Real-time polymerase chain reaction for detection of mycoplasma gallisepticum in chicken trachea

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dc.contributor.buuauthorÇarlı, Kamil Tayfun
dc.contributor.buuauthorEyigör, Ayşegül
dc.contributor.departmentUludağ Üniversitesi/Veteriner Fakültesi/Besin Hijyeni ve Teknolojisi Anabilim Dalı.tr_TR
dc.contributor.departmentUludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.tr_TR
dc.contributor.researcheridAAI-1101-2021tr_TR
dc.contributor.scopusid6601971539tr_TR
dc.contributor.scopusid6602558950tr_TR
dc.date.accessioned2021-10-07T12:29:55Z
dc.date.available2021-10-07T12:29:55Z
dc.date.issued2003-07
dc.description.abstractIn this work, we describe a rapid detection procedure for Mycoplasma gallisepticum from chicken tracheal swabs by real-time polymerase chain reaction (PCR) by LightCycler system, where we were able to monitor the amplification of the newly synthesized M gallisepticum-specific PCR product as a proportionally increasing fluorescent signal by using the double-stranded DNA binding dye SYBR Green I and have identified M. gallisepticum-specific PCR products by DNA melting curve analysis by plotting the first negative derivative (-d[F1]/dT) of fluorescence over temperature. Detection limits of the PCR were found to be 3 and 3000 colony-forming units ml(-1) with pure culture of M. gallisepticum and artificially spiked samples, respectively. Out of 96 tracheal swabs, 68 were taken from live chickens and 28 were taken by scraping the mucosal surface of the trachea (SMST) of necropsied chickens. All of the 18 PCR-positive results were from the swabs taken by the SMST method, whereas all of the samples taken from live chickens were negative. Thus, the PCR with the SMST method had a sensitivity and a specificity of 64.2% (18 of 28 chickens) and 100%, respectively. The total time required for template preparation from tracheal swab samples and real-time PCR was approximately 65 min. These results indicate that real-time PCR with the LightCycler technology is a rapid and sensitive test to identify M. gallisepticum-infected flocks if a proper sampling is applied.en_US
dc.identifier.citationÇarlı, K.T. ve Eyigör, A. (2003). “Real-time polymerase chain reaction for detection of mycoplasma gallisepticum in chicken trachea”. Avian Diseases, 47(3), 712-717.en_US
dc.identifier.endpage717tr_TR
dc.identifier.issn0005-2086
dc.identifier.issue3tr_TR
dc.identifier.pubmed14562901tr_TR
dc.identifier.scopus2-s2.0-0141682982tr_TR
dc.identifier.startpage712tr_TR
dc.identifier.urihttps://doi.org/10.1637/6041
dc.identifier.urihttp://hdl.handle.net/11452/22285
dc.identifier.volume47tr_TR
dc.identifier.wos000185415100025tr_TR
dc.indexed.pubmedPubmeden_US
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherAmer Assoc Avian Pathologistsen_US
dc.relation.journalAvian Diseasesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectVeterinary sciencesen_US
dc.subjectMycoplasma gallisepticumen_US
dc.subjectPoultryen_US
dc.subjectReal-time PCRen_US
dc.subjectAvesen_US
dc.subjectBacteria (microorganisms)en_US
dc.subjectGallus gallusen_US
dc.subjectMycoplasmaen_US
dc.subjectTetrathionate broth enrichmenten_US
dc.subjectCapillary PCRen_US
dc.subjectDiagnosisen_US
dc.subjectSynoviaeen_US
dc.subjectDNAen_US
dc.subject.scopusMycoplasma Synoviae; Carpodacus Mexicanus; Poultry Industryen_US
dc.subject.wosVeterinary sciencesen_US
dc.titleReal-time polymerase chain reaction for detection of mycoplasma gallisepticum in chicken tracheaen_US
dc.typeArticle
dc.wos.quartileQ2en_US

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