Civcivlerde deneysel oluşturulan gumboro (IBD) infeksiyonu üzerine vitamin E ve selenyum’un etkisi
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Date
2002-03-07
Authors
Gürel, Aydın
Türkmen, Gülhan
Kuşçu, Burak
Yeşildere, Tahsin
Journal Title
Journal ISSN
Volume Title
Publisher
Uludağ Üniversitesi
Abstract
Bu çalışmada, beş günlükten itibaren yemlerine 200 ppm Vitamin E (Vit E) ve sularına 1,75 mg/l Selenyum (Se) katılmış olan civcivlere, 11. gün IBD virusu (İBDV) verilerek civcivlerde Gumboro hastalığının gelişimi incelendi. Bu amaçla 60 tane günlük SPF civciv kullanıldı. Bu civcivlerin 48 tanesi deney, 12 tanesi kontrol grubunu (E grubu) oluşturdu. Deney grubunu oluşturan civcivler 12’şer civcivden oluşan Vit E + Se+ IBDV(Agrubu), Vit E + IBDV (Bgrubu), Se + IBDV (Cgrubu), IBDV (Dgrubu) olmak üzere dört gruba ayrıldı ve ayrı kümeslere alındı. 5- 17 günler arası ilgili gruplara yukarıda belirtilen dozlarda Vit E ve Se verildi ve kontrol grubu dışındakilere 11. gün IBDV inokule edildi. Deney ve Kontrol gruplarındaki civcivlerden inokulasyon sonrası 9. ve 15. günlerde alınan kan serumlarında IBDV antikor düzeyleri belirlendi. İnokulasyon sonrası 15. gün sakrifiye edilen piliçlerin nekropsisi yapılarak bursa Fabricius lezyonları belirlenip, bursal indeks değerleri tespit edildi. Bursa Fabricius’ta oluşan makroskopik ve mikroskopik lezyonlara ve bursal indeks değerlerine göre, çalışmada A ve B gruplarındaki civcivlerde gelişen Gumboro hastalığı lezyonlarının, diğer gruplardaki civcivlerde gelişen Gumboro lezyonlarına göre daha sınırlı ve hafif olduğu saptandı. Çalışmada bütün gruplarda kan serumlarında saptanan optik dansite değerlerinin birbirine çok yakın olduğu görüldü. Farklı günlerde alınan bu serum antikor düzeyleri artmasına rağmen değerlerinin birbirine yakın bulunması, virusun antijenik özelliğinin zayıf olmasına, veriliş yoluna v.b. bazı kriterlere bağlı olduğu ve bu hayvanlarda humoral bağışıklığın oluşum süresinin tam olarak tamamlanmadığı sonucuna varıldı. Bu nedenle yeme ve suya Vit E ve Se katılmasının IBDV antikor düzeylerine etkisi net olarak saptanamadı. Sonuç olarak, özellikle klinik bulgular, b.Fabricius’ta saptanan makroskobik ve mikroskobik lezyonlara göre A ve B gruplarında Gumboro hastalığı klinik bulgu ve lezyonlarının diğer gruplardakilerine göre daha sınırlı ve hafif geliştiği belirlendi.
The progression of Gumboro disease (IBD) was investigated on chicks which received daily 200 ppm vit E within the feed and 1.75 mg / l Se within drinking water, starting at the age of 5 days and challenged with IBD virus at the age of 11 days.For this purpose, 60 SPF chicks were used. 48 of these comprised the trial group, while the remaining 12 were kept as controls (Group E). The trial group was again divided into 4 subgroups consisting of 12 birds each, and the birds were kept in seperate pens in order to comprise (Group A) Vit E + Se + Gumboro; (Group B) Vit E + Gumboro; (Group C) Se + Gumboro; (Group D) Gumboro trial groups. Between days 5 and 17, the birds were supplemented with Vit. E and Se with the doses as indicated above and all birds except the coltrol group were inoculated with IBD virus at 11 days old. IBDV antibody levels were determined by blood sera samples of the trial and control birds obtained at day 9 and day 15 P.I. At the end of trial period - i.e. 15th day P.I -, necropsies were performed on sacrificed chicks and the lesions on the bursae of Fabricii and Bursal Index values were determined. Samples from the bursas and other organs were collected and sections prepared from the samples were examined under a light microscope after staining with H&E. Considering the macroscopical and microscopical lesions on the bursae of fabricii and the Bursal Index values, lesions in groups A and B were determined to be slighter and more limited than the lesions observed in other groups. In this study, the optical densities determined in the blood sera of all subgroups were found to be very close to each other. Although the sera antibody levels were found to increase within different time intervals, this close relation between different groups were found to have arised from the inadequate time for the birds to develop humoral immunity because of reasons such as weak antigenic properties of the virus, route of inoculation and etc. Thus, it was impossible to interprete the effects of vit E and Se supplementation on IBDV antibody levels, As a result, according to our particular clinical observations and macroscopical or microscopical findings, Gumboro disease related lesions were determined to develop slighter and more limited in groups A and B.
The progression of Gumboro disease (IBD) was investigated on chicks which received daily 200 ppm vit E within the feed and 1.75 mg / l Se within drinking water, starting at the age of 5 days and challenged with IBD virus at the age of 11 days.For this purpose, 60 SPF chicks were used. 48 of these comprised the trial group, while the remaining 12 were kept as controls (Group E). The trial group was again divided into 4 subgroups consisting of 12 birds each, and the birds were kept in seperate pens in order to comprise (Group A) Vit E + Se + Gumboro; (Group B) Vit E + Gumboro; (Group C) Se + Gumboro; (Group D) Gumboro trial groups. Between days 5 and 17, the birds were supplemented with Vit. E and Se with the doses as indicated above and all birds except the coltrol group were inoculated with IBD virus at 11 days old. IBDV antibody levels were determined by blood sera samples of the trial and control birds obtained at day 9 and day 15 P.I. At the end of trial period - i.e. 15th day P.I -, necropsies were performed on sacrificed chicks and the lesions on the bursae of Fabricii and Bursal Index values were determined. Samples from the bursas and other organs were collected and sections prepared from the samples were examined under a light microscope after staining with H&E. Considering the macroscopical and microscopical lesions on the bursae of fabricii and the Bursal Index values, lesions in groups A and B were determined to be slighter and more limited than the lesions observed in other groups. In this study, the optical densities determined in the blood sera of all subgroups were found to be very close to each other. Although the sera antibody levels were found to increase within different time intervals, this close relation between different groups were found to have arised from the inadequate time for the birds to develop humoral immunity because of reasons such as weak antigenic properties of the virus, route of inoculation and etc. Thus, it was impossible to interprete the effects of vit E and Se supplementation on IBDV antibody levels, As a result, according to our particular clinical observations and macroscopical or microscopical findings, Gumboro disease related lesions were determined to develop slighter and more limited in groups A and B.
Description
Keywords
Vitamin E, Selenyum, Gumboro (IBD) hastalığı, Patolojik bulgular, Selenium, Gumboro (IBD) disease, Pathological findings
Citation
Gürel, A vd. (2002). "Civcivlerde deneysel oluşturulan gumboro (IBD) infeksiyonu üzerine vitamin E ve selenyum’un etkisi". Uludağ Üniversitesi Veteriner Fakültesi Dergisi, 22(1-2-3), 13-22.