Esophageal muscle cell interaction with biopolymers

dc.contributor.authorKorkmaz, Mevlit
dc.contributor.authorNarcı, Adnan
dc.contributor.authorGüvenç, B. Haluk
dc.contributor.authorYağmurca, Murat
dc.contributor.authorBilir, Ayhan
dc.contributor.buuauthorYakut, Tahsin
dc.contributor.buuauthorGülten, Tuna
dc.contributor.buuauthorYiğit, Barbaros
dc.contributor.departmentUludağ Üniversitesi/Tıp Fakültesi/Tıbbi Genetik Anabilim Dalı.tr_TR
dc.contributor.scopusid6602802424tr_TR
dc.contributor.scopusid6505944216tr_TR
dc.contributor.scopusid55400492100tr_TR
dc.date.accessioned2024-02-29T11:12:56Z
dc.date.available2024-02-29T11:12:56Z
dc.date.issued2007-01-30
dc.description.abstractBackground: The in vitro interactions of esophageal smooth muscle cells (SMCs) with synthetic absorbable polymers were tested and artificial muscle tissues harvested from subcutaneous implantation were examined. Materials/Methods: Esophageal tissue samples from adult and fetal (25-day gestational age) rabbits were cut into small pieces and cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum. Growing cells were identified as SMCs by immunostaining for anti-actin and anti-myosin antibodies. Equal volumes of agar gel and medium were mixed and used for 3-D culture. 5x10(5) cells and 1 mg polyglycolic acid (PGA) and poly-lactide-co-glycolide acid (PLGA) fibers were seeded in six-well tissue culture plates. On days 2 and 7 growing cells were counted by a hemocytometer and cell-polymer interactions were evaluated with light microscopy. Adult and fetal SMCs were seeded onto the PGA and PLGA scaffolds, cultivated for two weeks, and implanted subcutaneously on the backs of the rabbits. Cell-polymer implants were retrieved after four weeks and muscle formation was evaluated histologically and immunohistochemically. Results: Growing cells stained positive for actin and myosin proteins. Cell-polymer interactions were poor after 24 hours, whereas intensive attachment to the fibers was detected 48 hours following cultivation. Both fiber materials supported cell proliferation. PLGA scaffolds improved muscle formation more efficiently than PGA, and fetal and adult SMCs showed similar mass quality. Conclusions: Scaffolds are important as cell-carrying vehicles, and material-cell interactions should be tested before application. A 3-D culture prepared with agar gel and medium is practical for testing material toxicity.en_US
dc.identifier.citationKorkmaz, M. vd. (2006). "Esophageal muscle cell interaction with biopolymers". Medical Science Monitor, 13(2), BR46-BR49.en_US
dc.identifier.endpageBR49tr_TR
dc.identifier.issn1234-1010
dc.identifier.issue2tr_TR
dc.identifier.pubmed17261980tr_TR
dc.identifier.scopus2-s2.0-33846910897tr_TR
dc.identifier.startpageBR46tr_TR
dc.identifier.urihttps://hdl.handle.net/11452/40086en_US
dc.identifier.volume13tr_TR
dc.identifier.wos000244491200004tr_TR
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherInternational Scientific Literatureen_US
dc.relation.collaborationYurt içitr_TR
dc.relation.journalMedical Science Monitoren_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectEsophagusen_US
dc.subjectCell cultureen_US
dc.subjectSmooth muscle cellen_US
dc.subjectTissuesen_US
dc.subjectCaffoldsen_US
dc.subjectCultureen_US
dc.subjectResearch & experimental medicineen_US
dc.subject.emtreeActinen_US
dc.subject.emtreeActin antibodyen_US
dc.subject.emtreeBiopolymeren_US
dc.subject.emtreeMyosinen_US
dc.subject.emtreeMyosin antibodyen_US
dc.subject.emtreePolyglactinen_US
dc.subject.emtreePolyglycolic aciden_US
dc.subject.emtreeAgar mediumen_US
dc.subject.emtreeAnimal cellen_US
dc.subject.emtreeAnimal tissueen_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeCell proliferationen_US
dc.subject.emtreeControlled studyen_US
dc.subject.emtreeEsophagusen_US
dc.subject.emtreeFetusen_US
dc.subject.emtreeGestational ageen_US
dc.subject.emtreeHistologyen_US
dc.subject.emtreeImmunohistochemistryen_US
dc.subject.emtreeMicroscopyen_US
dc.subject.emtreeMuscle cellen_US
dc.subject.emtreeNonhumanen_US
dc.subject.emtreeRabbiten_US
dc.subject.emtreeTissue cultureen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBiopolymersen_US
dc.subject.meshEsophagusen_US
dc.subject.meshLactic aciden_US
dc.subject.meshMyocytes, smooth muscleen_US
dc.subject.meshPolyglycolic aciden_US
dc.subject.meshPolymersen_US
dc.subject.meshRabbitsen_US
dc.subject.meshTissue engineeringen_US
dc.subject.scopusBladder; Tissue Engineering; Urotheliumen_US
dc.subject.wosMedicine, research & experimentalen_US
dc.titleEsophageal muscle cell interaction with biopolymersen_US
dc.typeArticleen_US
dc.wos.quartileQ3 (Medicine, research & experimental)en_US

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