Subklinik mastitislerin teşhis yöntemleri üzerine çalışmalar
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Date
1999-03-09
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Uludağ Üniversitesi
Abstract
Bu çalışma, California Mastitis Test (CMT). Direkt Mikroskobik Somatik Hücre Sayımı (DMSHS), ayırıcı hücre sayımı bulgularına göre normal ve subklinik mastitisli olarak ayrılan süf örneklerinde çeşitli biyokimyasal testleri karşılaştırmalı olarak incelemek ve bu testlerin ineklerde subklinik mastitislerin teşhisinde kullanılabilirliğini saptamak için yapıldı. Bu çalışma da, Uludağ Üniversitesi Veteriner Fakültesi Uygulama ve Araştırma Çiftliğinde ve Bursa bölgesindeki ticari sütçü işletmelerde bulunan 3-7 yaşlı, Holstein, Montofon ırklarına ait 100 adet inek araştırma materyali olarak kullanıldı. Bütün ineklere ilk önce CMI uygulandı. CMT sonuçlarına göre "-" reaksiyon gösteren 30 meme lobu sağlıklı, "2. 7+. 2+, 3+" reaksiyon gösteren 70 meme lobu subklinik mastitisli olarak ayrıldı. Daha sonra, DMSHS ve Ayırıcı hücre sayımları yapıldı. Sağlıklı ve subklinik mastitisli ineklerden toplanan süt serumlarının mineral madde analiz sonuçları sırasıyla; Na, % 43.37 = 2.92mg - % 58.51 3.68mg (P<0.005); K. % 97.53 + 4.44mg - % 80.37 + 4.56mg (P<0.02) CI, % 72.95 + 3.26mg - % 108,27 + 4.50mg (P<0.001); Pi. % 2.34 + 0.41 mg - % 2.29 + 0.04mg (P>0.90): Mg, % 2.63 + 0.07mg - % 2.57 0.04mg (P>0.20); Ca, % 40.36 2.29mg - % 48.05 + 1.85mg (P<0.05) olarak belirlendi. Sağlıklı ve subklinik mastitisli ineklerden toplanan sür serumlarının enzim analiz sonuçları sırasıyla; LDH, 39.83 € 10.98 U/L - 410.47 + 59.26 UL (P<0.001): AST, 2.5 + 0.71 U/L - 22.74 + 4.92 U/L (P<0.02): ALT, 4.5+ 0.99 U/L - 12.23 = 1.88 U/L (P<0.05), Amilaz, 5.46 € 1.53 U/L - 6.67 + 1.86 UL (P>0.50): GGT. 1326.38 + 100.37 U/L - 1710.77 + 151.53 U/L (P>0.10); CK 4.86 + 0.68 UL - 14.04 + 1.91 UL (P<0.005). ALP. 261.47 + 34.25 U/L - 723.79 + 64.66 U/L (P<0.001): ASP. 0.63 + 0.20 U/L - 2.16 + 0.34 U/L (P<0.005) olarak saptandı. Sağlıklı ve subklinik mastitisli ineklerden toplanan süt serumlarının metabolit analiz sonuçları sırasıyla; glikoz, 3.52 + 0.42 mg/dl - 1.53 0.15 mg/dl (P<0.001): kolesterol, 7.27 + 0.76 mg/dl - 8.04 + 0.57 mg/dl (P>0.20): trigliserid, 19.33 + 1.71 mg/dl - 25.64 1.81 mg/dl (P>0.05) olarak bulundu. Sonuç olarak, sütte yapılan direkt ve indirekt somatik hücre sayımlarının, süt serumlarında Na, K, CI gibi mineral maddelerin, LDH, AST, ALT. CK ALP. ASP enzim aktivitelerinin, glikoz metabolitinin saptanmasının subklinik mastitislerin teşhisinde başarılı bir şekilde kullanılabileceği sonucuna varıldı.
