Characterization of staphylococcus aureus from humans and a comparison with isolates of animal origin, in North Dakota, United States

Simple item page
dc.contributor.authorVelasco, Valeria
dc.contributor.authorSherwood, Julie S.
dc.contributor.authorStepan, Ryan M.
dc.contributor.authorKoslofsky, Ryan J.
dc.contributor.authorLogue, Catherine M.
dc.contributor.buuauthorBüyükcangaz, Esra
dc.contributor.departmentUludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.tr_TR
dc.contributor.orcid0000-0003-4337-577Xtr_TR
dc.contributor.researcheridAAL-2323-2020tr_TR
dc.contributor.scopusid25649139400tr_TR
dc.date.accessioned2022-06-10T12:12:49Z
dc.date.available2022-06-10T12:12:49Z
dc.date.issued2015-10-20
dc.description.abstractDifferent clones of methicillin-susceptible (MSSA) and methicillin-resistant (MRSA) Staphylococcus aureus have been found in humans as well as in animals and retail meat. However, more information about the genetic characteristics and similarities between strains is needed. The aim of this study was to identify and characterize Staphylococcus aureus from humans, and to compare their characteristics with isolates of animal origin. A total of 550 nasal swabs were taken from healthy humans, and S. aureus was isolated and identified. Positive S. aureus isolates were subjected to molecular typing and susceptibility testing. In addition, 108 MRSA isolates recovered from clinical patients in the state of North Dakota and 133 S. aureus isolates from animals and meat previously analyzed were included. The nasal carriage of S. aureus in healthy people was 7.6% and, in general, clones were genetically diverse. None of the S. aureus strains obtained from healthy people were mecA-or PVL-positive. A total of 105 (97.2%) MRSA isolates from clinical cases harbored the mecA gene and 11 (10.2%) isolated from blood stream infections harbored the PVL gene. The most common resistance profile among S. aureus from healthy people was penicillin, and from clinical cases were erythromycin-penicillin-ciprofloxacin. The rate of multidrug resistance (MDR) was 70% in humans. Most of the S. aureus harboring mecA and PVL genes were identified as ST5 and ST8, and exhibited MDR. However, S. aureus isolates of animal origin used for comparison exhibited a lower rate of MDR. The most common resistance profiles in isolates of animal origin were penicillin-tetracycline and penicillin-tetracycline-erythromycin, in animals and raw meat, respectively. The ST5 was also found in animals and meat, with ST9 and ST398 being the major clones. The genetic similarity between clones from humans and meat suggests the risk of spread of S. aureus in the food chain.en_US
dc.description.sponsorshipDean's Office, College of Veterinary Medicine, Iowa State University, Ames, Iowaen_US
dc.description.sponsorshipDean's Office, College of Agriculture, Food Systems and Natural Resources College, North Dakota State Universityen_US
dc.identifier.citationVelasco, V. vd. (2015). "Characterization of staphylococcus aureus from humans and a comparison with isolates of animal origin, in North Dakota, United States". Plos One, 10(10).en_US
dc.identifier.issn1932-6203
dc.identifier.issue10tr_TR
dc.identifier.pubmed26484768tr_TR
dc.identifier.scopus2-s2.0-84948977770tr_TR
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0140497
dc.identifier.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0140497
dc.identifier.urihttp://hdl.handle.net/11452/27041
dc.identifier.volume10tr_TR
dc.identifier.wos000363028100038tr_TR
dc.indexed.pubmedPubMeden_US
