanen_US
dc.subject.emtreePolyacrylamide gel electrophoresisen_US
dc.subject.emtreePolymerase chain reactionen_US
dc.subject.emtreePromoter regionen_US
dc.subject.emtreeProtein denaturationen_US
dc.subject.emtreeProtein expressionen_US
dc.subject.emtreeProtein purificationen_US
dc.subject.emtreeWestern blottingen_US
dc.subject.emtreeAffinity chromatographyen_US
dc.subject.emtreeBiosynthesisen_US
dc.subject.emtreeGene vectoren_US
dc.subject.emtreeGeneticsen_US
dc.subject.emtreeIsolation and purificationen_US
dc.subject.emtreeMetabolismen_US
dc.subject.emtreePlasmiden_US
dc.subject.meshBacteriocinsen_US
dc.subject.meshChromatography, affinityen_US
dc.subject.meshEscherichia colien_US
dc.subject.meshGenetic vectorsen_US
dc.subject.meshLactococcus lactisen_US
dc.subject.meshNisinen_US
dc.subject.meshPlasmidsen_US
dc.subject.meshRecombinant fusion proteinsen_US
dc.subject.scopusBacteriocins; Nisin; Mutacin 1140en_US
dc.subject.wosBiologyen_US
dc.titleHeterologous expression and purification of nisa, the precursor peptide of lantibiotic nisin from lactococcus lactisen_US
dc.typeArticle
dc.wos.quartileQ4en_US

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