Lack of complete regression of the Day 5 corpus luteum after one or two doses of PGF2α in nonlactating Holstein cows

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dc.contributor.authorNascimento, Aníbal Ballarotti
dc.contributor.authorSouza, Alexandre H.
dc.contributor.authorFilho, Roberto Sartori
dc.contributor.authorWiltbank, Milo Charles
dc.contributor.buuauthorKeskin, Abdülkadir
dc.contributor.departmentUludağ Üniversitesi/Veterinerlik Fakültesi/Klinik Bilimler Bölümü.tr_TR
dc.contributor.researcheridAAH-3831-2021tr_TR
dc.contributor.scopusid24473229800tr_TR
dc.date.accessioned2022-08-22T10:53:44Z
dc.date.available2022-08-22T10:53:44Z
dc.date.issued2014-02-01
dc.description.abstractThe early corpus luteum (CL) (before Day 6) does not regress after a single PGF(2 alpha) treatment. We hypothesized that increasing PGF(2 alpha) dose or number of treatments would allow regression of the early CL (Day 5). Nonlactating Holstein cows (N = 22) were synchronized using the Ovsynch protocol. On Day 5 (Day 0 = second GnRH treatment), cows were assigned to: (1) control (N = 5): no further treatment; (2) 1PGF (N = 6): one dose of 25 mg PGF(2 alpha); (3) 2PGF (N = 5): two doses of 25 mg PGF(2 alpha) (50 mg) given 8 hours apart (second PGF(2 alpha) on Day 5 at the same time as the other PGF(2 alpha) treatments); (4) DPGF (N = 6): double dose of 25 mg PGF(2 alpha) (50 mg) given on Day 5. Blood samples were collected to monitor progesterone (P4) profiles in two periods. In the first period (0 to 24 hours), there were effects of treatment (P = 0.01), time (P < 0.01), and an interaction of treatment and time (P = 0.02). Group 1PGF versus control was different only at 12 hours (P = 0.02). Cows treated with DPGF were different than control at 4 hours (P = 0.04), 12 hours (P < 0.01), and 24 hours (P < 0.01). Only cows treated with 2PGF had lower P4 than control during the entire period and low P4(0.37 +/- 0.17 ng/mL) at 24 hours, usually indicative of luteolysis. In the second period (Day 5 to 15 of the cycle), there were effects of treatment (P < 0.01), time (P < 0.01), and interaction of treatment and time (P = 0.002). Group 1PGF was not different than control from Day 5 to 13 and P4 was greater than control on Day 14 (P = 0.01) and 15 (P < 0.01). Circulating P4 in DPGF cows was lower than control from Day 7 (P = 0.05) through 12 (P < 0.01). Likewise, there were differences between control and 2PGF from Day 7 to 13, but not on Day 14 and 15. On Day 15, all PGF(2 alpha)-treated groups had circulating P4 consistent with an active CL. Ultrasound evaluation confirmed that no CL from any group completely regressed during the experiment and no new ovulations occurred to account for functional CL later in cycle. In summary, a double dose of PGF(2 alpha) (twice on Day 5 or 8 hours apart) can dramatically decrease P4, consistent with classical definitions of luteolysis; however, these CL recover and become fully functional. Thus, the Day 5 CL of mature Holstein cows do not regress even to two doses of PGF(2 alpha).en_US
dc.identifier.citationNascimento, A. B. vd. (2014). "Lack of complete regression of the Day 5 corpus luteum after one or two doses of PGF2α in nonlactating Holstein cows". Theriogenology, 81(3), 389-395.en_US
dc.identifier.endpage395tr_TR
dc.identifier.issn0093-691X
dc.identifier.issn1879-3231
dc.identifier.issue3tr_TR
dc.identifier.pubmed24252637tr_TR
dc.identifier.scopus2-s2.0-84891625406tr_TR
dc.identifier.startpage389tr_TR
dc.identifier.urihttps://doi.org/10.1016/j.theriogenology.2013.10.009
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0093691X13004184
dc.identifier.urihttp://hdl.handle.net/11452/28303
dc.identifier.volume81tr_TR
dc.identifier.wos000330254400003tr_TR
dc.indexed.pubmedPubMeden_US
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherElsevier Scienceen_US
dc.relation.collaborationYurt dışıtr_TR
dc.relation.journalTheriogenologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectProstaglandinen_US
dc.subjectLuteolysisen_US
dc.subjectDairy cowen_US
dc.subjectCorpus luteumen_US
dc.subjectTimed artificial-inseminationen_US
dc.subjectGonadotropin-releasing-hormoneen_US
dc.subjectMessenger-rnaen_US
dc.subjectPregnancy rateen_US
dc.subjectEarly-phaseen_US
dc.subjectBlood-flowen_US
dc.subjectProstaglandin-f2-alphaen_US
dc.subjectSynchronizationen_US
dc.subjectProtocolen_US
dc.subjectExpressionen_US
dc.subjectReproductive biologyen_US
dc.subjectVeterinary sciencesen_US
dc.subjectBosen_US
dc.subjectCorpus luteumen_US
dc.subject.emtreeProstaglandinen_US
dc.subject.emtreeProstaglandin F2 alphaen_US
dc.subject.emtreeAnimalen_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeCattleen_US
dc.subject.emtreeControlled clinical trialen_US
dc.subject.emtreeControlled studyen_US
dc.subject.emtreeCorpus luteumen_US
dc.subject.emtreeDairy cattleen_US
dc.subject.emtreeDrug effecten_US
dc.subject.emtreeEchographyen_US
dc.subject.emtreeEstrus cycleen_US
dc.subject.emtreeFemaleen_US
dc.subject.emtreeLuteolysisen_US
dc.subject.emtreeOvaryen_US
dc.subject.emtreePhysiologyen_US
dc.subject.emtreeRandomized controlled trialen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCattleen_US
dc.subject.meshCorpus luteumen_US
dc.subject.meshDinoprosten_US
dc.subject.meshEstrous cycleen_US
dc.subject.meshEstrus synchronizationen_US
dc.subject.meshFemaleen_US
dc.subject.meshLuteolysisen_US
dc.subject.meshOvaryen_US
dc.subject.scopusArtificial Insemination; Estrus; Cattleen_US
dc.subject.wosReproductive biologyen_US
dc.subject.wosVeterinary sciencesen_US
dc.titleLack of complete regression of the Day 5 corpus luteum after one or two doses of PGF2α in nonlactating Holstein cowsen_US
dc.typeArticle
dc.wos.quartileQ3 (Reproductive biology)en_US
dc.wos.quartileQ1 (Veterinary sciences)en_US

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