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherPublic Library Scienceen_US
dc.relation.bapYDP (V)-2009/4tr_TR
dc.relation.collaborationYurt dışıtr_TR
dc.relation.journalPlos Onetr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMethicillin-resistanten_US
dc.subjectMrsaen_US
dc.subjectPrevalenceen_US
dc.subjectIdentificationen_US
dc.subjectInfectionsen_US
dc.subjectStrainsen_US
dc.subjectScience & technology - other topicsen_US
dc.subject.emtreeCiprofloxacinen_US
dc.subject.emtreeErythromycinen_US
dc.subject.emtreePenicillin derivativeen_US
dc.subject.emtreeTetracyclineen_US
dc.subject.emtreeBacterial proteinen_US
dc.subject.emtreeRNA 16Sen_US
dc.subject.emtreeAntibiotic resistanceen_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeBacterial geneen_US
dc.subject.emtreeBacterial strainen_US
dc.subject.emtreeBacterial transmissionen_US
dc.subject.emtreeBacterium identificationen_US
dc.subject.emtreeBacterium isolateen_US
dc.subject.emtreeCloneen_US
dc.subject.emtreeControlled studyen_US
dc.subject.emtreeDrug effecten_US
dc.subject.emtreeGenetic similarityen_US
dc.subject.emtreeGenetic variabilityen_US
dc.subject.emtreeHumanen_US
dc.subject.emtreeMecA geneen_US
dc.subject.emtreeMethicillin resistant Staphylococcus aureusen_US
dc.subject.emtreeMolecular typingen_US
dc.subject.emtreeNonhumanen_US
dc.subject.emtreeNormal humanen_US
dc.subject.emtreePVL geneen_US
dc.subject.emtreeStrain differenceen_US
dc.subject.emtreeUnited Statesen_US
dc.subject.emtreeAnimalen_US
dc.subject.emtreeBovineen_US
dc.subject.emtreeChickenen_US
dc.subject.emtreeClassificationen_US
dc.subject.emtreeCluster analysisen_US
dc.subject.emtreeComparative studyen_US
dc.subject.emtreeFood controlen_US
dc.subject.emtreeGeneticsen_US
dc.subject.emtreeIsolation and purificationen_US
dc.subject.emtreeMeaten_US
dc.subject.emtreeMethicillin resistant Staphylococcus aureusen_US
dc.subject.emtreeMicrobial sensitivity testen_US
dc.subject.emtreeMultidrug resistanceen_US
dc.subject.emtreeMicrobiologyen_US
dc.subject.emtreeMultilocus sequence typingen_US
dc.subject.emtreeNorth Dakotaen_US
dc.subject.emtreeNose cavityen_US
dc.subject.emtreePhylogenyen_US
dc.subject.emtreeProceduresen_US
dc.subject.emtreeSheepen_US
dc.subject.emtreeStaphylococcus aureusen_US
dc.subject.emtreeStaphylococcus infectionen_US
dc.subject.emtreeYoung adulten_US
dc.subject.meshAnimalsen_US
dc.subject.meshBacterial proteinsen_US
dc.subject.meshCattleen_US
dc.subject.meshChickensen_US
dc.subject.meshCluster analysisen_US
dc.subject.meshDrug resistance, multiple, bacterialen_US
dc.subject.meshFood microbiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshMeaten_US
dc.subject.meshMethicillin-resistant staphylococcus aureusen_US
dc.subject.meshMicrobial sensitivity testsen_US
dc.subject.meshMultilocus sequence typingen_US
dc.subject.meshNasal cavityen_US
dc.subject.meshNorth dakotaen_US
dc.subject.meshPhylogenyen_US
dc.subject.meshRNA, ribosomal, 16Sen_US
dc.subject.meshSheepen_US
dc.subject.meshStaphylococcal infectionsen_US
dc.subject.meshStaphylococcus aureusen_US
dc.subject.meshYoung adulten_US
dc.subject.scopusAnimals; Meticillin; Cefoxitinen_US
dc.subject.wosMultidisciplinary sciencesen_US
dc.titleCharacterization of staphylococcus aureus from humans and a comparison with isolates of animal origin, in North Dakota, United Statesen_US
dc.typeArticle
dc.wos.quartileQ1en_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Büyükcangaz_vd_2015.pdf
Size:
682.55 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Web of Science
Scopus