Immunocharacterization of matrix metalloproteinase-2 and matrix metalloproteinase-9 in canine transmissible venereal tumors

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dc.contributor.buuauthorAkkoç, Ahmet
dc.contributor.buuauthorNak, Deniz
dc.contributor.buuauthorDemirer, Aylin
dc.contributor.buuauthorŞimşek, Gözde
dc.contributor.departmentUludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı.tr_TR
dc.contributor.departmentUludağ Üniversitesi/Veteriner Fakültesi.tr_TR
dc.contributor.orcid0000-0002-5090-7917tr_TR
dc.contributor.scopusid55584229300tr_TR
dc.contributor.scopusid9280090000tr_TR
dc.contributor.scopusid54402527300tr_TR
dc.contributor.scopusid26435497200tr_TR
dc.date.accessioned2023-01-05T09:05:43Z
dc.date.available2023-01-05T09:05:43Z
dc.date.issued2016-11-07
dc.description.abstractMatrix metalloproteases (MMPs) are endogenous proteases that are responsible for degradation of extracellular matrix (ECM) proteins and cell surface antigens. The breakdown of ECM participates in the local invasion and distant metastases of malignant tumors. Canine transmissible venereal tumor (CTVT) is a naturally occurring contagious round cell neoplasm of dogs that affects mainly the external genitalia of both sexes. CTVT generally is a locally invasive tumor, but distant metastases also are common in puppies and immunocompromised dogs. We investigated the immune expressions and activities of MMP-2 and MMP-9 in CTVT. The presence of these enzymes in tumor cells and tissue homogenates was demonstrated by immunohistochemistry and western blotting. We used gelatin substrate zymography to evaluate the activities of MMP-2 and MMP-9 enzymes in tumor homogenates. We found that tumor cells expressed both MMP-2 and MMP-9. Electrophoretic bands corresponding to MMP-9 and MMP-2 were identified in immunoblots and clear bands that corresponded to the active forms of MMP-2 and MMP-9 also were detected in gelatin zymograms. Our study is the first detailed documentation of MMPs in CTVT.en_US
dc.identifier.citationAkkoç, A. vd. (2017). ''Immunocharacterization of matrix metalloproteinase-2 and matrix metalloproteinase-9 in canine transmissible venereal tumors''. Biotechnic and Histochemistry, 92(2), 100-106.en_US
dc.identifier.endpage106tr_TR
dc.identifier.issn1052-0295
dc.identifier.issue2tr_TR
dc.identifier.pubmed28296543tr_TR
dc.identifier.scopus2-s2.0-85013948725tr_TR
dc.identifier.startpage100tr_TR
dc.identifier.urihttps://doi.org/10.1080/10520295.2016.1259500
dc.identifier.urihttps://www.tandfonline.com/doi/full/10.1080/10520295.2016.1259500
dc.identifier.uri1473-7760
dc.identifier.urihttp://hdl.handle.net/11452/30275
dc.identifier.volume92tr_TR
dc.identifier.wos000398130800004tr_TR
dc.indexed.pubmedPubMeden_US
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherTaylor & Francisen_US
dc.relation.bap2009-22tr_TR
dc.relation.journalBiotechnic and Histochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.relation.tubitak109O305tr_TR
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiotechnology & applied microbiologyen_US
dc.subjectCell biologyen_US
dc.subjectCanineen_US
dc.subjectDogen_US
dc.subjectImmunohistochemistryen_US
dc.subjectMatrix metalloproteinaseen_US
dc.subjectVenereal tumoren_US
dc.subjectExpressionen_US
dc.subjectDogsen_US
dc.subjectMetastasisen_US
dc.subjectSarcomaen_US
dc.subjectCanceren_US
dc.subjectBrainen_US
dc.subjectMmp-2en_US
dc.subject.emtreeGelatinase Aen_US
dc.subject.emtreeGelatinase Ben_US
dc.subject.emtreeTissue inhibitor of metalloproteinase 2en_US
dc.subject.emtreeAnimalen_US
dc.subject.emtreeAnimal venereal tumoren_US
dc.subject.emtreeDogen_US
dc.subject.emtreeDog diseaseen_US
dc.subject.emtreeFemaleen_US
dc.subject.emtreeImmunohistochemistryen_US
dc.subject.emtreeImmunologyen_US
dc.subject.emtreeMaleen_US
dc.subject.emtreeMetabolismen_US
dc.subject.emtreePathologyen_US
dc.subject.emtreeProceduresen_US
dc.subject.meshAnimalsen_US
dc.subject.meshDog diseasesen_US
dc.subject.meshDogsen_US
dc.subject.meshFemaleen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshMaleen_US
dc.subject.meshMatrix metalloproteinase 2en_US
dc.subject.meshMatrix metalloproteinase 9en_US
dc.subject.meshTissue inhibitor of metalloproteinase-2en_US
dc.subject.meshVenereal tumors, veterinaryen_US
dc.subject.scopusDog; Sarcophilus Harrisii; Devilen_US
dc.subject.wosBiotechnology & applied microbiologyen_US
dc.subject.wosCell biologyen_US
dc.titleImmunocharacterization of matrix metalloproteinase-2 and matrix metalloproteinase-9 in canine transmissible venereal tumorsen_US
dc.typeArticle
dc.wos.quartileQ4en_US

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