Changes in serum proteins in dogs with Ehrlichia canis infection

Simple item page
dc.contributor.authorEscribano, Damian
dc.contributor.authorMartinez, Silvia Subiela
dc.contributor.authorCeron, Jose Joaquin
dc.contributor.authorTvarijonaviciute, Asta
dc.contributor.buuauthorCihan, Hüseyin
dc.contributor.buuauthorLevent, Pınar
dc.contributor.buuauthorKocatürk, Meriç
dc.contributor.buuauthorAytuğ, Nilüfer
dc.contributor.buuauthorYılmaz, Zeki
dc.contributor.departmentUludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı.tr_TR
dc.contributor.orcid0000-0001-9836-0749tr_TR
dc.contributor.researcheridABH-3742-2020tr_TR
dc.contributor.researcheridV-5578-2017tr_TR
dc.contributor.scopusid6602381681tr_TR
dc.contributor.scopusid56690616700tr_TR
dc.contributor.scopusid36437200800tr_TR
dc.contributor.scopusid6505835923tr_TR
dc.contributor.scopusid35944810500tr_TR
dc.date.accessioned2023-01-10T07:01:56Z
dc.date.available2023-01-10T07:01:56Z
dc.date.issued2017-10-13
dc.description.abstractThe aim of this study was the identification of proteins differentially represented in the serum proteome of seropositive dogs with (Group 1) and without (Group 2) clinical-pathologic signs consistent with ehrlichiosis compared to healthy control dogs. Serum samples were collected from 20 dogs of various breeds with naturally occurring ehrlichiosis (10 dogs belonged to Group 1 and 10 to Group 2) and 10 healthy dogs. Two-dimensional electrophoresis (2DE) of pooled serum for each of the group of dogs were run in triplicate. 2D image analysis showed 39 spots differently expressed between Group 1 and Group 2 compared with healthy ones. Mass spectrometry analysis allowed identification of 6 proteins: albumin, haptoglobin (Hp), alpha-l-antitrypsin (AAT), Retinol Binding Protein 4 (RBP-4), alpha-l-acid glycoprotein (AGP) and vitamin D-binding protein (VDBP). When a confirmatory study was performed for albumin, Hp, AAT and RBP-4 by using different assays, significant differences (P < 0.05) between diseased and healthy groups were observed. It can be concluded that there are significant changes in the serum proteome of dogs with ehrlichiosis with modifications in proteins related with the acute phase response such as Hp, albumin and AGP, with vitamin A transport such as RBP-4, with inhibitors of serine proteases and anti-inflammatory proteins such as AAT, and vitamin D metabolism and actin scavengers such as VDBP.en_US
dc.description.sponsorship"Ministerio de Economia y Competividad" of Spainen_US
dc.description.sponsorshipFundacion Seneca - 19894/GERM/15en_US
dc.identifier.citationEscribano, D. vd. (2017). ''Changes in serum proteins in dogs with Ehrlichia canis infection''. Microbial Pathogenesis, 113, 34-39.en_US
dc.identifier.endpage39tr_TR
dc.identifier.issn0882-4010
dc.identifier.pubmed29042303tr_TR
dc.identifier.scopus2-s2.0-85032035936tr_TR
dc.identifier.startpage34tr_TR
dc.identifier.urihttps://doi.org/10.1016/j.micpath.2017.10.024
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0882401017308690
dc.identifier.urihttp://hdl.handle.net/11452/30347
dc.identifier.volume113tr_TR
dc.identifier.wos000418631900007tr_TR
dc.indexed.pubmedPubMeden_US
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.collaborationYurt dışıtr_TR
