Prevalence, serotype identification by multiplex polymerase chain reaction and antimicrobial resistance patterns of listeria monocytogenes isolated from retail foods

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dc.contributor.buuauthorÇetinkaya, Figen
dc.contributor.buuauthorMuş, Tülay Elal
dc.contributor.buuauthorYıbar, Artun
dc.contributor.buuauthorGüçlü, Nedret
dc.contributor.buuauthorTavşanlı, Hakan
dc.contributor.buuauthorÇıbık, Recep
dc.contributor.departmentUludağ Üniversitesi/Veterinerlik Fakültesi/Gıda Hijyeni ve Teknolojisi Bölümü.tr_TR
dc.contributor.orcid0000-0002-3943-0097tr_TR
dc.contributor.researcheridAAW-5282-2020tr_TR
dc.contributor.researcheridAAI-1993-2021tr_TR
dc.contributor.scopusid8657771200tr_TR
dc.contributor.scopusid55195925900tr_TR
dc.contributor.scopusid38762296900tr_TR
dc.contributor.scopusid56010575700tr_TR
dc.contributor.scopusid56009508000tr_TR
dc.contributor.scopusid56010542400tr_TR
dc.date.accessioned2022-09-06T12:48:09Z
dc.date.available2022-09-06T12:48:09Z
dc.date.issued2014-02
dc.description.abstractIn the present study, a total of 512 food samples composed of raw milk, dairy products, meat/meat products, chicken meat, seafood and raw vegetables were analyzed for the presence of Listeria monocytogenes. The results of the standard identification methods showed that 20 (3.9%) of the analyzed samples were found to harbor this pathogen. Further, 8.4% (13/155) of chicken meats, 0.9% (1/105) of meat/meat products and 13.6% (6/44) of fresh vegetables were contaminated with L.monocytogenes. Interestingly, only 18 of these isolates gave expected band size when they were subjected to molecular confirmation by polymerase chain reaction (PCR). Multiplex PCR serotyping of the strains revealed that 66.6% (12/18) of which belonged to serotype 1/2a (or 3a), 5.6% (1/18) to serotype 1/2b (or 3b, 7), 5.6% (1/18) to serotype 1/2c (or 3c) and 11.1% (2/18) to serotype 4b (or 4d, 4e). Two strains could not be serotyped by multiplex PCR. The strains were also evaluated by disk diffusion assay for their susceptibility to 15 commonly used antimicrobials. Antimicrobial resistance was most frequently observed for clindamycin (94.4%), followed by streptomycin and kanamycin (88.9%); penicillin (72.2%), tetracycline and gentamicin (66.7%); quinopristin/dalfopristin and erythromycin (61.1). Interestingly, 13 strains were resistant to more than five antibiotics. All strains were susceptible to linezolid, teicoplanin and vancomycin. Practical ApplicationsListeria monocytogenes is one of the most important foodborne pathogens responsible for several outbreaks and cases of listeriosis in human. This study focused on the prevalence of L.monocytogenes in different raw and ready-to-eat foodstuffs, and serotype distribution among the isolates. Antibiotic resistance profiles of the isolates were also reported. Information and relief provided to consumers could help elaborate public health and food safety.en_US
dc.identifier.citationÇetinkaya, F. vd. (2014). "Prevalence, serotype identification by multiplex polymerase chain reaction and antimicrobial resistance patterns of listeria monocytogenes isolated from retail foods". Journal of Food Safety, 34(1), 42-49.en_US
dc.identifier.endpage49tr_TR
dc.identifier.issn0149-6085
dc.identifier.issn1745-4565
dc.identifier.issue1tr_TR
dc.identifier.scopus2-s2.0-84894491064tr_TR
dc.identifier.startpage42tr_TR
dc.identifier.urihttps://doi.org/10.1111/jfs.12093
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/full/10.1111/jfs.12093
dc.identifier.urihttp://hdl.handle.net/11452/28503
dc.identifier.volume34tr_TR
dc.identifier.wos000331723700006tr_TR
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.bapV(U)-2009/21tr_TR
dc.relation.journalJournal of Food Safetyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectProcessing plantsen_US
dc.subjectAntibiotic-resistanceen_US
dc.subjectRaw-milken_US
dc.subjectPcren_US
dc.subjectProductsen_US
dc.subjectMeaten_US
dc.subjectPoultryen_US
dc.subjectChickenen_US
dc.subjectIranen_US
dc.subjectSusceptibilityen_US
dc.subjectBiotechnology & applied microbiologyen_US
dc.subjectFood science & technologyen_US
dc.subject.scopusListeriosis; Listeria Monocytogenes; Convenience Fooden_US
dc.subject.wosBiotechnology & applied microbiologyen_US
dc.subject.wosFood science & technologyen_US
dc.titlePrevalence, serotype identification by multiplex polymerase chain reaction and antimicrobial resistance patterns of listeria monocytogenes isolated from retail foodsen_US
dc.typeArticle
dc.wos.quartileQ4 (Biotechnology & applied microbiology)en_US
dc.wos.quartileQ3 (Food science & technology)en_US

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