Progress in recombinant polymerase nucleic acid amplification technology

Abstract

In vitro nucleic acid rapid technology is a technology that enables rapid amplification of trace nucleic acids in vitro. Since its inception, it has been widely used in molecular biology, medicine and legal identification, and it has been continuously improved to its function and adaptability. In vitro rapid amplification nucleic acid technology developed on the basis of the existing in vitro nucleic acid amplification principle. Polymerase spiral amplification is a new visual in vitro amplification technology that is continuously upgraded from existing LAMP technology. This article mainly introduces the recombinase polymerase amplification (RPA) technology, as well as recombinase-aid amplification (RAA) technology, the polymerase spiral reaction (PSR), and application prospects.