MicroRNA expression patterns of CD8+ T cells in acute and chronic brucellosis

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dc.contributor.authorGüvenç, Furkan
dc.contributor.authorDeniz, Günnur
dc.contributor.buuauthorBudak, Ferah
dc.contributor.buuauthorBal, S. Haldun
dc.contributor.buuauthorTezcan, Gülçin
dc.contributor.buuauthorAkalın, E. Halis
dc.contributor.buuauthorGöral, Güher
dc.contributor.buuauthorOral, H. Barbaros
dc.contributor.departmentUludağ Üniversitesi/Tıp Fakültesi/İmmünoloji Anabilim Dalı.tr_TR
dc.contributor.departmentUludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyoloji Anabilim Dalı.tr_TR
dc.contributor.departmentUludağ Üniversitesi/Tıp Fakültesi/Klinik Mikrobiyoloji ve Enfeksiyon Hastalıkları Anabilim Dalı.tr_TR
dc.contributor.departmentUludağ Üniversitesi/Tıp Fakültesi/Tıbbi Mikrobiyoloji Anabilim Dalı.tr_TR
dc.contributor.orcid0000-0003-0463-6818tr_TR
dc.contributor.orcid0000-0002-5956-8755tr_TR
dc.contributor.researcheridAAU-8952-2020tr_TR
dc.contributor.researcheridK-7285-2012tr_TR
dc.contributor.researcheridAAH-3843-2020tr_TR
dc.contributor.researcheridF-8554-2017tr_TR
dc.contributor.researcheridF-4657-2014tr_TR
dc.contributor.scopusid6701913697tr_TR
dc.contributor.scopusid57191480128tr_TR
dc.contributor.scopusid25650627600tr_TR
dc.contributor.scopusid57207553671tr_TR
dc.contributor.scopusid6603453166tr_TR
dc.contributor.scopusid7004498001tr_TR
dc.date.accessioned2022-11-29T11:13:37Z
dc.date.available2022-11-29T11:13:37Z
dc.date.issued2016-10-06
dc.description.abstractAlthough our knowledge about Brucella virulence factors and the host response increase rapidly, the mechanisms of immune evasion by the pathogen and causes of chronic disease are still unknown. Here, we aimed to investigate the immunological factors which belong to CD8+ T cells and their roles in the transition of brucellosis from acute to chronic infection. Using miRNA microarray, more than 2000 miRNAs were screened in CD8+ T cells of patients with acute or chronic brucellosis and healthy controls that were sorted from peripheral blood with flow cytometry and validated through qRT-PCR. Findings were evaluated using GeneSpring GX (Agilent) 13.0 software and KEGG pathway analysis. Expression of two miRNAs were determined to display a significant fold change in chronic group when compared with acute or control groups. Both miRNAs (miR-126-5p and miR-47533p) were decreased (p <0.05 or fold change > 2). These miRNAs have the potential to be the regulators of CD8+ T cell-related marker genes for chronic brucellosis infections. The differentially expressed miRNAs and their predicted target genes are involved in MAPK signaling pathway, cytokine-cytokine receptor interactions, endocytosis, regulation of actin cytoskeleton, and focal adhesion indicating their potential roles in chronic brucellosis and its progression. It is the first study of miRNA expression analysis of human CD8+ T cells to clarify the mechanism of inveteracy in brucellosis.en_US
dc.identifier.citationBudak, F. vd. (2016). "MicroRNA expression patterns of CD8+ T cells in acute and chronic brucellosis". Plos One, 11(11).en_US
dc.identifier.issn1932-6203
dc.identifier.issue11tr_TR
dc.identifier.pubmed27824867tr_TR
dc.identifier.scopus2-s2.0-84994735100tr_TR
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0165138
dc.identifier.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0165138
dc.identifier.urihttp://hdl.handle.net/11452/29625