dc.relation.journalMicrobial Pathogenesisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.relation.tubitakTOVAG-213O032en_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectImmunologyen_US
dc.subjectMicrobiologyen_US
dc.subjectBiomarkersen_US
dc.subjectDogsen_US
dc.subjectEhrlichiosisen_US
dc.subjectProteinsen_US
dc.subjectSerumen_US
dc.subjectC-reactive proteinen_US
dc.subjectRetinol-binding-proteinen_US
dc.subjectAcute-phase proteinsen_US
dc.subjectAlpha-1-acid glycoproteinen_US
dc.subjectBeagle dogsen_US
dc.subjectInflammationen_US
dc.subjectExpressionen_US
dc.subjectBiomarkersen_US
dc.subjectDiseaseen_US
dc.subjectDamageen_US
dc.subject.emtreeAlbuminen_US
dc.subject.emtreeAlpha 1 antitrypsinen_US
dc.subject.emtreeHaptoglobinen_US
dc.subject.emtreeOrosomucoiden_US
dc.subject.emtreeRetinol binding protein 4en_US
dc.subject.emtreeVitamin D binding proteinen_US
dc.subject.emtreeAlbuminoiden_US
dc.subject.emtreeAlpha 1 antitrypsinen_US
dc.subject.emtreeHaptoglobinen_US
dc.subject.emtreeOrosomucoiden_US
dc.subject.emtreePlasma proteinen_US
dc.subject.emtreePlasma retinol binding proteinen_US
dc.subject.emtreeProteomeen_US
dc.subject.emtreeVitamin D binding proteinen_US
dc.subject.emtreeAnimal experimenten_US
dc.subject.emtreeAnimal modelen_US
dc.subject.emtreeCanine ehrlichiosisen_US
dc.subject.emtreeClinical featureen_US
dc.subject.emtreeConference paperen_US
dc.subject.emtreeControlled studyen_US
dc.subject.emtreeDogen_US
dc.subject.emtreeEnzyme linked immunosorbent assayen_US
dc.subject.emtreeFemaleen_US
dc.subject.emtreeImage analysisen_US
dc.subject.emtreeMaleen_US
dc.subject.emtreeMass spectrometryen_US
dc.subject.emtreeNonhumanen_US
dc.subject.emtreePriority journalen_US
dc.subject.emtreeProtein blood levelen_US
dc.subject.emtreeProtein determinationen_US
dc.subject.emtreeProtein expressionen_US
dc.subject.emtreeProtein modificationen_US
dc.subject.emtreeProtein transporten_US
dc.subject.emtreeSpectrofluorometryen_US
dc.subject.emtreeTurbidimetryen_US
dc.subject.emtreeTwo dimensional electrophoresisen_US
dc.subject.emtreeVitamin D metabolismen_US
dc.subject.emtreeAnimalen_US
dc.subject.emtreeBlooden_US
dc.subject.emtreeDog diseaseen_US
dc.subject.emtreeEhrlichia canisen_US
dc.subject.emtreeEhrlichiosisen_US
dc.subject.emtreeMetabolismen_US
dc.subject.emtreeMicrobiologyen_US
dc.subject.emtreeVeterinaryen_US
dc.subject.meshAlbuminsen_US
dc.subject.meshAlpha 1-antitrypsinen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBlood proteinsen_US
dc.subject.meshDog diseasesen_US
dc.subject.meshDogsen_US
dc.subject.meshEhrlichia canisen_US
dc.subject.meshEhrlichiosisen_US
dc.subject.meshFemaleen_US
dc.subject.meshHaptoglobinsen_US
dc.subject.meshMaleen_US
dc.subject.meshOrosomucoiden_US
dc.subject.meshProteomeen_US
dc.subject.meshRetinol-binding proteins, plasmaen_US
dc.subject.meshVitamin D-binding proteinen_US
dc.subject.scopusEhrlichia Canis; Animals; Babesia Canis Subsp. Vogelien_US
dc.subject.wosImmunologyen_US
dc.subject.wosMicrobiologyen_US
dc.titleChanges in serum proteins in dogs with Ehrlichia canis infectionen_US
dc.typeArticle
dc.wos.quartileQ3en_US

Files

License bundle
Now showing 1 - 1 of 1
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Web of Science
Scopus