Expression of matrix metalloproteinase (MMP)-2, MMP-14 and tissue inhibitor of matrix metalloproteinase (TIMP)-2 during bovine placentation and at term with or without placental retention

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dc.contributor.authorDilly, Marc
dc.contributor.authorHambruch, Nina
dc.contributor.authorShenavai, Sima
dc.contributor.authorSchüler, Gerhard
dc.contributor.authorFroehlich, R.
dc.contributor.authorHäeger, Jan Dirk
dc.contributor.authorPfarrer, Christiane D.
dc.contributor.buuauthorÖzalp, Gözde Rabia
dc.contributor.departmentUludağ Üniversitesi/Veterinerlik Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.tr_TR
dc.contributor.orcid0000-0003-4694-6937tr_TR
dc.contributor.researcheridAAE-3607-2019tr_TR
dc.contributor.scopusid23985710500tr_TR
dc.date.accessioned2021-12-15T08:41:44Z
dc.date.available2021-12-15T08:41:44Z
dc.date.issued2011-04-01
dc.description.abstractMatrix metalloproteinases (MMPs) and counteracting tissue inhibitors of metalloproteinases (TIMPs) are balancing extracellular matrix (ECM) formation and degradation. The latter is believed to be an important aspect for the detachment of fetal membranes postpartum when loosening the feto-matemal connection which is a prerequisite to avoid placental retention a common disease in cows leading to considerable economic loss. Membrane-type (MT) MMPs have been suggested as potential activators controlling ECM remodelling. In particular, MT1-MMP (MMP-14) is able to degrade ECM substrates and activate MMP-2 through binding TIMP-2 at the cell surface. Since the connection between the trophoblast and the maternal caruncular epithelium is supported by integrin receptors bound to ECM, we hypothesize that impaired modulation of the ECM by TIMPs/MMPs participates in the aetiology of bovine retained fetal membranes. To analyse this involvement, placentomes were collected from cows after term parturition and timely release of fetal membranes (n = 4) and cows with retained fetal membranes after various treatments for the induction of parturition using progesterone antagonist (aglepristone), PGF(2 alpha) analogue, glucocorticoid, and after elective caesarean sections (each group n = 3). The expression of MMP-14, MMP-2 and of TEMP-2 was examined by real-time-PCR, irnmunohistochemistry, Western blot and zymography. The relative mRNA expression levels of MMP-14 remained unchanged, while the expression levels of TIMP-2 and MMP-2 partly increased in animals with induced parturition and retention of fetal membranes compared to animals without placental retention. MMP-14 protein was expressed in cells of the uninucleated trophoblast, the fetal mesenchyme and maternal stoma. TIMP-2 was present exclusively in trophoblast giant cells, while MMP-2 could be detected in uninucleated trophoblast cells and the fetal mesenchyme. The presence of the activated enzyme was confirmed by zymography. In conclusion, MMP-14, MMP-2 and TIMP-2 are co-localized in the fetal compartment and therefore could influence the timely release of fetal membranes in cattle.en_US
dc.description.sponsorshipGerman Research Foundation (DFG)en_US
dc.identifier.citationDilly, M. vd. (2011). "Expression of matrix metalloproteinase (MMP)-2, MMP-14 and tissue inhibitor of matrix metalloproteinase (TIMP)-2 during bovine placentation and at term with or without placental retention". Theriogenology, 75(6), 1104-1114.en_US
dc.identifier.endpage1114tr_TR
dc.identifier.issn0093-691X
dc.identifier.issn1879-3231
dc.identifier.issue6tr_TR
dc.identifier.pubmed21247626tr_TR
dc.identifier.scopus2-s2.0-79952042979tr_TR
dc.identifier.startpage1104tr_TR
dc.identifier.urihttps://doi.org/10.1016/j.theriogenology.2010.11.019
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0093691X10005984
dc.identifier.urihttp://hdl.handle.net/11452/23269
dc.identifier.volume75tr_TR
dc.identifier.wos000288687500015tr_TR
dc.indexed.pubmedPubmeden_US
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.collaborationYurt dışıtr_TR
dc.relation.journalTheriogenologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.relation.tubitakTÜBİTAKtr_TR
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectReproductive biologyen_US
dc.subjectVeterinary sciencesen_US
dc.subjectAnimaliaen_US
dc.subjectBosen_US
dc.subjectBovinaeen_US
dc.subjectBovine placentaen_US
dc.subjectMatrix metalloproteinasesen_US
dc.subjectRetained fetal membranesen_US
dc.subjectTrophoblast giant-cellsen_US
dc.subjectExtracellular-matrixen_US
dc.subjectGlucocorticoid-receptorsen_US
dc.subjectProgesterone-receptorsen_US
dc.subjectIntegrin receptorsen_US
dc.subjectSheep placentaen_US
dc.subjectMammary-glanden_US
dc.subjectUterine wallen_US
dc.subjectDairy-cattleen_US
dc.subject.emtreeGelatinase Aen_US
dc.subject.emtreeMatrix metalloproteinase 14en_US
dc.subject.emtreeMessenger RNAen_US
dc.subject.emtreeTissue inhibitor of metalloproteinase 2en_US
dc.subject.emtreeAnimalen_US
dc.subject.emtreeAnimal disaseen_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeCattleen_US
dc.subject.emtreeCattle diseaseen_US
dc.subject.emtreeEmbryo membraneen_US
dc.subject.emtreeEnzymologyen_US
dc.subject.emtreeFemaleen_US
dc.subject.emtreeMetabolismen_US
dc.subject.emtreePhysiologyen_US
dc.subject.emtreePlacentaen_US
dc.subject.emtreePregnancyen_US
dc.subject.emtreeReproductionen_US
dc.subject.emtreeRetained placentaen_US
dc.subject.emtreeWestern blottingen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBlotting, westernen_US
dc.subject.meshCattleen_US
dc.subject.meshCattle diseaseen_US
dc.subject.meshExtraembryonic membranesen_US
dc.subject.meshFemaleen_US
dc.subject.meshMatrix metalloproteinase 14en_US
dc.subject.meshMatrix metalloproteinase 2en_US
dc.subject.meshPlacentaen_US
dc.subject.meshPlacenta, retaineden_US
dc.subject.meshPlacentationen_US
dc.subject.meshPregnancyen_US
dc.subject.meshRNA, messengeren_US
dc.subject.meshTissue inhibitor of metalloproteinase-2en_US
dc.subject.scopusEndometritis; Retained Placenta; Dairy Cowsen_US
dc.subject.wosReproductive biologyen_US
dc.subject.wosVeterinary sciencesen_US
dc.titleExpression of matrix metalloproteinase (MMP)-2, MMP-14 and tissue inhibitor of matrix metalloproteinase (TIMP)-2 during bovine placentation and at term with or without placental retentionen_US
dc.typeArticle
dc.wos.quartileQ1 (Veterinary sciences)en_US
dc.wos.quartileQ3 (Reproductive biology)en_US

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