dc.identifier.volume11tr_TR
dc.identifier.wos000387615200014tr_TR
dc.indexed.pubmedPubMeden_US
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherPublic Library Scienceen_US
dc.relation.bapUAP(T)-2011/15en_US
dc.relation.collaborationYurt içitr_TR
dc.relation.collaborationYurt dışıtr_TR
dc.relation.journalPlos Oneen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectScience & technology - other topicsen_US
dc.subjectT-lymphocytesen_US
dc.subjectUp-regulationen_US
dc.subjectDifferential expressionen_US
dc.subjectProstate-canceren_US
dc.subjectDown-regulationen_US
dc.subjectPoor-prognosisen_US
dc.subjectSerum mirnasen_US
dc.subjectHepatitis-Ben_US
dc.subjectProliferationen_US
dc.subjectMelitensisen_US
dc.subject.emtreeJanus kinaseen_US
dc.subject.emtreeMicroRNAen_US
dc.subject.emtreeMicroRNA 126 5pen_US
dc.subject.emtreeMicroRNA 4753 3pen_US
dc.subject.emtreeMitogen activated protein kinaseen_US
dc.subject.emtreeSTAT proteinen_US
dc.subject.emtreeT lymphocyte receptoren_US
dc.subject.emtreeUnclassified drugen_US
dc.subject.emtreeActin filamenten_US
dc.subject.emtreeAcute diseaseen_US
dc.subject.emtreeAdulten_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeBlooden_US
dc.subject.emtreeBrucellosisen_US
dc.subject.emtreeCD8+ T lymphocyteen_US
dc.subject.emtreeChronic diseaseen_US
dc.subject.emtreeClinical articleen_US
dc.subject.emtreeControlled studyen_US
dc.subject.emtreeDisease courseen_US
dc.subject.emtreeEndocytosisen_US
dc.subject.emtreeFemaleen_US
dc.subject.emtreeFlow cytometryen_US
dc.subject.emtreeFocal adhesionen_US
dc.subject.emtreeGene expressionen_US
dc.subject.emtreeGene functionen_US
dc.subject.emtreeGene targetingen_US
dc.subject.emtreeHeredityen_US
dc.subject.emtreeHumanen_US
dc.subject.emtreeHuman cellen_US
dc.subject.emtreeHuman tissueen_US
dc.subject.emtreeImmune responseen_US
dc.subject.emtreeMaleen_US
dc.subject.emtreeMarker geneen_US
dc.subject.emtreeMicroarray analysisen_US
dc.subject.emtreeMiddle ageden_US
dc.subject.emtreeProtein functionen_US
dc.subject.emtreeProtein protein interactionen_US
dc.subject.emtreeQuantitative analysisen_US
dc.subject.emtreeReal time polymerase chain reactionen_US
dc.subject.emtreeSignal transductionen_US
dc.subject.emtreeSoftwareen_US
dc.subject.emtreeWnt signaling pathwayen_US
dc.subject.emtreeAcute diseaseen_US
dc.subject.emtreeBrucellosisen_US
dc.subject.emtreeCD8+ T lymphocyteen_US
dc.subject.emtreeChronic diseaseen_US
dc.subject.emtreeGene expression profilingen_US
dc.subject.emtreeHost pathogen interactionen_US
dc.subject.emtreeImmune evasionen_US
dc.subject.emtreeMetabolismen_US
dc.subject.emtreePhysiologyen_US
dc.subject.emtreeProceduresen_US
dc.subject.meshAcute diseaseen_US
dc.subject.meshAdulten_US
dc.subject.meshBrucellosisen_US
dc.subject.meshCD8-positive T-lymphocytesen_US
dc.subject.meshChronic diseaseen_US
dc.subject.meshDisease progressionen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene expression profilingen_US
dc.subject.meshHost-pathogen interactionsen_US
dc.subject.meshHumansen_US
dc.subject.meshImmune evasionen_US
dc.subject.meshMaleen_US
dc.subject.meshMicroRNAsen_US
dc.subject.meshMiddle ageden_US
dc.subject.meshSignal transductionen_US
dc.subject.scopusAnimals; Zoonosis; Bovine Brucellosisen_US
dc.subject.wosMultidisciplinary sciencesen_US
dc.titleMicroRNA expression patterns of CD8+ T cells in acute and chronic brucellosisen_US
dc.typeArticle
dc.wos.quartileQ1en_US

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