An in vitro model for the development of acquired tamoxifen resistance

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dc.contributor.buuauthorEskiler, Gamze Güney
dc.contributor.buuauthorÇeçener, Gülşah
dc.contributor.buuauthorTunca, Berrin
dc.contributor.buuauthorEgeli, Ünal
dc.contributor.departmentUludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyoloji Anabilim Dalı.tr_TR
dc.contributor.orcid0000-0002-3820-424Xtr_TR
dc.contributor.orcid0000-0001-7904-883Xtr_TR
dc.contributor.orcid0000-0002-1619-6680tr_TR
dc.contributor.orcid0000-0002-2088-9914tr_TR
dc.contributor.researcheridAAP-9988-2020tr_TR
dc.contributor.researcheridAAH-1420-2021tr_TR
dc.contributor.researcheridAAB-6011-2022tr_TR
dc.contributor.researcheridABI-6078-2020tr_TR
dc.contributor.scopusid6508156530tr_TR
dc.contributor.scopusid6602965754tr_TR
dc.contributor.scopusid57190947987tr_TR
dc.contributor.scopusid55665145000tr_TR
dc.date.accessioned2022-12-12T12:44:39Z
dc.date.available2022-12-12T12:44:39Z
dc.date.issued2016-07-15
dc.description.abstractThe development of resistance to tamoxifen (Tam) remains a challenging clinical problem for ER+ breast cancer patients. To understand the mechanisms underlying of resistance, previous studies have driven the acquisition of Tam resistance by exposing cells to varying concentration of drug for varying lengths of time. However, a detailed protocol for the establishment of Tam-resistant cells remains to be clarified. In the present study, we aimed to determine and compare the effect of different in vitro protocols on the degree of resistance to 4-hydroxytamoxifen (4-OH Tam) for MCF7 cells. For this purpose, MCF7-Tam resistance (MCF7-TamR) cells were developed by treated with different concentrations (100, 200, 400, 600, 800 and 1000 nM) of 4-OH Tam over 3 months. The relative resistance was measured by WST-1 analysis. Studies characterizing of the 4-OH Tam resistance of MCF7-TamR cells were performed by 17 beta-oestradiol (E2) and Annexin V/PI analysis. In addition, the expression levels of ABCC1, ABCG2 and ABCG1 were detected by RT-PCR, any changes in morphological of each resistance group were observed at the end of each month and compared with parental MCF7 cells. Consequently, exposure time and concentration can affect the degree of resistance to 4-OH Tam; thus, dose and treatment duration should be chosen according to the desired degree of resistance. This work presents a novel procedure for the generation of MCF7-TamR cells, thus enabling the identification and characterization of MCF7-TamR cells.en_US
dc.identifier.citationEskiler, G. G. vd. (2016). "An in vitro model for the development of acquired tamoxifen resistance". Cell Biology and Toxicology, 32(6), 563-581.en_US
dc.identifier.endpage581tr_TR
dc.identifier.issn0742-2091
dc.identifier.issn1573-6822
dc.identifier.issue6tr_TR
dc.identifier.pubmed27585693tr_TR
dc.identifier.scopus2-s2.0-84984850991tr_TR
dc.identifier.startpage563tr_TR
dc.identifier.urihttps://doi.org/10.1007/s10565-016-9355-8
dc.identifier.urihttps://link.springer.com/article/10.1007/s10565-016-9355-8
dc.identifier.urihttp://hdl.handle.net/11452/29828
dc.identifier.volume32tr_TR
dc.identifier.wos000387595200008tr_TR
dc.indexed.pubmedPubMeden_US
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.bapOUAP(T)-2014/30tr_TR
dc.relation.journalCell Biology and Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCell biologyen_US
dc.subjectToxicologyen_US
dc.subjectBreast canceren_US
dc.subjectTamoxifenen_US
dc.subjectDrug resistanceen_US
dc.subjectMCF7 cellsen_US
dc.subjectBreast-cancer cellsen_US
dc.subjectDrug-resistanceen_US
dc.subjectReceptoren_US
dc.subjectMechanismsen_US
dc.subjectApoptosisen_US
dc.subjectInductionen_US
dc.subjectExpressionen_US
dc.subjectLineen_US
dc.subjectE2f1en_US
dc.subject.emtreeABC transporter G1en_US
dc.subject.emtreeAfimoxifeneen_US
dc.subject.emtreeBreast cancer resistance proteinen_US
dc.subject.emtreeEstradiolen_US
dc.subject.emtreeEstrogenen_US
dc.subject.emtreeLipocortin 5en_US
dc.subject.emtreeMultidrug resistance associated protein 1en_US
dc.subject.emtreeTamoxifenen_US
dc.subject.emtreeABCB1 protein, humanen_US
dc.subject.emtreeEstradiolen_US
dc.subject.emtreeMessenger RNAen_US
dc.subject.emtreeMultidrug resistance proteinen_US
dc.subject.emtreeMultidrug resistance-associated protein 1en_US
dc.subject.emtreeTamoxifenen_US
dc.subject.emtreeTAP1 protein, humanen_US
dc.subject.emtreeTransporter associated with antigen processing 1en_US
dc.subject.emtreeApoptosisen_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeCancer modelen_US
dc.subject.emtreeCancer resistanceen_US
dc.subject.emtreeCell structureen_US
dc.subject.emtreeConcentration responseen_US
dc.subject.emtreeControlled studyen_US
dc.subject.emtreeGene expression profilingen_US
dc.subject.emtreeHumanen_US
dc.subject.emtreeHuman cellen_US
dc.subject.emtreeIn vitro studyen_US
dc.subject.emtreeMCF-7 cell lineen_US
dc.subject.emtreePriority jourbiological modelnalen_US
dc.subject.emtreeProtein expressionen_US
dc.subject.emtreeReverse transcription polymerase chain reactionen_US
dc.subject.emtreeTreatment durationen_US
dc.subject.emtreeAnalysis of varianceen_US
dc.subject.emtreeBiological modelen_US
dc.subject.emtreeCell shapeen_US
dc.subject.emtreeCell survivalen_US
dc.subject.emtreeDrug resistanceen_US
dc.subject.emtreeGene expression regulationen_US
dc.subject.emtreeGeneticsen_US
dc.subject.emtreeMCF-7 cell lineen_US
dc.subject.emtreeMetabolismen_US
dc.subject.emtreeDrug effectsen_US
dc.subject.meshAnalysis of varianceen_US
dc.subject.meshAntigen peptide transporter-1en_US
dc.subject.meshApoptosisen_US
dc.subject.meshCell shapeen_US
dc.subject.meshCell survivalen_US
dc.subject.meshDrug resistance, neoplasmen_US
dc.subject.meshEstradiolen_US
dc.subject.meshGene expression regulation, neoplasticen_US
dc.subject.meshHumansen_US
dc.subject.meshMCF-7 cellsen_US
dc.subject.meshModels, biologicalen_US
dc.subject.meshMultidrug resistance-associated proteinsen_US
dc.subject.meshP-glycoproteinsen_US
dc.subject.meshRNA, messengeren_US
dc.subject.meshTamoxifenen_US
dc.subject.scopusBreast Neoplasms; Aromatase Inhibitors; Estrogen Receptorsen_US
dc.subject.wosCell biologyen_US
dc.subject.wosToxicologyen_US
dc.titleAn in vitro model for the development of acquired tamoxifen resistanceen_US
dc.typeArticle
dc.wos.quartileQ3 (Cell biology)en_US
dc.wos.quartileQ2 (Toxicology)en_US

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