P-glycoprotein expression by technetium-99m-MIBI scintigraphy in hematologic malignancy

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dc.contributor.authorKostakoğlu, Lale
dc.contributor.authorDicle, Güç
dc.contributor.authorCanpınar, Hande
dc.contributor.authorKars, Ayşe
dc.contributor.authorKıratlı, Pınar
dc.contributor.authorHayran, Mutlu
dc.contributor.authorGündüz, Ufuk
dc.contributor.authorKansu, Emin
dc.contributor.buuauthorAlper, Eray
dc.contributor.departmentUludağ Üniversitesi/Tıp Fakültesi/Nükleer Tıp Anabilim Dalı.tr_TR
dc.date.accessioned2021-06-30T11:29:42Z
dc.date.available2021-06-30T11:29:42Z
dc.date.issued1998
dc.description.abstractOur aim was to ascertain the relationship between the degree of Tc-99m-MIBI uptake and the level of p-glycoprotein (Pgp) expression determined by flow cytometry and reverse transcription-polymerase chain reaction (RT-PCR) techniques in patients with hematologic malignancy. Methods: A total of 21 samples (19 patients) were evaluated. Two patients had repeat studies after therapy. Thirteen samples were studied at the time of initial diagnosis and 8 during relapse after therapy. After MIBI imaging, either bone marrow aspiration or peripheral blood was obtained for flow cytometric and RT-PCR analyses, Flow cytometry was performed using two different antibodies. After the injection of 555 MBq MIBI, whole-body and pelvic spot images were acquired using a dual-head gamma camera, The uptake in the bone marrow was evaluated against the background (adjacent soft tissue) by both qualitative (scoring system) and quantitative (tm/bkg ratios) analyses, Results: For flow cytometry, the limit for Pgp overexpression was set at >15% Pgp-positive mononuclear bone marrow or peripheral blood cells. There was an inverse correlation between the levels of Pgp and MIBI imaging using both the qualitative (scoring system) and quantitative (tm/bkg ratios) analyses (p = 0.022), Mean values were statistically different between Pgp + and Pgp - groups for both qualitative and quantitative analyses (p = 0.009 and 0.024, respectively). For RT-PCR, there was statistical support toward a difference in the mean values between Pgp + and Pgp - groups by qualitative analysis (p = 0.061); however, no statistical difference was found between these two groups by quantitative analysis (p = 0.179), Conclusion: Based on the strong correlation between the imaging and flow cytometry and a statistical support toward the correlation between the imaging and RT-PCR, MIBI imaging may be used for the in vivo detection of Pgp in patients with hematologic malignancy.en_US
dc.identifier.citationKostakoğlu, L. vd. (1998). "P-glycoprotein expression by technetium-99m-MIBI scintigraphy in hematologic malignancy". Journal of Nuclear Medicine, 39(7), 1191-1197.tr_TR
dc.identifier.endpage1197tr_TR
dc.identifier.issn0161-5505
dc.identifier.issue7tr_TR
dc.identifier.pubmed9669392tr_TR
dc.identifier.scopus2-s2.0-0031814269tr_TR
dc.identifier.startpage1191tr_TR
dc.identifier.urihttps://www.proquest.com/docview/219225468
dc.identifier.urihttps://jnm.snmjournals.org/content/39/7/1191
dc.identifier.urihttp://hdl.handle.net/11452/20939
dc.identifier.volume39tr_TR
dc.identifier.wos000074691600019tr_TR
dc.indexed.pubmedPubmeden_US
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherSoc Nuclear Medicine Incen_US
dc.relation.collaborationYurt içitr_TR
dc.relation.collaborationYurt dışıtr_TR
dc.relation.journalJournal of Nuclear Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMultidrug resistanceen_US
dc.subjectP-glycoproteinen_US
dc.subjectTechnetium-99m-MIBIen_US
dc.subjectReverse transcription-polymerase chain reaction techniquesen_US
dc.subjectFlow cytometryen_US
dc.subjectMultidrug-resistance geneen_US
dc.subjectDrug-resistanceen_US
dc.subjectHuman-tumorsen_US
dc.subjectLeukemiaen_US
dc.subjectCellsen_US
dc.subjectFlowen_US
dc.subjectMitochondrialen_US
dc.subjectRhodamine-123en_US
dc.subjectAccumulationen_US
dc.subjectTransporteren_US
dc.subjectRadiology, nuclear medicine & medical imagingen_US
dc.subject.wosRadiology, nuclear medicine & medical imagingen_US
dc.titleP-glycoprotein expression by technetium-99m-MIBI scintigraphy in hematologic malignancyen_US
dc.typeArticle

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