Genetic variability of FABP4 c.328 G>A (rs110652478) polmorphism and its association with slaugter weight and carcass traits in aberdeen angus and hereford bulls imported into Turkey
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Date
2021-06-09
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Bursa Uludağ Üniversitesi
Abstract
The study aimed to determine the genotypic distribution of the c.328 G>A (rs110652478) polymorphism in the bovine fatty acid-binding pro tein 4 (FABP4) gene and to determine its effect on slaughter weight in Aberdeen Angus and Hereford bulls imported into Turkey. A total of randomly selected 95 bulls were used in this study. Bulls were housed for fattening in semi-open pens for approximately nine months with the same management procedures. Genotyping of the FABP4 c.328 G>A (rs110652478) polymorphism, located in exon 3, was performed by the PCR-RFLP method. Allele and genotype frequencies were calculated by the standard procedures and the Hardy-Weinberg equilibrium was tested by comparing expected and observed genotype frequencies using chi-square and Fisher’s exact tests. Indices of genetic diversity were calculated based on the allelic frequencies. The least-squares method as applied in a GLM procedure was used to test the association between FABP4 c.328 G>A and phenotypic traits including slaughter weight, hot and cold carcass weight, chilling loss, dressing percentage, and carcass pH. Results revealed that the most frequent genotype was GG (49.18% and 52.94% for Angus and Herefords, respectively) and the minor allele frequency (A allele) was 0.32 in the total population. The genotype distribution was in agreement with Hardy Weinberg equilibrium (P>0.05). The moderately low levels of genetic variability observed in this study may be caused as a result of eventual inbreeding. PIC levels suggest that FABP4 c.328 G>A can be a mildly informative genetic marker for the tested population. Association analysis indicated that the effect of the FABP4 on slaughter weight was statistically significant (P<0.05) in the total population. However, this association was not substantiated in breed-specific analyzes. There was no significant effect of the marker on any remaining phenotypic traits. Consequently, it is important to emphasize that analysis of FABP4 c.328 G>A may provide valuable, but limited, information on imported beef cattle raised in Turkey conditions.
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Keywords
Beef cattle, FABP4, Single nucleotide polymorphism, Marker-assisted selection
Citation
Ardıçlı, S. vd. (2021). ''Genetic variability of FABP4 c.328 G>A (rs110652478) polmorphism and its association with slaugter weight and carcass traits in aberdeen angus and hereford bulls imported into Turkey''. Veteriner Hekimlikte Araştırma Dergisi, 40(1), 10-18.