Analysis of ancient DNA from in vitro grown tissues of 1600-year-old seeds revealed the species as Anagyris foetida

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dc.contributor.authorÖzgen, Murat
dc.contributor.authorÖzdilek, Aslı
dc.contributor.authorBirsin, Melahat A.
dc.contributor.authorÖnde, Sertaç
dc.contributor.authorKaya, Zeki
dc.contributor.buuauthorŞahin, Derya
dc.contributor.buuauthorAçıkgöz, Esvet
dc.contributor.departmentUludağ Üniversitesi/Fen-Edebiyat Fakültesi/Arkeoloji Bölümü.tr_TR
dc.contributor.departmentUludağ Üniversitesi/Ziraat Fakültesi/Tarla Bitkileri Bölümü.tr_TR
dc.contributor.scopusid55343714100tr_TR
dc.contributor.scopusid55480264700tr_TR
dc.date.accessioned2022-05-12T07:44:43Z
dc.date.available2022-05-12T07:44:43Z
dc.date.issued2012-12
dc.description.abstractSeven ancient seeds, about 1600 years old, were found during an archaeological excavation in Asar Island which is located in south-western Turkey. These seeds were subjected to germination, in vitro callus induction and molecular characterization experiments to test the viability and plant origin of the seeds. Six of the seven seeds had viable seed components (such as cotyledons) and produced callus tissue in Murashige and Skoog medium supplemented with 2mgl(-1) 6-benzylamino purine (BAP), 0.2 mgl(-1) 1-naphthaleneacetic acid (NAA), 20 gl(-1) sucrose, 2mgl(-1) glycine and 7 gl(-1) agar, but the calli from these seeds failed to yield adventitious shoots. DNA samples from callus tissues produced by ancient seeds in vitro were of good quality. The internal transcribed spacer (ITS) region in nuclear DNA (nDNA) of ancient seeds was amplified successfully. The sequences from amplified ITS DNA products of six ancient seeds indicated that their ITS sequences matched those of Anagyris foetida after subjecting them to BLAST searches in international sequence databases (NCBI). A. foetida is a relict species endemic to the Mediterranean region and used as a herbal medicine. We believe that seed characteristics such as the very hard, extremely smooth and shiny testa, toxic anagyrine alkaloid content and their storage in a pot further improved the longevity of these ancient seeds.en_US
dc.identifier.citationÖzgen, M. vd. (2012). "Analysis of ancient DNA from in vitro grown tissues of 1600-year-old seeds revealed the species as Anagyris foetida". Seed Science Research, 22(4), 279-286.en_US
dc.identifier.endpage286tr_TR
dc.identifier.issn0960-2585
dc.identifier.issn1475-2735
dc.identifier.issue4tr_TR
dc.identifier.scopus2-s2.0-84869043939tr_TR
dc.identifier.startpage279tr_TR
dc.identifier.urihttps://doi.org/10.1017/S0960258512000207
dc.identifier.urihttps://pubag.nal.usda.gov/catalog/5176145
dc.identifier.urihttp://hdl.handle.net/11452/26403
dc.identifier.volume22tr_TR
dc.identifier.wos000310884500006tr_TR
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherCambridge Universityen_US
dc.relation.collaborationYurt içitr_TR
dc.relation.journalSeed Science Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPlant sciencesen_US
dc.subjectAnagyris foetidaen_US
dc.subjectAncient dnaen_US
dc.subjectAncient seedsen_US
dc.subjectInternal transcribed spaceren_US
dc.subjectSeed longevityen_US
dc.subjectSacred lotusen_US
dc.subjectGerminationen_US
dc.subjectL.en_US
dc.subjectHerbariumen_US
dc.subjectGeneticsen_US
dc.subjectSamplesen_US
dc.subjectPlantsen_US
dc.subjectWooden_US
dc.subject.scopusGynandromorphs; Hymenoptera; Mermithoideaen_US
dc.subject.wosPlant sciencesen_US
dc.titleAnalysis of ancient DNA from in vitro grown tissues of 1600-year-old seeds revealed the species as Anagyris foetidaen_US
dc.typeArticle
dc.wos.quartileQ2en_US

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