Tavuklarda Lenfoid Leukozis (LL) hastalığının ELISA (Enzyme Linked Immunosorbent Assay) ve Marek hastalığının (MD) IF (İmmunoflorasan) tekniği ile tanısı

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dc.contributor.authorÖzmen, Özlem
dc.contributor.buuauthorÖzbilgin, Şule
dc.contributor.buuauthorŞen, A.
dc.contributor.buuauthorÇarlı, Kamil Tayfun
dc.contributor.buuauthorSönmez, Gürsel
dc.contributor.departmentUludağ Üniversitesi/Veteriner Fakültesi.tr_TR
dc.contributor.orcid0000-0002-6307-5179tr_TR
dc.contributor.researcheridAAP-7233-2020tr_TR
dc.contributor.researcheridAAG-8117-2021tr_TR
dc.contributor.scopusid57225240619tr_TR
dc.contributor.scopusid7401592869tr_TR
dc.contributor.scopusid56259664500tr_TR
dc.contributor.scopusid6601971539tr_TR
dc.contributor.scopusid55167435000tr_TR
dc.date.accessioned2022-01-10T10:44:56Z
dc.date.available2022-01-10T10:44:56Z
dc.date.issued2001
dc.description.abstractIn this study, our aim was to diagnose lymphoid leukosis and Marek's disease, which are neoplastic diseases of chickens, by ELISA and Indirect Immunofluorescence Test (IIP), respectively. Samples (eggs and tissues) were selectively taken from the flocks suspected of having lymphoid leukosis and Marek's disease. The tissues were macroscopically and microscopically examined. Tissues belonging to some flocks showed typical lesions for lymphoid leukosis and Marek's disease. Seven broiler breede flocks and three commercial layer flocks were tested for avian leukosis virus (ALV) group specific antigen (p27) in the albumen. ALV p27 antigen was detected in five out of ten flocks. The rate of ALV p27 antigen positive albumens among the flocks examined ranged from 10 to 42%. The lymphoid leukosis infection rate was found to be 15.06% (36/239 eggs). All the flocks with lymphoid leukosis were broiler breeders and they were 23-33 weeks old. In the diagnosis of Marek's disease, bone marrow smears were used for the detection of MDV antigen by IIF. Of fifteen flocks examined, twelve were determined to be positive. Fluorescent antigen-positive cells were seen in large numbers and these cells exhibited diffuse cytoplasmic staining with discrete granules. Results showed that smears of bone marrow cells are a suitable test material for a rapid and specific diagnosis of MDV infection in chickens. The chickens infected with MDV were 10-20 weeks old.en_US
dc.identifier.citationÖzbilgin, S. vd. (2001). "Tavuklarda Lenfoid Leukozis (LL) Hastalığının ELISA (Enzyme Linked Immunosorbent Assay) ve Marek Hastalığının (MD) IF (İmmunoflorasan) Tekniği ile Tanısı". Turkish Journal of Veterinary & Animal Science, 25(6), 839-846.tr_TR
dc.identifier.endpage846tr_TR
dc.identifier.issn1300-0128
dc.identifier.issue6tr_TR
dc.identifier.scopus2-s2.0-0035617701tr_TR
dc.identifier.startpage839tr_TR
dc.identifier.urihttps://journals.tubitak.gov.tr/veterinary/issues/vet-01-25-6/vet-25-6-5-9912-2.pdf
dc.identifier.urihttp://hdl.handle.net/11452/23967
dc.identifier.volume25tr_TR
dc.identifier.wos000172885000005tr_TR
dc.indexed.scopusScopusen_US
dc.indexed.trdizinTrDizintr_TR
dc.indexed.wosSCIEen_US
dc.language.isotrtr_TR
dc.publisherTÜBİTAKen_US
dc.relation.collaborationYurt içitr_TR
dc.relation.journalTurkish Journal of Veterinary & Animal Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectChickenen_US
dc.subjectELISAen_US
dc.subjectImmunofluorescenceen_US
dc.subjectLymphoid leukosisen_US
dc.subjectMarek's diseaseen_US
dc.subjectVeterinary sciencesen_US
dc.subjectCongenital transmissionen_US
dc.subjectVirusesen_US
dc.subjectInfectionen_US
dc.subjectHensen_US
dc.subject.scopusAvian Leukosis Virus; Reticuloendotheliosis Virus; P27 Antigenen_US
dc.subject.wosVeterinary sciencesen_US
dc.titleTavuklarda Lenfoid Leukozis (LL) hastalığının ELISA (Enzyme Linked Immunosorbent Assay) ve Marek hastalığının (MD) IF (İmmunoflorasan) tekniği ile tanısıtr_TR
dc.title.alternativeDiagnosis of lymphoid leukosis (LL) by ELISA (enzyme-linked immunosorbent assay) and Marek's disease (MD) by IF (Immunofluorescence) test in chickensen_US
dc.typeArticle
dc.wos.quartileQ4en_US

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