Bombus terrestris sindirim kanalının bazı bölge mukozalarının klasik ve lektin histokimyasal yapısı
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Date
2016-12-12
Authors
Göçer, Nazan
Çınar, Kenan
Journal Title
Journal ISSN
Volume Title
Publisher
Uludağ Üniversitesi
Abstract
Bu çalışmada 15 adet Bombus terrestris türü arıdan Proventrikulus, Krop, Ventrikulus ve İleumdan alınan örnekler materyal olarak kullanıldı. Alınan örneklere ait kesitlere UEA-I, Con-A, PNA, SBA ve WGA lektinleri uygulandı. Yapılan incelemeler sonucunda Ventrikulus ve ileumda UEA-I reaksiyonun örtü epiteli yüzeyinde güçlü olduğu belirlendi. Kropta ise reaksiyona rastlanmadı. Ventrikulus ve ileumda örtü epiteli yüzeyi ile sitoplazmanın SBA ile çok güçlü reaksiyon verdiği gözlendi. WGA uygulaması sonucunda proventrikulusun örtü epitelinde çok güçlü reaksiyon belirlendi. PNA reaksiyonunun da proventrikulus ve krop örtü epitelinde güçlü ya da çok güçlü olduğu belirlendi. ConA uygulamasında ise krop hücre yüzeyi örtü epitelinde reaksiyon gözlenmedi.
In this study, proventriculus, crop, ventriculus and ileum samples taken from 15 bees belonging to the species Bombusterrestris were used as material. UEA-I, Con-A, PNA, SBA and WGA lectins were applied to sections. As a result of evaluation of sections to which lectin histochemistry was applied, strong UEA-I reaction was observed on the epithelial surface of ventriculus and ileum. Any reaction to UEA-I was not shown in the crop region. Very strong reaction to SBA was detected at the epithelial surface and cytoplasm of the ventriculus and ileum. Epithelial surface of proventriculus stained with WGA very strongly. Strong or very strong reaction to PNA was observed in the epithelial surface of proventriculus and crop. Cell surface of crop did not react with ConA.
In this study, proventriculus, crop, ventriculus and ileum samples taken from 15 bees belonging to the species Bombusterrestris were used as material. UEA-I, Con-A, PNA, SBA and WGA lectins were applied to sections. As a result of evaluation of sections to which lectin histochemistry was applied, strong UEA-I reaction was observed on the epithelial surface of ventriculus and ileum. Any reaction to UEA-I was not shown in the crop region. Very strong reaction to SBA was detected at the epithelial surface and cytoplasm of the ventriculus and ileum. Epithelial surface of proventriculus stained with WGA very strongly. Strong or very strong reaction to PNA was observed in the epithelial surface of proventriculus and crop. Cell surface of crop did not react with ConA.
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Keywords
Bombus terrestris, Sindirim kanalı mukozası, Lektin histokimyasal yapı, Digestive tract mucosa, Lectin histochemical structure