FHIT gene sequence variants and reduced fhit protein expression in glioblastoma multiforme

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dc.contributor.authorGüler, Gülnür
dc.contributor.authorAksoy, Kaya
dc.contributor.buuauthorÇeçener, Gülşah
dc.contributor.buuauthorTunca, Berrin Türkei
dc.contributor.buuauthorEgeli, Ünal
dc.contributor.buuauthorBekar, Ahmet
dc.contributor.buuauthorTolunay, Şahsine
dc.contributor.departmentUludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyoloji Anabilim Dalı.tr_TR
dc.contributor.departmentUludağ Üniversitesi/Tıp Fakültesi/Beyin ve Sinir Cerrahisi Anabilim Dalı.tr_TR
dc.contributor.departmentUludağ Üniversitesi/Tıp Fakültesi/Tıbbi Patoloji Anabilim Dalı.tr_TR
dc.contributor.orcid0000-0002-3820-424Xtr_TR
dc.contributor.orcid0000-0002-1619-6680tr_TR
dc.contributor.orcid0000-0001-7904-883Xtr_TR
dc.contributor.researcheridAAP-9988-2020tr_TR
dc.contributor.researcheridAAI-1612-2021tr_TR
dc.contributor.researcheridABI-6078-2020tr_TR
dc.contributor.researcheridAAH-1420-2021tr_TR
dc.contributor.scopusid6508156530tr_TR
dc.contributor.scopusid6602965754tr_TR
dc.contributor.scopusid55665145000tr_TR
dc.contributor.scopusid6603677218tr_TR
dc.contributor.scopusid6602604390tr_TR
dc.date.accessioned2022-09-09T12:56:18Z
dc.date.available2022-09-09T12:56:18Z
dc.date.issued2010-03
dc.description.abstractMolecular studies have an important role in the elucidation of the mechanisms involved in Glioblastoma multiforme (GBM) development. The occurrence of FHIT gene alterations, which has an important role in different cancers, has not yet been studied well in GBM. We aimed to investigate the occurrence of alterations of FHIT gene sequence and protein expression in the GBMs. Sequence alterations in exons 5-9 of the FHIT gene were screened in 63 GBMs using the single-strand conformational polymorphism method, followed by DNA sequencing. Additionally, the level of Fhit protein expression in tissues of 48 tumors was assessed by immunohistochemistry (IHC). In our investigation, FHIT gene alterations in the coding region were detected in 11 of the 63 GBM cases (17.5%). Two different sequence variants were determined: one novel missense variant (G -> C transition at codon 49) and one previously described silent alteration (C -> T transition at codon 88). Using web-based programs, such as SIFT and ESEfinder, it was determined that both alterations might have caused significant modification on protein function. In addition, we identified a previously reported an intronic polymorphism (T -> A transition at IVS8-17) in 47.5% of cases as a similar rate (45%) in the control group. Moreover, it was observed that Fhit protein expression was reduced in 87.5% of tumors. In conclusion, the reduction or loss of Fhit protein expression by genetic alterations or epigenetic mechanisms in GBM might be associated with brain tumorigenesis.en_US
dc.identifier.citationÇeçener, G. vd. (2010). "FHIT gene sequence variants and reduced fhit protein expression in glioblastoma multiforme". Cellular and Molecular Neurobiology, 30(2), 301-307.en_US
dc.identifier.endpage307tr_TR
dc.identifier.issn0272-4340
dc.identifier.issn1573-6830
dc.identifier.issue2tr_TR
dc.identifier.pubmed19760177tr_TR
dc.identifier.scopus2-s2.0-77952095741tr_TR
dc.identifier.startpage301tr_TR
dc.identifier.urihttps://doi.org/10.1007/s10571-009-9452-9
dc.identifier.urihttps://link.springer.com/article/10.1007/s10571-009-9452-9
dc.identifier.urihttp://hdl.handle.net/11452/28616
dc.identifier.volume30tr_TR
dc.identifier.wos000275704800014tr_TR
dc.indexed.pubmedPubMeden_US
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherSpringer/Plenum Publishersen_US
dc.relation.collaborationYurt içitr_TR
dc.relation.collaborationSanayitr_TR
dc.relation.journalCellular and Molecular Neurobiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGlioblastoma multiformeen_US
dc.subjectFHIT geneen_US
dc.subjectSequence alterationsen_US
dc.subjectSSCPen_US
dc.subjectIHCen_US
dc.subjectCommon fragile sitesen_US
dc.subjectCell lung-canceren_US
dc.subjectTumor-suppressor geneen_US
dc.subjectBrain-tumorsen_US
dc.subjectBreast-canceren_US
dc.subjectTurkish patientsen_US
dc.subjectPredispositionen_US
dc.subjectMutationsen_US
dc.subject3P14.2en_US
dc.subjectCarcinomaen_US
dc.subjectCell biologyen_US
dc.subjectNeurosciences & neurologyen_US
dc.subject.emtreeFragile histidine triad proteinen_US
dc.subject.emtreeAcid anhydride hydrolaseen_US
dc.subject.emtreeTumor proteinen_US
dc.subject.emtreeAdulten_US
dc.subject.emtreeAgeden_US
dc.subject.emtreeAmino acid substitutionen_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeCarcinogenesisen_US
dc.subject.emtreeControlled studyen_US
dc.subject.emtreeDNA sequenceen_US
dc.subject.emtreeFemaleen_US
dc.subject.emtreeGene mutationen_US
dc.subject.emtreeGene sequenceen_US
dc.subject.emtreeGenetic associationen_US
dc.subject.emtreeGenetic screeningen_US
dc.subject.emtreeGenetic variabilityen_US
dc.subject.emtreeGlioblastomaen_US
dc.subject.emtreeHumanen_US
dc.subject.emtreeHuman tissueen_US
dc.subject.emtreeImmunohistochemistryen_US
dc.subject.emtreeMajor clinical studyen_US
dc.subject.emtreeMaleen_US
dc.subject.emtreePriority journalen_US
dc.subject.emtreeProtein expressionen_US
dc.subject.emtreeProtein functionen_US
dc.subject.emtreeSingle strand conformation polymorphismen_US
dc.subject.emtreeBrain tumoren_US
dc.subject.emtreeGenetic variabilityen_US
dc.subject.emtreeGeneticsen_US
dc.subject.emtreeGlioblastomaen_US
dc.subject.emtreeMetabolismen_US
dc.subject.emtreeMiddle ageden_US
dc.subject.emtreeMolecular geneticsen_US
dc.subject.emtreeNucleotide sequenceen_US
dc.subject.meshAcid anhydride hydrolasesen_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshBase sequenceen_US
dc.subject.meshBrain neoplasmsen_US
dc.subject.meshFemaleen_US
dc.subject.meshGenetic variationen_US
dc.subject.meshGlioblastomaen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle ageden_US
dc.subject.meshMolecular sequence dataen_US
dc.subject.meshNeoplasm proteinsen_US
dc.subject.meshPolymorphism, single-stranded conformationalen_US
dc.subject.meshSequence analysis, DNAen_US
dc.subject.scopusFragile Histidine Triad Protein; Histidine; Triaden_US
dc.subject.wosCell biologyen_US
dc.subject.wosNeurosciencesen_US
dc.titleFHIT gene sequence variants and reduced fhit protein expression in glioblastoma multiformeen_US
dc.typeArticle
dc.wos.quartileQ3en_US

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