Developmental and molecular correlates of bovine preimplantation embryos

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dc.contributor.authorMisırlıoğlu, Müge
dc.contributor.authorKaya, Abdullah
dc.contributor.authorFirst, Neal L
dc.contributor.authorParrish, John J.
dc.contributor.authorMemili, Erdoğan
dc.contributor.buuauthorSağırkaya, Hakan
dc.contributor.departmentUludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Bölümü.tr_TR
dc.contributor.orcid0000-0002-8335-5645tr_TR
dc.contributor.researcheridAAH-8821-2021tr_TR
dc.contributor.researcheridAAY-8726-2020tr_TR
dc.contributor.scopusid6602400461tr_TR
dc.date.accessioned2021-08-19T13:26:22Z
dc.date.available2021-08-19T13:26:22Z
dc.date.issued2006
dc.description.abstractExpression of embryonic genes is altered in different culture conditions, which influence developmental potential both during preimplantation and fetal development. The objective of this study was to define the effects of culture conditions on: bovine embryonic development to blastocyst stage, blastocyst cell number, apoptosis and expression patterns of a panel of developmentally important genes. Bovine embryos were cultured in vitro in three culture media containing amino acids, namely potassium simplex optimization medium (KSOMaa), Charles Rosenkrans 1 (CR1aa) and synthetic oviductal fluid (SOFaa). Apoptosis in blastocysts was determined by TUNEL assay and expression profiles of developmentally important genes were assayed by real-time PCR. In vivo-produced bovine blastocysts were used as controls for experiments determining gene expression patterns. While the cleavage rates did not differ, embryos cultured in SOFaa had higher rates of development to blastocyst stage (P < 0.05). Mean cell numbers and percentages of apoptotic cells per blastocyst did not differ among the groups. Expression of the heat shock protein 70 (Hsp70) gene was significantly up-regulated in both CR1aa and KSOMaa when compared with SOFaa (P < 0.001). DNA methyltransferase 3a (Dnmt3a) expression was higher in embryos cultured in CR1aa than in those cultured in SOFaa (P < 0.001). Expression of interferon tau (IF-tau) and insulin-like growth factor II receptor (Igf-2r) genes was significantly up-regulated in KSOMaa when compared with CR1aa (P < 0.001). Gene expression did not differ between in vivo-derived blastocysts and their in vitro-derived counterparts. In conclusion, SOFaa supports higher development to blastocyst stage than KSOMaa and CR1aa, and the culture conditions influence gene expression.en_US
dc.identifier.citationSağırkaya, H. vd. (2006). ''Developmental and molecular correlates of bovine preimplantation embryos''. Reproduction, 131(5), 895-904.en_US
dc.identifier.endpage904tr_TR
dc.identifier.issn1470-1626
dc.identifier.issue5tr_TR
dc.identifier.pubmed16672354tr_TR
dc.identifier.scopus2-s2.0-33744486895tr_TR
dc.identifier.startpage895tr_TR
dc.identifier.urihttps://doi.org/10.1530/rep.1.01021
dc.identifier.urihttps://rep.bioscientifica.com/view/journals/rep/131/5/1310895.xml
dc.identifier.urihttp://hdl.handle.net/11452/21481
dc.identifier.volume131tr_TR
dc.identifier.wos000237920300007tr_TR
dc.indexed.pubmedPubmeden_US
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherBioscientificaen_US
dc.relation.collaborationYurt dışıtr_TR
dc.relation.journalReproductionen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectReproductive biologyen_US
dc.subjectDevelopmental biologyen_US
dc.subjectSerumen_US
dc.subjectTranscriptsen_US
dc.subjectFertilizationen_US
dc.subjectNuclear transferen_US
dc.subjectRelative abundanceen_US
dc.subjectMouse embryosen_US
dc.subjectExpressionen_US
dc.subjectIn-vitro cultureen_US
dc.subjectGrowth-factor liganden_US
dc.subjectRNA expressionen_US
dc.subject.scopusBovine Embryo; Blastocyst; Morulaen_US
dc.subject.wosReproductive biologyen_US
dc.subject.wosDevelopmental biologyen_US
dc.titleDevelopmental and molecular correlates of bovine preimplantation embryosen_US
dc.typeArticle
dc.wos.quartileQ2en_US

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