Bioassay-guided isolation of cytotoxic compounds from Chrysophthalmum montanum (DC.) Boiss
dc.contributor.author | Ayaz, Fatma | |
dc.contributor.author | Küçükboyacı, Nurgün | |
dc.contributor.author | Gören, Nezhun Ates | |
dc.contributor.author | Çalış, İhsan | |
dc.contributor.author | Ulukaya, Engin | |
dc.contributor.author | Duman, Hayri̇ D. | |
dc.contributor.author | Choudhary, Mohammed Iqbal | |
dc.contributor.buuauthor | Aydınlık, Şeyma | |
dc.contributor.department | Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Biyoloji Bölümü. | tr_TR |
dc.contributor.orcid | 0000-0001-5238-2432 | tr_TR |
dc.contributor.researcherid | ABI-2909-2020 | tr_TR |
dc.contributor.scopusid | 57190280044 | tr_TR |
dc.date.accessioned | 2024-01-09T06:54:17Z | |
dc.date.available | 2024-01-09T06:54:17Z | |
dc.date.issued | 2018-12-21 | |
dc.description.abstract | Bioassay-guided isolation of the 80% methanol extract of the aerial parts of Chrysophthalmum montanum (DC.) Boiss. (Asteraceae) led to the isolation of four known guaianolide-type sesquiterpene lactones, 6 alpha-acetoxy-4 alpha-hydroxy-113H-guaia-9.11(13)-dien-12.8a-olide (1), 6 alpha-acetoxy-4 alpha-hydroxy-9 beta.10 beta-epoxy-1 beta H-guaia-11(13)-en12.8 alpha-olide (2), 4 alpha,6 alpha-dihydroxy-1 beta H,5 alpha,7 alpha H-guaia-9(10),11(13)-dien-12,8 alpha-olide (3), and (4 alpha,5 alpha,8 beta,10 beta)-4,10-dihydroxy-1,11(13)-guaidien-12,8-olide (4), along a steroidal glycoside mixture (5a and 5b). The structures of the compounds were identified on the basis of spectroscopic data. Among them, 2, 4 and a steroidal glycoside mixture were obtained from C. montanum for the first time. All isolates were also first time assayed for in vitro cytotoxicities against four human cancer cell lines, i.e. breast (MCF-7, MDA-MB 231), colon (FIT-29), and lung (PC3). Among the isolates, 1-3 showed significant inhibitory effect on the proliferation of cancer cells with viability ranging from 6.86 to 26.51%, while steroidal glycoside mixture showed no cytotoxicity, except against HT-29 (viability 61.99%). Compound 4 exhibited strong and selective cell growth inhibition against HT-29 with viability 20.99% and was identified as a promising compound with high selectivity between cancer cells and normal human lung cells (SEAS-2B), especially against HT-29 (IC50 = 12.2 pg/mL) compared to that of cisplatin. These results suggested that 4 is worthy of further study to determine its cytotoxicity mechanisms. | en_US |
dc.identifier.citation | Aydınlık, Ş. vd. (2018). "Bioassay-guided isolation of cytotoxic compounds from Chrysophthalmum montanum (DC.) Boiss". Journal of the Nepal Medical Association, 125, 10-20. | en_US |
dc.identifier.endpage | 20 | en_US |
dc.identifier.issn | 0278-6915 | |
dc.identifier.issn | 1873-6351 | |
dc.identifier.pubmed | 30580030 | tr_TR |
dc.identifier.scopus | 2-s2.0-85059058009 | tr_TR |
dc.identifier.startpage | 10 | tr_TR |
dc.identifier.uri | https://doi.org/10.1016/j.fct.2018.12.029 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0278691518309025?via%3Dihub | |
dc.identifier.uri | https://hdl.handle.net/11452/38871 | |
dc.identifier.volume | 125 | tr_TR |
dc.identifier.wos | 000463305000002 | tr_TR |
dc.indexed.pubmed | PubMed | en_US |
dc.indexed.wos | SCIE | |
dc.language.iso | en | tr_TR |
dc.publisher | Elsevier | en_US |
dc.relation.collaboration | Yurt içi | tr_TR |
dc.relation.collaboration | Yurt dışı | tr_TR |
dc.relation.journal | Food and Chemical Toxicology | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | tr_TR |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Asteraceae | en_US |
