Associations between GH, PRL, STAT5A, OPN, PIT-1, LEP and FGF2 polymorphisms and fertility in holstein-friesian heifers

dc.contributor.authorYılmaz, Onur
dc.contributor.authorOkut, Hayrettin
dc.contributor.authorAta, Nezih
dc.contributor.buuauthorÖner, Yasemin
dc.contributor.buuauthorYılmazbaş, Mecitoğlu Gülnaz
dc.contributor.buuauthorKeskin, Abdulkadir
dc.contributor.departmentUludağ Üniversitesi/Ziraat Fakültesi/Zooteknik, Biyometri ve Genetik Bölümü.tr_TR
dc.contributor.departmentUludağ Üniversitesi/Veteriner Fakültesi.tr_TR
dc.contributor.orcid0000-0002-2904-8986tr_TR
dc.contributor.orcid0000-0003-2817-3221tr_TR
dc.contributor.researcheridABB-3181-2020tr_TR
dc.contributor.researcheridAAG-4440-2019tr_TR
dc.contributor.scopusid9634793800tr_TR
dc.contributor.scopusid36457964000tr_TR
dc.contributor.scopusid24473229800tr_TR
dc.date.accessioned2023-01-09T06:53:46Z
dc.date.available2023-01-09T06:53:46Z
dc.date.issued2017
dc.description.abstractIn this study, it was aimed to investigate polymorphisms in seven genes (GH, PRL, STAT5A, OPN, PIT-1, LEP and FGF2) related to reproductive traits in dairy heifers. Frequency distributions of the genotypes between fertile and repeat breeder heifers groups were investigated. Allele effects on fertility were also analyzed. Blood samples were taken from a total of 160 Holstein-Friesian heifers and they were divided into two groups according to their artificial insemination numbers (AI). The heifers becoming pregnant after the first AI were used as the fertile heifers (FH, n= 80) and the heifers with 3 or more equal AIs were accepted as the repeat breeder heifers (RBH, n= 80). All the animals were genotyped by the PCR-RFLP method for seven genes and the association works were performed for 145 animals (RBH, n= 79; FH n= 66). For all loci investigated, two alleles and three genotypes were found for overall population with the exception that PRL locus had two alleles and two genotypes. The chi-square test (.2) revealed that the whole population and the two groups separately were at Hardy-Weinberg equilibrium. The genotype distributions of PIT-1 and STAT5A conspicuously differed between the FH and the RBH groups; however, these differences were not found significant. Association of GH-AB genotype was found significant on AI number for the first pregnancy. Mixed effect logistic regression model was used to investigate the allele effects on fertility. No linkage disequilibrium was detected between the investigated loci.en_US
dc.description.abstractBu çalışmada sütçü düvelerde reprodüktif özellikler ile ilişkili yedi gendeki (GH, PRL, STAT5A, OPN, PIT-1, LEP ve FGF2) polimorfizimlerin araştırılması amaçlanmıştır. Genotip frekanslarının fertil ve repeat breeder düve gruplarındaki dağılımı araştırılmıştır. Ayrıca fertilite üzerine allel etkisi de incelenmiştir. Toplam 160 Holstein-Friesian düveden kan alınmış ve bu düveler tohumlama sayılarına (ST) göre iki gruba ayrılmıştır. İlk tohumlamada gebe kalan düveler fertil düve (FH, n=80) olarak kullanılmış ve üç veya daha fazla ST’si olan düveler repeat breeder düve (RBH, n=80) olarak kabul edilmiştir. Tüm hayvanlar yedi gen bakımından PCR-RFLP metodu ile genotiplendirilmiş ve ilişkilendirme çalışmaları toplam 145 hayvanda yapılmıştır (RBH, n=79; FH, n=66). İki allel ve iki genotipin bulunduğu PRL hariç incelenen tüm lokuslarda iki allel ve üç genotip belirlenmiştir. Ki-kare sonuçları (χ2) tüm populasyonun ve ayrı ayrı grupların Hardy-Weinberg dengesinde olduğunu ortaya koymuştur. PIT-1 ve STAT5A lokuslarının genotip frekanslarının dağılımları FH ve RBH grupları arasında belirgin biçimde farklı olmasına rağmen bu farklılık istatistiksel anlamda önemli bulunmamıştır. GH-AB genotipinin fertilite üzerine etkisi önemli bulunmuştur. Fertilite üzerindeki allel etkisini incelemek için karışık etkili lojistik regresyon analizi kullanılmıştır. İncelenen lokuslar arasında bir bağlantı dengesizliği belirlenmemiştir.tr_TR
