Comparing in vitro embryonic development of bovine oocytes cultured in G1.3/G2.3 sequential culture media and CRlaa medium

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dc.contributor.authorÇevik, Mesut
dc.contributor.authorTaş, Arzu
dc.contributor.authorAkkoç, Tolga
dc.contributor.authorBağış, Haydar
dc.contributor.authorArat, Sezen
dc.contributor.buuauthorSağırkaya, Hakan
dc.contributor.departmentUludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı.tr_TR
dc.contributor.researcheridAAH-8821-2021tr_TR
dc.contributor.scopusid6602400461tr_TR
dc.date.accessioned2022-03-22T11:26:14Z
dc.date.available2022-03-22T11:26:14Z
dc.date.issued2009
dc.description.abstractIn vitro embryo culture is an important step of in vitro production of bovine embryos. The aim of this study was to compare the in vitro development of bovine embryos in G1.3/G2.3 sequential culture media and CR1aa culture medium. Oocytes were obtained from ovaries of slaughtered cows and matured in TCM-199 medium in humidified air containing 5% CO(2) at 39 degrees C. Matured oocytes were fertilized in vitro using frozen bull sperm prepared with Percoll separation. After fertilization, the presumptive zygotes were denuded from cumulus cells and randomly allotted for 2 treatments: Culture in CR1aa (n = 116) and G1.3/G2.3 (n = 125). The embryo culture was carried out in 50 mu L droplets of the media that were placed in incubator containing 6% CO(2) 5% O(2) and 89% N(2). The embryos were evaluated on days 3, 5 and 7 following in vitro fertilization. The difference of cleavage rates between 2 treatments was not found significant (p>0.05). However, significant difference was observed between 2 treatments at the level of the obtaining rate of morula and blastocyst (p<0.05). The results showed that G1.3/G2.3 sequential media significantly favoured developing of morula and blastocysts in comparison to CR1aa medium (32.0% versus 10.40% versus 18.96 and 2.58%, respectively).en_US
dc.description.sponsorshipOndokuz Mayıs Üniversitesi (VET-011)tr_TR
dc.identifier.citationÇevik, M. vd. (2009). "Comparing in vitro embryonic development of bovine oocytes cultured in G1.3/G2.3 sequential culture media and CRlaa medium". Journal of Animal and Veterinary Advances, 8(6), 1185-1189.en_US
dc.identifier.endpage1189tr_TR
dc.identifier.issn1680-5593
dc.identifier.issue6tr_TR
dc.identifier.scopus2-s2.0-70350767516tr_TR
dc.identifier.startpage1185tr_TR
dc.identifier.urihttp://hdl.handle.net/11452/25266
dc.identifier.volume8tr_TR
dc.identifier.wos000265889100028tr_TR
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherMedwell Onlineen_US
dc.relation.collaborationYurt içitr_TR
dc.relation.collaborationSanayitr_TR
dc.relation.journalJournal of Animal and Veterinary Advancesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBovineen_US
dc.subjectCRlaaen_US
dc.subjectEmbryo cultureen_US
dc.subjectGl.3/G2.3 sequential mediaen_US
dc.subjectIVFen_US
dc.subjectAmino-acidsen_US
dc.subjectFertilizationen_US
dc.subjectBlastocystsen_US
dc.subjectMaturationen_US
dc.subjectSerumen_US
dc.subjectCompetenceen_US
dc.subjectCleavageen_US
dc.subjectVitaminsen_US
dc.subjectQualityen_US
dc.subjectVeterinary sciencesen_US
dc.subject.scopusBovine Embryo; Blastocyst; In Vitro Studiesen_US
dc.subject.wosVeterinary sciencesen_US
dc.titleComparing in vitro embryonic development of bovine oocytes cultured in G1.3/G2.3 sequential culture media and CRlaa mediumen_US
dc.typeArticle
dc.wos.quartileQ4en_US

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