dc.subject | Steroidal glycosides | en_US |
dc.subject | Chrysophthalmum montanum | en_US |
dc.subject | Sesquiterpene lactones | en_US |
dc.subject | Cytotoxicity | en_US |
dc.subject | HT-29 colon cancer cell line | en_US |
dc.subject | Sesquiterpene lactones | en_US |
dc.subject | Plants | en_US |
dc.subject | Constituents | en_US |
dc.subject | Inuleae | en_US |
dc.subject | Asteraceae | en_US |
dc.subject | Extracts | en_US |
dc.subject | Guaianolides | en_US |
dc.subject | Food science & technology | en_US |
dc.subject | Toxicology | en_US |
dc.subject.emtree | Antineoplastic activity | en_US |
dc.subject.emtree | Article | en_US |
dc.subject.emtree | Asteraceae | en_US |
dc.subject.emtree | Beas-2b cell line | en_US |
dc.subject.emtree | Bioassay | en_US |
dc.subject.emtree | Cancer inhibition | en_US |
dc.subject.emtree | Cell proliferation | en_US |
dc.subject.emtree | Cell viability | en_US |
dc.subject.emtree | Chrysophthalmum montanum | en_US |
dc.subject.emtree | Controlled study | en_US |
dc.subject.emtree | Drug cytotoxicity | en_US |
dc.subject.emtree | Drug mechanism | en_US |
dc.subject.emtree | Drug structure | en_US |
dc.subject.emtree | Ht-29 cell line | en_US |
dc.subject.emtree | Ic50 | en_US |
dc.subject.emtree | In vitro study | en_US |
dc.subject.emtree | Lung alveolus cell | en_US |
dc.subject.emtree | Mcf-7 cell line | en_US |
dc.subject.emtree | Mda-mb-231 cell line | en_US |
dc.subject.emtree | Nonhuman | en_US |
dc.subject.emtree | Spectroscopy | en_US |
dc.subject.emtree | Structure analysis | en_US |
dc.subject.emtree | Aerial plant part | en_US |
dc.subject.emtree | Chemistry | en_US |
dc.subject.emtree | Drug screening | en_US |
dc.subject.emtree | Human | en_US |
dc.subject.emtree | Isolation and purification | en_US |
dc.subject.emtree | Procedures | en_US |
dc.subject.emtree | Tumor cell line | en_US |
dc.subject.emtree | (4 alpha 5 alpha 8 beta 10 beta) 4,10 dihydroxy 1,11(13) guaidien 12,8 olide | en_US |
dc.subject.emtree | (4 alpha 5 alpha 8 beta) 4,10 dihydroxy 1,11(13) guaidien 12,8 olide | en_US |
dc.subject.emtree | 4 alpha, 6 alpha dihydroxy 1 beta, 5 alpha, 7 alpha h guaia 9(10),11(13) dien 12,8 alpha olide | en_US |
dc.subject.emtree | 6 alpha acetoxy 4 alpha hydroxy 1 beta h guaia 9,11(13) dien 12,8 alpha olide | en_US |
dc.subject.emtree | 6 alpha acetoxy 4 alpha hydroxy 9 beta, 10 beta h guaia 9,11(13) dien 12,8 alpha olide | en_US |
dc.subject.emtree | Antineoplastic agent | en_US |
dc.subject.emtree | Cisplatin | en_US |
dc.subject.emtree | Cytotoxic agent | en_US |
dc.subject.emtree | Sitogluside | en_US |
dc.subject.emtree | Sitosterol 3 o beta glucopyranoside | en_US |
dc.subject.emtree | Unclassified drug | en_US |
dc.subject.emtree | Antineoplastic agent | en_US |
dc.subject.mesh | Antineoplastic agents, phytogenic | en_US |
dc.subject.mesh | Asteraceae | en_US |
dc.subject.mesh | Biological assay | en_US |
dc.subject.mesh | Cell line, tumor | en_US |
dc.subject.mesh | Drug screening assays, antitumor | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Plant components, aerial | en_US |
dc.subject.mesh | Spectrum analysis | en_US |
dc.subject.scopus | Inula; Asteraceae; Dittrichia Viscosa | en_US |
dc.subject.wos | Food science & technology | en_US |
dc.subject.wos | Toxicology | en_US |
dc.title | Bioassay-guided isolation of cytotoxic compounds from Chrysophthalmum montanum (DC.) Boiss | en_US |
dc.type | Article | en_US |
dc.wos.quartile | Q1 |
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