dc.description.sponsorshipAmerican Journal Experts (AJE).en_US
dc.identifier.citationÖner, Y. vd. (2017). ''Associations between GH, PRL, STAT5A, OPN, PIT-1, LEP and FGF2 polymorphisms and fertility in holstein-friesian heifers''. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 23(4), 527-534.tr_TR
dc.identifier.endpage534tr_TR
dc.identifier.issn1300-6045
dc.identifier.issue4tr_TR
dc.identifier.scopus2-s2.0-85018303446tr_TR
dc.identifier.startpage527tr_TR
dc.identifier.urihttps://doi.org/10.9775/kvfd.2016.17192
dc.identifier.urihttp://hdl.handle.net/11452/30322
dc.identifier.volume23tr_TR
dc.identifier.wos000401789000003tr_TR
dc.indexed.scopusScopusen_US
dc.indexed.trdizinTrDizintr_TR
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherKafkas Üniversitesi Veteriner Fakültesi Dergisitr_TR
dc.relation.bapKUAP 2013/49tr_TR
dc.relation.collaborationYurt içitr_TR
dc.relation.collaborationYurt dışıtr_TR
dc.relation.journalKafkas Üniversitesi Veteriner Fakültesi Dergisitr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectVeterinary sciencesen_US
dc.subjectDairy heifersen_US
dc.subjectFertilityen_US
dc.subjectInfertilityen_US
dc.subjectPolymorphismen_US
dc.subjectGrowth-hormone geneen_US
dc.subjectMilk-production traitsen_US
dc.subjectEmbryonic survivalen_US
dc.subjectDairy-cattleen_US
dc.subjectBovine prolactinen_US
dc.subjectInterferon-tauen_US
dc.subjectPou1F1 geneen_US
dc.subjectBrown swissen_US
dc.subjectIntron 3en_US
dc.subjectProteinen_US
dc.subjectPolimorfizmtr_TR
dc.subjectFertilitetr_TR
dc.subjectİnfertilitetr_TR
dc.subjectSütçü düvetr_TR
dc.subject.emtreeFibroblast growth factor 2en_US
dc.subject.emtreeSTAT5a proteinen_US
dc.subject.emtreeTranscription factor pit 1en_US
dc.subject.emtreeGene frequencyen_US
dc.subject.emtreeGene linkage disequilibriumen_US
dc.subject.emtreeGenetic associationen_US
dc.subject.emtreeGenetic parametersen_US
dc.subject.emtreeGenetic variabilityen_US
dc.subject.emtreeGenotypeen_US
dc.subject.emtreeGenotype frequencyen_US
dc.subject.emtreeGH geneen_US
dc.subject.emtreeHeterozygosityen_US
dc.subject.emtreeHolstein-friesian cattleen_US
dc.subject.emtreeLEP geneen_US
dc.subject.emtreeNonhumanen_US
dc.subject.emtreeOPN geneen_US
dc.subject.emtreePIT 1 geneen_US
dc.subject.emtreePolymerase chain reactionen_US
dc.subject.emtreePRL geneen_US
dc.subject.emtreeRestriction fragment length polymorphismen_US
dc.subject.emtreeSTAT5A geneen_US
dc.subject.emtreeAgeen_US
dc.subject.emtreeAnimal experimenten_US
dc.subject.emtreeAnimal geneticsen_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeCattle breeden_US
dc.subject.emtreeControlled studyen_US
dc.subject.emtreeDNA polymorphismen_US
dc.subject.emtreeFertilityen_US
dc.subject.emtreeFGF2 geneen_US
dc.subject.emtreeGeneen_US
dc.subject.scopusGrowth Hormone; Gene; Somatotropin Receptorsen_US
dc.subject.wosVeterinary sciencesen_US
dc.titleAssociations between GH, PRL, STAT5A, OPN, PIT-1, LEP and FGF2 polymorphisms and fertility in holstein-friesian heifersen_US
dc.title.alternativeHolstein-Friesian düvelerde fertilite ile GH, PRL, STAT5A, OPN, PIT-1, LEP ve FGF2 polimorfizimlerinin ilişkileritr_TR
dc.typeArticle
dc.wos.quartileQ2en_US

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