Evaluation of the molecular mechanisms of a palladium(II) saccharinate complex with terpyridine as an anticancer agent

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dc.contributor.authorKaçar, Ömer
dc.contributor.authorAdıgüzel, Zelal
dc.contributor.authorÇetin, Yüksel
dc.contributor.authorTarık, Ahmet Baykal
dc.contributor.authorAçılan, Ceyda
dc.contributor.authorArda, Nazlı
dc.contributor.buuauthorYılmaz, Veysel Turan
dc.contributor.buuauthorUlukaya, Engin
dc.contributor.buuauthorCevatemre, Buse
dc.contributor.departmentUludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü.tr_TR
dc.contributor.departmentUludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.tr_TR
dc.contributor.departmentUludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyokimya Anabilim Dalı.tr_TR
dc.contributor.orcid0000-0002-2849-3332tr_TR
dc.contributor.researcheridK-5792-2018tr_TR
dc.contributor.researcheridL-7238-2018tr_TR
dc.contributor.scopusid7006269202tr_TR
dc.contributor.scopusid55693788600tr_TR
dc.contributor.scopusid6602927353tr_TR
dc.date.accessioned2022-09-19T08:33:47Z
dc.date.available2022-09-19T08:33:47Z
dc.date.issued2014-01
dc.description.abstractMetal-based compounds represent promising anticancer therapeutic agents. In this study, the mechanism of action of a novel metal-based drug, a palladium(II) (Pd) complex ([PdCl(terpy)](sac)2H(2)O, terpy=2,2':6',2 ''-terpyridine and sac=saccharinate), was elucidated. The tested compound induced cytotoxicity in nine different human cancer cell lines that originated from various organs, suggesting a broad spectrum of activity. The IC50 values were significantly higher for noncancerous cells when compared with cancer cells. We found that cells treated with the Pd(II) complex exhibited increased caspase 3/7 activities and condensed/fragmented nuclei, as demonstrated by nuclear staining and DNA laddering. Morphological features, such as cellular shrinkage and blebbing, were also observed, indicating that apoptosis was the primary mechanism of cell death. Pd(II) treatment induced DNA double-stranded breaks both in vitro and in vivo, potentially accounting for the source of stress in these cells. Although caspase 3/7 activities were elevated after Pd(II) treatment, silencing or using inhibitors of caspase 3 did not block apoptosis. Other molecules that could potentially play a role in Pd(II)-induced apoptosis, such as p53 and Bax, were also tested using silencing technology. However, none of these proteins were essential for cell death, indicating either that these molecules do not participate in Pd(II)-induced apoptosis or that other pathways were activated in their absence. Hence, this new molecule might represent a promising anticancer agent that exhibits cytotoxicity in p53-mutant, Bax-mutant, and/or caspase 3-mutant cancer cells.en_US
dc.identifier.citationKaçar, Ö. vd. (2014). "Evaluation of the molecular mechanisms of a palladium(II) saccharinate complex with terpyridine as an anticancer agent". Anti-Cancer Drugs, 25(1), 17-29.en_US
dc.identifier.endpage29tr_TR
dc.identifier.issn0959-4973
dc.identifier.issn1473-5741
dc.identifier.issue1tr_TR
dc.identifier.pubmed24045367tr_TR
dc.identifier.scopus2-s2.0-84889244305tr_TR
dc.identifier.startpage17tr_TR
dc.identifier.urihttps://doi.org/10.1097/CAD.0b013e328364c6ad
