Associations between GH, PRL, STAT5A, OPN, PIT-1, LEP and FGF2 polymorphisms and fertility in holstein-friesian heifers
dc.contributor.author | Yılmaz, Onur | |
dc.contributor.author | Okut, Hayrettin | |
dc.contributor.author | Ata, Nezih | |
dc.contributor.buuauthor | Öner, Yasemin | |
dc.contributor.buuauthor | Yılmazbaş, Mecitoğlu Gülnaz | |
dc.contributor.buuauthor | Keskin, Abdulkadir | |
dc.contributor.department | Uludağ Üniversitesi/Ziraat Fakültesi/Zooteknik, Biyometri ve Genetik Bölümü. | tr_TR |
dc.contributor.department | Uludağ Üniversitesi/Veteriner Fakültesi. | tr_TR |
dc.contributor.orcid | 0000-0002-2904-8986 | tr_TR |
dc.contributor.orcid | 0000-0003-2817-3221 | tr_TR |
dc.contributor.researcherid | ABB-3181-2020 | tr_TR |
dc.contributor.researcherid | AAG-4440-2019 | tr_TR |
dc.contributor.scopusid | 9634793800 | tr_TR |
dc.contributor.scopusid | 36457964000 | tr_TR |
dc.contributor.scopusid | 24473229800 | tr_TR |
dc.date.accessioned | 2023-01-09T06:53:46Z | |
dc.date.available | 2023-01-09T06:53:46Z | |
dc.date.issued | 2017 | |
dc.description.abstract | In this study, it was aimed to investigate polymorphisms in seven genes (GH, PRL, STAT5A, OPN, PIT-1, LEP and FGF2) related to reproductive traits in dairy heifers. Frequency distributions of the genotypes between fertile and repeat breeder heifers groups were investigated. Allele effects on fertility were also analyzed. Blood samples were taken from a total of 160 Holstein-Friesian heifers and they were divided into two groups according to their artificial insemination numbers (AI). The heifers becoming pregnant after the first AI were used as the fertile heifers (FH, n= 80) and the heifers with 3 or more equal AIs were accepted as the repeat breeder heifers (RBH, n= 80). All the animals were genotyped by the PCR-RFLP method for seven genes and the association works were performed for 145 animals (RBH, n= 79; FH n= 66). For all loci investigated, two alleles and three genotypes were found for overall population with the exception that PRL locus had two alleles and two genotypes. The chi-square test (.2) revealed that the whole population and the two groups separately were at Hardy-Weinberg equilibrium. The genotype distributions of PIT-1 and STAT5A conspicuously differed between the FH and the RBH groups; however, these differences were not found significant. Association of GH-AB genotype was found significant on AI number for the first pregnancy. Mixed effect logistic regression model was used to investigate the allele effects on fertility. No linkage disequilibrium was detected between the investigated loci. | en_US |
dc.description.abstract | Bu çalışmada sütçü düvelerde reprodüktif özellikler ile ilişkili yedi gendeki (GH, PRL, STAT5A, OPN, PIT-1, LEP ve FGF2) polimorfizimlerin araştırılması amaçlanmıştır. Genotip frekanslarının fertil ve repeat breeder düve gruplarındaki dağılımı araştırılmıştır. Ayrıca fertilite üzerine allel etkisi de incelenmiştir. Toplam 160 Holstein-Friesian düveden kan alınmış ve bu düveler tohumlama sayılarına (ST) göre iki gruba ayrılmıştır. İlk tohumlamada gebe kalan düveler fertil düve (FH, n=80) olarak kullanılmış ve üç veya daha fazla ST’si olan düveler repeat breeder düve (RBH, n=80) olarak kabul edilmiştir. Tüm hayvanlar yedi gen bakımından PCR-RFLP metodu ile genotiplendirilmiş ve ilişkilendirme çalışmaları toplam 145 hayvanda yapılmıştır (RBH, n=79; FH, n=66). İki allel ve iki genotipin bulunduğu PRL hariç incelenen tüm lokuslarda iki allel ve üç genotip belirlenmiştir. Ki-kare sonuçları (χ2) tüm populasyonun ve ayrı ayrı grupların Hardy-Weinberg dengesinde olduğunu ortaya koymuştur. PIT-1 ve STAT5A lokuslarının genotip frekanslarının dağılımları FH ve RBH grupları arasında belirgin biçimde farklı olmasına rağmen bu farklılık istatistiksel anlamda önemli bulunmamıştır. GH-AB genotipinin fertilite üzerine etkisi önemli bulunmuştur. Fertilite üzerindeki allel etkisini incelemek için karışık etkili lojistik regresyon analizi kullanılmıştır. İncelenen lokuslar arasında bir bağlantı dengesizliği belirlenmemiştir. | tr_TR |
