Geçici hipotermi ve nimodipinin iskemik hacim üzerine etkisi
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Date
1992-09-03
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Uludağ Üniversitesi
Abstract
Bu çalışmada standalt iskemi modeli oluşturulan sıçanlarda nimodipin ve hipotenninin iskemi üzerine etkisi incelendi. Birinci grupta (n: 10) Ona Serebral Alter (OSA) oklüzyonun dan sonra nimodipin verilirken, ikinci grupta (n: 10) OSA oklüzyonu yapılmadan önce sıçanların rektal ısılan 22 C'ye düşürüldü ve daha sonra oklüzyon yapılarak hipotermi uygulandı. Operasyondan 24 saat sonra dekapite edilen sıçanların beyinleri hızla çıkanlarak koronal kesitler alındı ve 2, 3, 5-triphenyl/tetrazolium chloride (TTC) ile boyanarak tespit edildi. İskemik alanların bilgisayar görüntüleme metodu ile hacimleri hesaplandı. Gruplann infarkı hacimleri istatistiki olarak birbirleri ile kıyaslandığında nimodipin ve hipotermi grubunda kontrol grubuna göre anlamlı derecede (p < 0.01) bir azalma olduğu gözlendi. Sonuç olarak nimodipin ve hipoterminin iskemi sonrası oluşan infarkt volümü üzerine azaltıcı etkisi olduğu saptandı.
In this experiment the effect of hypothermia and nimodipine was evaluated using a rat model of standard brain ischemia. In the first group (n: 10) nimodipine was administered after middle cerebral artery (MCA) occlusion while in the second group (n: 10) MCA occlusion was performed at a rectal temperature 22°C and kept in this temperature for one hour. Animals in both groups were decapitated 24 hours after operation and cerebral infarcts were visualized by staining of coronal brain sections with 2, 3, 5-triphenyltetrazolium chloride (TTC). The volume of ischemic areas were calculated by a method of computerized imaging. The infarct volume was to be decreased in nimodipine and hypotlı mıic group when compared with the control group and it was statistically significant (p < 0.01). These results suggest that nimodipine and hypothermia reduces subsequent infarct volume in MCA occlusion.
In this experiment the effect of hypothermia and nimodipine was evaluated using a rat model of standard brain ischemia. In the first group (n: 10) nimodipine was administered after middle cerebral artery (MCA) occlusion while in the second group (n: 10) MCA occlusion was performed at a rectal temperature 22°C and kept in this temperature for one hour. Animals in both groups were decapitated 24 hours after operation and cerebral infarcts were visualized by staining of coronal brain sections with 2, 3, 5-triphenyltetrazolium chloride (TTC). The volume of ischemic areas were calculated by a method of computerized imaging. The infarct volume was to be decreased in nimodipine and hypotlı mıic group when compared with the control group and it was statistically significant (p < 0.01). These results suggest that nimodipine and hypothermia reduces subsequent infarct volume in MCA occlusion.
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Keywords
Kalsiyum kanal blokerleri, Hipotermi, İskemi, Calcium channel blockers, Hypothermia, Ischemia
Citation
Doygun, M. vd. (1993). ''Geçici hipotermi ve nimodipinin iskemik hacim üzerine etkisi''. Uludağ Üniversitesi Tıp Fakültesi Dergisi, 20(1), 39-42.