dc.identifier.urihttps://journals.lww.com/anti-cancerdrugs/Fulltext/2014/01000/Evaluation_of_the_molecular_mechanisms_of_a.3.aspx
dc.identifier.urihttp://hdl.handle.net/11452/28797
dc.identifier.volume25tr_TR
dc.identifier.wos000327786900003tr_TR
dc.indexed.pubmedPubMeden_US
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherLippincott Williams & Wilkinsen_US
dc.relation.collaborationYurt içitr_TR
dc.relation.collaborationSanayitr_TR
dc.relation.collaborationYurt dışıtr_TR
dc.relation.journalAnti-Cancer Drugsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.relation.tubitakTÜBİTAKtr_TR
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectApoptosisen_US
dc.subjectCanceren_US
dc.subjectCytotoxicityen_US
dc.subjectMetal-based anticancer agentsen_US
dc.subjectPalladium(II) complexen_US
dc.subjectCells in-vitroen_US
dc.subjectDouble-strand breaksen_US
dc.subjectSmooth-muscle-cellsen_US
dc.subjectDNAen_US
dc.subjectPlatinum(II)en_US
dc.subjectCisplatinen_US
dc.subjectDeathen_US
dc.subjectDrugen_US
dc.subjectCytotoxicityen_US
dc.subjectOncologyen_US
dc.subjectPharmacology & pharmacyen_US
dc.subject.emtreeAntineoplastic metal complexen_US
dc.subject.emtreeCaspase 3en_US
dc.subject.emtreeCaspase 7en_US
dc.subject.emtreeGlyceraldehyde 3 phosphate dehydrogenaseen_US
dc.subject.emtreePalladium saccharinate complexen_US
dc.subject.emtreeProtein baxen_US
dc.subject.emtreeProtein p53en_US
dc.subject.emtreeUnclassified drugen_US
dc.subject.emtreeAmpliconen_US
dc.subject.emtreeAnimal cellen_US
dc.subject.emtreeApoptosisen_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeCancer cell cultureen_US
dc.subject.emtreeCell deathen_US
dc.subject.emtreeCell stressen_US
dc.subject.emtreeCell viabilityen_US
dc.subject.emtreeCHO cellen_US
dc.subject.emtreeControlled studyen_US
dc.subject.emtreeCytotoxicityen_US
dc.subject.emtreeDNA fragmentationen_US
dc.subject.emtreeDouble stranded DNA breaken_US
dc.subject.emtreeDrug DNA interactionen_US
dc.subject.emtreeDrug screeningen_US
dc.subject.emtreeEnzyme activityen_US
dc.subject.emtreeFemaleen_US
dc.subject.emtreeGlioblastomaen_US
dc.subject.emtreeHeLa cellen_US
dc.subject.emtreeHumanen_US
dc.subject.emtreeHuman cellen_US
dc.subject.emtreeIC 50en_US
dc.subject.emtreeIn vitro studyen_US
dc.subject.emtreeMaleen_US
dc.subject.emtreeMolecular dynamicsen_US
dc.subject.emtreeNeuroblastoma cellen_US
dc.subject.emtreeNonhumanen_US
dc.subject.emtreePriority journalen_US
dc.subject.emtreeProstate canceren_US
dc.subject.emtreeProtein functionen_US
dc.subject.emtreeReal time polymerase chain reactionen_US
dc.subject.emtreeReverse transcription polymerase chain reactionen_US
dc.subject.emtreeSmooth muscle fiberen_US
dc.subject.emtreeStructure activity relationen_US
dc.subject.meshAntineoplastic agentsen_US
dc.subject.meshApoptosisen_US
dc.subject.meshCell line, tumoren_US
dc.subject.meshCoordination complexesen_US
dc.subject.meshDNA breaks, double-strandeden_US
dc.subject.meshHumansen_US
dc.subject.meshPalladiumen_US
dc.subject.scopusComplex; Palladium; 2-Phenylpyridineen_US
dc.subject.wosOncologyen_US
dc.subject.wosPharmacology & pharmacyen_US
dc.titleEvaluation of the molecular mechanisms of a palladium(II) saccharinate complex with terpyridine as an anticancer agenten_US
dc.typeArticle
dc.wos.quartileQ4 (Oncology)en_US
dc.wos.quartileQ3 (Pharmacology & pharmacy)en_US

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