Positive effect of BSA supplemented soybean lecithin based extender on liquid storage of ram semen at 5 degrees C

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dc.contributor.buuauthorGökçe, Elif
dc.contributor.buuauthorAlçay, Selim
dc.contributor.buuauthorGül, Zülfiye
dc.contributor.departmentUludağ Üniversitesi/Veteriner Fakültesi/Döllenme ve Suni Tohumlama Anabilim Dalı.tr_TR
dc.contributor.departmentUludağ Üniversitesi/Veteriner Fakültesi/Eczacılık Anabilim Dalı.tr_TR
dc.contributor.orcid0000-0002-7678-3289tr_TR
dc.contributor.orcid0000-0002-8872-0074tr_TR
dc.contributor.researcheridAAF-9939-2020tr_TR
dc.contributor.researcheridABA-6294-2020tr_TR
dc.contributor.scopusid56779799700tr_TR
dc.contributor.scopusid56099810300tr_TR
dc.contributor.scopusid56086542900tr_TR
dc.date.accessioned2023-01-16T10:22:25Z
dc.date.available2023-01-16T10:22:25Z
dc.date.issued2017
dc.description.abstractThe aim of the study was to evaluate different BSA doses in soybean-lecithin based extenders for liquid storage of ram semen. Pooled semen was divided into four equal volumes and diluted in a single-step dilution method with BSA supplemented (BSA5, BSA7.5, BSA10 groups supplemented 5mg/mL, 7.5mg/mL, 10mg/mL BSA respectively) and BSA-free (control) extenders. Semen samples were assessed for sperm motility, plasma membrane functional integrity using hypo-osmotic swelling test (HOST), damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) at 0, 24, 48 and 72h of storage at 5 degrees C. Antioxidant activities of groups at 0 and 72h were measured with glutathionine (GSH) concentration, membrane lipid peroxidation status was analyzed using the malondialdehyde (MDA) concentration. Antioxidant capacity of extender also was measured with 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and Dichloro-dihydro-fluorescein diacetate (DCFH-DA) assays. In the study, BSA (5mg/mL) supplementation has beneficial effect on ram sperm parameters at all evaluation times. In addition, the increasing doses of BSA supplementation has a negative effect on sperm parameters especially 48 and 72h of storage.en_US
dc.description.abstract: Bu çalışmada, farklı dozlarda BSA ilave edilmiş lesitin bazlı sulandırıcının koç spermasının 5°C’ta kısa sureli saklanması üzerindeki etkisinin değerlendirilmesi amaçlandı. Çalışma amacıyla alınan spermalar pooling yapılarak dört eşit hacme bölündü ve 5mg/mL, 7.5mg/mL, 10mg/mL BSA ilave edilmiş (BSA5, BSA7.5, BSA10 sırasıyla) ve edilmemiş (kontrol) sulandırıcılarla sulandırıldı. Sulandırılmış spermanın sıcaklığı 5°C’a düştükten sonra 0, 24, 48 ve 72. saatlerde; motilite, plazma membran fonksiyonel bütünük [hipoozmotik şişme testi (HOST)] ve akrozomal hasar [FITC-Pisum sativum agglutinin (PSA-FITC)] muayeneleri yapıldı. Çalışmanın başlangıcı ve sonunda antioksidan aktivitenin ve lipid peroksidasyonun değerlendirilmesi için malondialdehyde (MDA) ve glutathionine (GSH) konsantrasyonları ölçüldü. Ayrıca 2,2-Diphenyl-1-picrylhydrazyl (DPPH) ve Dichloro-dihydro-fluorescein diacetate (DCFH-DA) yöntemleri ile her bir sulandırıcının antioksidan kapasiteleri değerlendirildi. Yapılan muayenelerin sonucunda; 5mg/mL BSA ilavesinin koç spermasının kısa süreli muhafazasında etkili olduğu gözlemlendi. Ayrıca artan BSA konsantrasyonunun spermanın soğutularak saklanmasında olumsuz etki oluşturduğu belirlendi.tr_TR
dc.identifier.citationGökçe, E. vd. (2017). ''Positive effect of BSA supplemented soybean lecithin based extender on liquid storage of ram semen at 5 degrees C''. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 64(4), 313-320.tr_TR
dc.identifier.endpage320tr_TR
dc.identifier.issn1300-0861
dc.identifier.issue4tr_TR
dc.identifier.scopus2-s2.0-85029687078tr_TR
dc.identifier.startpage313tr_TR
dc.identifier.urihttps://doi.org/10.1501/Vetfak_0000002815
dc.identifier.urihttp://vetjournal.ankara.edu.tr/tr/pub/issue/43284/525955
dc.identifier.uri1308-2817
dc.identifier.urihttp://hdl.handle.net/11452/30471
dc.identifier.volume64tr_TR
dc.identifier.wos000412202400011tr_TR
dc.indexed.scopusScopusen_US
dc.indexed.trdizinTrDizintr_TR
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherAnkara Üniversitesitr_TR
dc.relation.bapKUAP (V)-2015/55en_US
dc.relation.journalAnkara Üniversitesi Veteriner Fakültesi Dergisitr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectVeterinary sciencesen_US
dc.subjectBSAen_US
dc.subjectLecithinen_US
dc.subjectRam semenen_US
dc.subjectShort-term storageen_US
dc.subjectBovine serum-albuminen_US
dc.subjectThawed bull semenen_US
dc.subjectLipid-peren_US
dc.subjectOxidationoxidative stressen_US
dc.subjectEgg-yolken_US
dc.subjectAntioxidantsen_US
dc.subjectSpermatozoaen_US
dc.subjectIncubation resilienceen_US
dc.subjectSuperoxide-dismutaseen_US
dc.subjectDna integrityen_US
dc.subjectKısa süreli muhafazatr_TR
dc.subjectKoç spermasıtr_TR
dc.subjectLesitintr_TR
dc.subject.emtreeAgglutininen_US
dc.subject.emtreeBiological markeren_US
dc.subject.emtreeFluorescein diacetateen_US
dc.subject.emtreeFluorescein isothiocyanateen_US
dc.subject.emtreeGlutathioneen_US
dc.subject.emtreeMalonaldehydeen_US
dc.subject.emtreePhosphatidylcholineen_US
dc.subject.emtreeAcrosomeen_US
dc.subject.emtreeAgglutination testen_US
dc.subject.emtreeAntioxidant activityen_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeCell membraneen_US
dc.subject.emtreeControlled studyen_US
dc.subject.emtreeCryopreservationen_US
dc.subject.emtreeDilutionen_US
dc.subject.emtreeDPPH radical scavenging assayen_US
dc.subject.emtreeLipid peroxidation assayen_US
dc.subject.emtreeMicroscopyen_US
dc.subject.emtreeNonhumanen_US
dc.subject.emtreeOsmotic potentialen_US
dc.subject.emtreeOxidative stressen_US
dc.subject.emtreePeaen_US
dc.subject.emtreeRam (sheep)en_US
dc.subject.emtreeSperm preservationen_US
dc.subject.emtreeSpermatozoon motilityen_US
dc.subject.scopusExtender; Semen; Semen Extendersen_US
dc.subject.wosVeterinary sciencesen_US
dc.titlePositive effect of BSA supplemented soybean lecithin based extender on liquid storage of ram semen at 5 degrees Cen_US
dc.title.alternativeBSA ilave edilmiş lesitin bazlı sulandırıcının koç spermasının 5°C sıcaklıkta muhafaza edilmesine olumlu etkisitr_TR
dc.typeArticle
dc.wos.quartileQ4en_US

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