Sıçanlarda östrus döngüsü ile ilişkili ovaryum ve uterusların histolojik değerlendirmesi
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Date
2015-06-19
Authors
İnan, Sevinç
Öktem, Gülperi
Onur, Ece
Özbilgin, Kemal
Journal Title
Journal ISSN
Volume Title
Publisher
Uludağ Üniversitesi
Abstract
İnsanlarda 28 günde izlenen menstrüel döngünün, sıçanlarda 4-5 günde gerçekleşmesinden dolayı, kadın üreme sisteminin incelendiği çalışmalarda model organizma olarak kullanılan sıçanlar ile yapılan birçok çalışma kısa sürede tamamlanabilmektedir. Bu da sıçan modelinin bir avantajı olarak görülmektedir. Bu çalışmada sıçan östrus döngüsü ile ilgili sitolojik ve histolojik değerlendirmeler ve elde edilen son literatür bilgilerinin derlenmesi ile yeni çalışmalar için kaynak oluşturulması amaçlanmıştır. Vaginal yayma ile dişi sıçanlar proöstrus, östrus, metöstrus, diöstrus dönemlerinde olmak üzere 4 gruba ayrıldı ve grupları belirlenen dişi sıçanlardan (n=28) ovaryum ve uterus örnekleri elde edildi. Dokular formalinde tespit edilerek, rutin parafin doku takibi ile parafine gömüldü. Parafine gömülmüş dokulardan 5 µm.’lik kesitler alınarak Hematoksilen-Eozin (H-E) ve Periyodik Asit Shiff (PAS) ile boyandı. Boyanmış kesitlerde ovaryum ve uterustaki temel yapılar değerlendirildi ve istatistiksel olarak karşılaştırıldı. Kesitlerde görülen temel yapıların sayıları gruplar ile uyumlu olarak bulunmuş olup, proöstrus ve östrus gruplarında artmış primer ve sekonder folliküller izlenirken, metöstrus grubunda korpus luteum yapılarının istatistiksel anlamlı olarak arttığı gözlendi. Çalışmada sıçan östrus döngüsü evrelerinde elde edilen ovaryum ve uteruslara ait histokimyasal ve sayısal veriler geçmiş literatür ile uyumlu bulundu. Buradaki değerlendirmelerin bundan sonraki çalışmalar için kaynak niteliği taşıyacağı düşünülmektedi.
Studies examining the female reproductive system in the rat model can be completed in a short time due to the 4-5-day-long estrous cycle, which is an advantage when compared to 28 days of menstrual cycle in humans. The present study aimed to create new insights for cytological and histological studies evaluating the rat estrous cycle with the compilation of the recent literature. Female rats were divided into 4 groups according to the vaginal smear examinations, as proestrous, estrous, metestrous and diestrous groups and ovaries as well as uterus samples were obtained. The samples were fixed in 10% formaline solution and prepared according the routine paraffin tissue processing protocol and embedded in paraffin. Five-micrometer-thick paraffin-embedded sections were stained with H-E and PAS. By microscopical analyzing the stained sections, primary structures in the ovary and uterus were assessed and the results were statistically compared between groups. Numbers of primary structures observed in the sections were compatible with the respective groups. While increased numbers of primary and secondary follicles in the groups of proestrous and estrous were detected, in metestrous group increased number of corpus luteum was obtained. The data obtained from different stages of rat estrous cycle were in agreement with the reports in the literature. It is suggested that the results of this study can be used as a source to plan advanced histological studies.
Studies examining the female reproductive system in the rat model can be completed in a short time due to the 4-5-day-long estrous cycle, which is an advantage when compared to 28 days of menstrual cycle in humans. The present study aimed to create new insights for cytological and histological studies evaluating the rat estrous cycle with the compilation of the recent literature. Female rats were divided into 4 groups according to the vaginal smear examinations, as proestrous, estrous, metestrous and diestrous groups and ovaries as well as uterus samples were obtained. The samples were fixed in 10% formaline solution and prepared according the routine paraffin tissue processing protocol and embedded in paraffin. Five-micrometer-thick paraffin-embedded sections were stained with H-E and PAS. By microscopical analyzing the stained sections, primary structures in the ovary and uterus were assessed and the results were statistically compared between groups. Numbers of primary structures observed in the sections were compatible with the respective groups. While increased numbers of primary and secondary follicles in the groups of proestrous and estrous were detected, in metestrous group increased number of corpus luteum was obtained. The data obtained from different stages of rat estrous cycle were in agreement with the reports in the literature. It is suggested that the results of this study can be used as a source to plan advanced histological studies.
Description
II. Sağlık Bilimleri Enstitüleri Lisansüstü Öğrenci Kongresi ve Kök Hücre Sempozyumu’nda (Kuşadası 2014) sözlü bildiri olarak sunulmuştur.
Keywords
Ovulasyon, Sıçan östrus döngüsü, Ovaryum, Uterus, Rat estrous cycle, Ovulation, Uteri, Ovary
Citation
Ekizceli, G. vd. (2015). ''Sıçanlarda östrus döngüsü ile ilişkili ovaryum ve uterusların histolojik değerlendirmesi''. Uludağ Üniversitesi Tıp Fakültesi Dergisi, 41(2), 65-72.