Enhancement of tendon-bone healing of anterior cruciate ligament grafts by blockage of matrix metalloproteinases

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dc.contributor.buuauthorDemirağ, Burak
dc.contributor.buuauthorSarısözen, Bartu
dc.contributor.buuauthorÖzer, Öner
dc.contributor.buuauthorKaplan, T
dc.contributor.buuauthorÖztürk, Çağatay
dc.contributor.departmentUludağ Üniversitesi/Tıp Fakültesi/Ortopedik Cerrahi Anabilim Dalı.tr_TR
dc.contributor.orcid0000-0003-3133-206Xtr_TR
dc.contributor.researcheridS-6686-2019tr_TR
dc.contributor.researcheridABI-7283-2020tr_TR
dc.date.accessioned2021-06-30T09:52:00Z
dc.date.available2021-06-30T09:52:00Z
dc.date.issued2005-11
dc.description.abstractBackground: The use of soft-tissue grafts for anterior cruciate ligament reconstruction delays the healing process. This delay may be due to biochemical and/or biomechanical insults. We hypothesized that the blocking effect of alpha(2)-macroglobulin on synovial matrix metalloproteinase activity may enhance the healing of tendon graft in a bone tunnel. Methods: The study was performed on twenty-eight healthy, skeletally mature New Zealand White rabbits. Each rabbit underwent bilateral anterior cruciate ligament reconstruction with use of the ipsilateral semitendinosus tendon. Alpha-2-macroglobulin (alpha(2)-macroglobulin) was injected into the knee joint in one limb, and the contralateral limb served as a control. The rabbits were killed two weeks (fourteen rabbits) or five weeks (fourteen rabbits) after the operative procedures. The presence of matrix metalloproteinases in synovial fluid, and the blocking effect of alpha 2-macroglobulin on them, were determined with enzymatic assays. Healing between the tendon and the bone tunnel was assessed morphologically by determining the presence of fibrovascular tissue and collagen fibers. Healing also was assessed quantitatively by measuring the ultimate load to failure of the reconstructed complex. Results: There was an increase in matrix metalloproteinases in the control group; in contrast, there was a decrease in the study group (p < 0.05). In the control specimens, the fibrovascular tissue at the bone-tendon interface had developed into dense connective tissue with poor vascularization. In the treated specimens, the bone tunnel had more areas of denser connective-tissue ingrowth. The interface tissue was more mature and contained numerous perpendicular collagen bundles (Sharpey fibers). The ultimate load to failure was significantly greater in the alpha(2)-macroglobulin-treated specimens than in the untreated controls at both two and five weeks. Conclusions: The present study demonstrated that alpha(2)-macroglobulin blockade of matrix metalloproteinases can enhance bone-tendon healing. This effect of alpha(2)-macroglobulin could occur through its effect solely on collagenase or on a subset of matrix metalloproteinases that are present at the healing interface.en_US
dc.identifier.citationDemirağ, B. vd. (2005). "Enhancement of tendon-bone healing of anterior cruciate ligament grafts by blockage of matrix metalloproteinases". Journal of Bone and Joint Surgery-American Volume, 87A(11), 2401-2410.tr_TR
dc.identifier.endpage2410tr_TR
dc.identifier.issue11tr_TR
dc.identifier.pubmed16264114tr_TR
dc.identifier.scopus2-s2.0-27544462442tr_TR
dc.identifier.startpage2401tr_TR
dc.identifier.urihttps://doi.org/10.2106/JBJS.D.01952
dc.identifier.urihttp://hdl.handle.net/11452/20925
dc.identifier.volume87Atr_TR
dc.identifier.wos000232984500005tr_TR
dc.indexed.pubmedPubmeden_US
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherJournal Bone Joint Surgeryen_US
dc.relation.journalJournal of Bone and Joınt Surgery-Amerıcan Volumeen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectOrthopedicsen_US
dc.subjectSurgeryen_US
dc.subject.wosOrthopedicsen_US
dc.subject.wosSurgeryen_US
dc.titleEnhancement of tendon-bone healing of anterior cruciate ligament grafts by blockage of matrix metalloproteinasesen_US
dc.typeArticle

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