Analysis of IGf1 C472t and TG C422t polymorphisms in Turkish grey steppe and holstein crossbred bulls

Simple item page
dc.contributor.buuauthorArdıçlı, Sena
dc.date.accessioned2020-12-21T08:00:13Z
dc.date.available2020-12-21T08:00:13Z
dc.date.issued2018-03-01
dc.description.abstractThe aim of the study was to determine genotypic distribution of bovine insulin-like growth factor 1 and thyroglobulin genes in Turkish Grey Steppe (Boz) and Holstein crossbred bulls. In the study, unrelated 30 Turkish Grey and 26 Holstein crossbred cattle were used. Genomic DNA was isolated from whole blood using standart phenol-chloroform extraction method. The polymerase chain reaction-restriction fragment length polymorphism technique was used for genotyping of C472T and C422T polymorphisms in insulin-like growth factor 1 and thyroglobulin genes, respectively. Hardy-Weinberg equilibrium was calculated by using PopGene software program. In addition the population indexes including heterozygosity, number of effective alleles and polymorphism information content were determined on the basis of allelic distribution. For insulin-like growth factor 1 gene, A allele frequency was higher and BB genotype was not present in both breed groups. For thyroglobulin gene, TT genotype was not present and moreover C allele was almost fixed in both Turkish Grey and Holstein crossbred bulls. In addition, extremely low minor allele frequencies in resulted in low genetic variabilities of population indexes. The present results may be useful for future studies conducted on bovine genome with respect to insulin-like growth factor 1 and thyroglobulin genes.en_US
dc.identifier.citationArdıçlı, S. (2018). "Analysis of IGf1 C472t and TG C422t polymorphisms in Turkish grey steppe and holstein crossbred bulls". Uludağ Üniversitesi Veteriner Fakültesi, 37(1), 21-28.tr_TR
dc.identifier.endpage28tr_TR
dc.identifier.issn1301-3173
dc.identifier.issue1tr_TR
dc.identifier.startpage21tr_TR
dc.identifier.urihttps://dergipark.org.tr/tr/download/article-file/477454
dc.identifier.urihttp://hdl.handle.net/11452/14489
dc.identifier.volume37tr_TR
dc.language.isoenen_US
dc.publisherUludağ Üniversitesitr_TR
dc.relation.journalVeteriner Hekimlikte Araştırma Dergisi / Journal of Research in Veterinary Medicinetr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCattleen_US
dc.subjectTurkish greyen_US
dc.subjectHolstein crossbredsen_US
dc.subjectIGF1en_US
dc.subjectTGen_US
dc.subjectPCR-RFLPen_US
dc.titleAnalysis of IGf1 C472t and TG C422t polymorphisms in Turkish grey steppe and holstein crossbred bullsen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
37_1_3.pdf
Size:
810.78 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

2018 Cilt 37 Sayı 1