dc.description.sponsorship | American Journal Experts (AJE). | en_US |
dc.identifier.citation | Öner, Y. vd. (2017). ''Associations between GH, PRL, STAT5A, OPN, PIT-1, LEP and FGF2 polymorphisms and fertility in holstein-friesian heifers''. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 23(4), 527-534. | tr_TR |
dc.identifier.endpage | 534 | tr_TR |
dc.identifier.issn | 1300-6045 | |
dc.identifier.issue | 4 | tr_TR |
dc.identifier.scopus | 2-s2.0-85018303446 | tr_TR |
dc.identifier.startpage | 527 | tr_TR |
dc.identifier.uri | https://doi.org/10.9775/kvfd.2016.17192 | |
dc.identifier.uri | http://hdl.handle.net/11452/30322 | |
dc.identifier.volume | 23 | tr_TR |
dc.identifier.wos | 000401789000003 | tr_TR |
dc.indexed.scopus | Scopus | en_US |
dc.indexed.trdizin | TrDizin | tr_TR |
dc.indexed.wos | SCIE | en_US |
dc.language.iso | en | en_US |
dc.publisher | Kafkas Üniversitesi Veteriner Fakültesi Dergisi | tr_TR |
dc.relation.bap | KUAP 2013/49 | tr_TR |
dc.relation.collaboration | Yurt içi | tr_TR |
dc.relation.collaboration | Yurt dışı | tr_TR |
dc.relation.journal | Kafkas Üniversitesi Veteriner Fakültesi Dergisi | tr_TR |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | tr_TR |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Veterinary sciences | en_US |
dc.subject | Dairy heifers | en_US |
dc.subject | Fertility | en_US |
dc.subject | Infertility | en_US |
dc.subject | Polymorphism | en_US |
dc.subject | Growth-hormone gene | en_US |
dc.subject | Milk-production traits | en_US |
dc.subject | Embryonic survival | en_US |
dc.subject | Dairy-cattle | en_US |
dc.subject | Bovine prolactin | en_US |
dc.subject | Interferon-tau | en_US |
dc.subject | Pou1F1 gene | en_US |
dc.subject | Brown swiss | en_US |
dc.subject | Intron 3 | en_US |
dc.subject | Protein | en_US |
dc.subject | Polimorfizm | tr_TR |
dc.subject | Fertilite | tr_TR |
dc.subject | İnfertilite | tr_TR |
dc.subject | Sütçü düve | tr_TR |
dc.subject.emtree | Fibroblast growth factor 2 | en_US |
dc.subject.emtree | STAT5a protein | en_US |
dc.subject.emtree | Transcription factor pit 1 | en_US |
dc.subject.emtree | Gene frequency | en_US |
dc.subject.emtree | Gene linkage disequilibrium | en_US |
dc.subject.emtree | Genetic association | en_US |
dc.subject.emtree | Genetic parameters | en_US |
dc.subject.emtree | Genetic variability | en_US |
dc.subject.emtree | Genotype | en_US |
dc.subject.emtree | Genotype frequency | en_US |
dc.subject.emtree | GH gene | en_US |
dc.subject.emtree | Heterozygosity | en_US |
dc.subject.emtree | Holstein-friesian cattle | en_US |
dc.subject.emtree | LEP gene | en_US |
dc.subject.emtree | Nonhuman | en_US |
dc.subject.emtree | OPN gene | en_US |
dc.subject.emtree | PIT 1 gene | en_US |
dc.subject.emtree | Polymerase chain reaction | en_US |
dc.subject.emtree | PRL gene | en_US |
dc.subject.emtree | Restriction fragment length polymorphism | en_US |
dc.subject.emtree | STAT5A gene | en_US |
dc.subject.emtree | Age | en_US |
dc.subject.emtree | Animal experiment | en_US |
dc.subject.emtree | Animal genetics | en_US |
dc.subject.emtree | Article | en_US |
dc.subject.emtree | Cattle breed | en_US |
dc.subject.emtree | Controlled study | en_US |
dc.subject.emtree | DNA polymorphism | en_US |
dc.subject.emtree | Fertility | en_US |
dc.subject.emtree | FGF2 gene | en_US |
dc.subject.emtree | Gene | en_US |
dc.subject.scopus | Growth Hormone; Gene; Somatotropin Receptors | en_US |
dc.subject.wos | Veterinary sciences | en_US |
dc.title | Associations between GH, PRL, STAT5A, OPN, PIT-1, LEP and FGF2 polymorphisms and fertility in holstein-friesian heifers | en_US |
dc.title.alternative | Holstein-Friesian düvelerde fertilite ile GH, PRL, STAT5A, OPN, PIT-1, LEP ve FGF2 polimorfizimlerinin ilişkileri | tr_TR |
dc.type | Article | |
dc.wos.quartile | Q2 | en_US |