Investigation of diagnostic importance of platelet closure times measured by Platelet Function Analyzer-PFA 100 in dogs with endotoxemia

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dc.contributor.buuauthorYılmaz, Zeki
dc.contributor.buuauthorİlçöl, Yeşim Özarda
dc.contributor.buuauthorUlus, İsmail Hakkı
dc.contributor.departmentUludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı.tr_TR
dc.contributor.departmentUludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.tr_TR
dc.contributor.departmentUludağ Üniversitesi/Tıp Fakültesi/Farmakoloji ve Klinik Eczacılık Anabilim Dalı.tr_TR
dc.contributor.departmentUludağ Üniversitesi/Veteriner Fakültesi Klinikleri.tr_TR
dc.contributor.orcid0000-0001-9836-0749tr_TR
dc.contributor.researcheridA-9637-2008tr_TR
dc.contributor.researcheridAAL-8873-2021tr_TR
dc.contributor.researcheridD-5340-2015tr_TR
dc.contributor.scopusid35944810500tr_TR
dc.contributor.scopusid55665181500tr_TR
dc.contributor.scopusid7004271086tr_TR
dc.date.accessioned2021-09-15T07:52:32Z
dc.date.available2021-09-15T07:52:32Z
dc.date.issued2005-07
dc.description.abstractThis study was performed to evaluate the diagnostic importance of the platelet closure times measured by the Platelet Function Analyzer (PFA-100 (TM)) in dogs with endotoxemia. E.coli endotoxin was given intravenously once, at the dose of 0.02 mg/kg or I mg/kg in groups I (n=9) and 11 (n=8), respectively. Normal saline (0.1 ml/kg) was injected in group III (n=8).The dogs were monitored for 48 In, and venous blood samples were collected prior to (baseline) and at intervals of 0.5, 1, 2,4,6,8,12,24 and 48 h subsequent to the treatments. The white blood cell (WBC), platelet counts, and hematocrit (Hct) values were recorded. Platelet closure times were determined, using collagen/epinephrine (CEPI) and collagen/adenosine diphosphate (CADP) cartridges. Within 0.5 h after the endotoxin application baseline WBC and platelet counts (mean +/- SD) decreased significantly (p < 0.001) to 2000 +/- 500 and 1850 +/- 200 cells/mu l or 69.000 +/- 12.500 and 27.000 +/- 6.400 cells/mu l in groups I and 11, respectively. Platelet counts remained low during the first 1-48 h, but the WBC count was high at the 8(th)-48(th) h, in groups I and II, compared with baselines (p < 0.001). After the application of the endotoxin, Hct values increased from baseline values of 37 +/- 3 or 39 +/- 2 % to 48 +/- 2 or 51 +/- 3 %,within I h (p < 0.001), in groups I and 11, respectively. Hct values in group 11 were notably higher (p < 0.001) than those of group I during the 2(nd)-48(th) h. Hematological parameters and closure times did not differ significantly throughout the study in group III. Baseline closure time ranged from 79 +/- 5 seconds (s) to 86 +/- 5 s for CADP and 144 +/- 13 s to 159 +/- 14 s for CEPI in all dogs (n=25). At 0.5 h after the endotoxin, the closure times of CADP as well as CEPI declined to 62 +/- 6 s and 76 +/- 8 s in group I (p < 0.001) and 57 +/- 5 s and 75 +/- 6 s in group 11 (p < 0.001). Afterwards, closure time prolonged to the levels of 280 8 s (CADP) and 294 5 s (CEPI) by 48 h (p < 0.001) in group 11, but returned to the baseline limit in group I. In conclusion, our results show that the shortened closure times may serve as a very early diagnostic sign of endotoxemia, prolonged closure times however may be used as an index for the severity of endotoxemia.en_US
dc.identifier.citationYılmaz, Z. vd. (2005). "Investigation of diagnostic importance of platelet closure times measured by Platelet Function Analyzer-PFA 100 in dogs with endotoxemia". Berliner Und Munchener Tierarztliche Wochenschrift, 118(7-8), 341-348.en_US
dc.identifier.endpage348tr_TR
dc.identifier.issn0005-9366
dc.identifier.issue7-8tr_TR
dc.identifier.pubmed16048047tr_TR
dc.identifier.scopus2-s2.0-23044457952tr_TR
dc.identifier.startpage341tr_TR
dc.identifier.urihttp://hdl.handle.net/11452/21963
dc.identifier.volume118tr_TR
dc.identifier.wos000230498700013tr_TR
dc.indexed.pubmedPubmeden_US
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherSchluetersche Verlagsgesellschaft Mbh & Co Kgen_US
dc.relation.journalBerliner und Munchener Tierarzliche Wochenschriftde
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPlateleten_US
dc.subjectClosure timeen_US
dc.subjectPFA-100en_US
dc.subjectEndotoxinen_US
dc.subjectDogen_US
dc.subjectDisseminated intravascular coagulationen_US
dc.subjectOf-care instrumenten_US
dc.subjectCanine endotoxemiaen_US
dc.subjectSystemic inflammationen_US
dc.subjectPrimary hemostasisen_US
dc.subjectWhole-blooden_US
dc.subjectAggregationen_US
dc.subjectDisordersen_US
dc.subjectMediatorsen_US
dc.subjectVeterinary sciencesen_US
dc.subjectCanis familiarisen_US
dc.subjectEscherichia colien_US
dc.subject.emtreeLipopolysaccharideen_US
dc.subject.emtreeAnimalen_US
dc.subject.emtreeAnimal diseaseen_US
dc.subject.emtreeAutoanalysisen_US
dc.subject.emtreeBlooden_US
dc.subject.emtreeDogen_US
dc.subject.emtreeDog diseaseen_US
dc.subject.emtreeEndotoxemiaen_US
dc.subject.emtreeEscherichia colien_US
dc.subject.emtreeFemaleen_US
dc.subject.emtreeMaleen_US
dc.subject.emtreePhysiologyen_US
dc.subject.emtreeThrombocyte aggregationen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAutoanalysisen_US
dc.subject.meshDog diseasesen_US
dc.subject.meshDogsen_US
dc.subject.meshEndotoxemiaen_US
dc.subject.meshEscherichia colien_US
dc.subject.meshFemaleen_US
dc.subject.meshLipopolysaccharidesen_US
dc.subject.meshMaleen_US
dc.subject.meshPlateleten_US
dc.subject.meshAggregationen_US
dc.subject.wosVeterinary sciencesen_US
dc.titleInvestigation of diagnostic importance of platelet closure times measured by Platelet Function Analyzer-PFA 100 in dogs with endotoxemiaen_US
dc.typeArticle
dc.wos.quartileQ2en_US

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