This study was conducted to investigate comparatively various biochemical test on normal and subclinical mastitic milk samples, diagnosed according to the California Mastitis Test (CMT), Direct Microscopic Somatic Cell Count (DMSCC). Differential Cell Count (DCC) results and to establish usefulness of these tests in the diagnosis of subclinical mastitis cases in cows. In the present, a total of 700 Holstein, Brown-Swiss cows, in 3-7 years old, supplied by Faculty of Veterinary Medicine University of Uludağ and commercial dairy farms in the region of research materials. CMT was performed previously on milk samples obtained from all cows. According to the test results. 30 mammary quarters shown" - " reactions were identified as free from subclinical mastitis. The other 70 mammary quarters reacted "?. I+, 2+. 3+" were identified as subclinical mastitis. Than, DMSCC and DCC were done. The results of mineral elements analysis of milk serum samples collected from healthy and subclinical mastitis cowe were shown as Na, % 43.37 = 2.92mg - % 58.51 3.68mg (P<0.005); K, % 97.53# 4.44mg - % 80.37 + 4.56mg (P<0.02): CI, % 72.95 + 3.26mg - % 108.27 = 4.50mg (P<0.001): Pi. % 2.34 + 0.41 mg - % 2.29 + 0.04mg (P>0.90). Mg. % 2.63 + 0.07mg - % 2.51 +0.04mg (P>0.20); Ca, % 40.36 = 2.29mg - % 48.05 + 1.85mg (P<0.05), respectively. The results of enzymes activity analysis of milk serum samples collected from healthy and subclinical mastitis cows were as belows; LDH, 39.83 = 10.98 U/L - 410.47 59.26 U/L (P<0.001), AST, 2.5 +0.77 U/L - 22.74 + 4.92 U/L (P<0.02), ALT: 4.5 +0.99 U/L. - 12.23 + 7.88 U/L (P<0.05); Amylase, 5.46 + 1.53 U/L - 6.67 + 1.86 U/L (P>0.50): GGT 7326.38 + 160.37 U/L - 1710.77 157.53 U/L (P>0.10): CK 4.86 + 0.68 U/L - 14.04 + 7.97 U/L (P<0.005): ALP. 261.47 + 34.25 U/L - 723.79 + 64.66 U/L (P<0.001): ASP. 0.63+0.20 U/L - 2.16 0.34 U/L (P<0.005), respectively. The results of metabolyres analysis of milk serum samples collected healthy and subclinical mastitis cows were as below; glucose, 3.52 € 0.42 mg/dl - 1.53 + 0.15 mg/dl (P<0.001): cholesterol, 7.27 0.76 mg/dl - 8.04 + 0.57 mg/dl (P>0.20): triglyceride. 19.33 + 1.77 mg/dl - 25.64+1.87 mg/dl (P>0.05), respectively. As a results, it was concluded that direct and indirect somatic cell counts of milk, determination of the level of Na, K, Cl, glucose and LDH, AST. ALT, CK, ALP, ASP activities in milk serum samples can be used satisfactory in the diagnosis of subclinical mastitis cases.
This study was conducted to investigate comparatively various biochemical test on normal and subclinical mastitic milk samples, diagnosed according to the California Mastitis Test (CMT), Direct Microscopic Somatic Cell Count (DMSCC). Differential Cell Count (DCC) results and to establish usefulness of these tests in the diagnosis of subclinical mastitis cases in cows. In the present, a total of 700 Holstein, Brown-Swiss cows, in 3-7 years old, supplied by Faculty of Veterinary Medicine University of Uludağ and commercial dairy farms in the region of research materials. CMT was performed previously on milk samples obtained from all cows. According to the test results. 30 mammary quarters shown" - " reactions were identified as free from subclinical mastitis. The other 70 mammary quarters reacted "?. I+, 2+. 3+" were identified as subclinical mastitis. Than, DMSCC and DCC were done. The results of mineral elements analysis of milk serum samples collected from healthy and subclinical mastitis cowe were shown as Na, % 43.37 = 2.92mg - % 58.51 3.68mg (P<0.005); K, % 97.53# 4.44mg - % 80.37 + 4.56mg (P<0.02): CI, % 72.95 + 3.26mg - % 108.27 = 4.50mg (P<0.001): Pi. % 2.34 + 0.41 mg - % 2.29 + 0.04mg (P>0.90). Mg. % 2.63 + 0.07mg - % 2.51 +0.04mg (P>0.20); Ca, % 40.36 = 2.29mg - % 48.05 + 1.85mg (P<0.05), respectively. The results of enzymes activity analysis of milk serum samples collected from healthy and subclinical mastitis cows were as belows; LDH, 39.83 = 10.98 U/L - 410.47 59.26 U/L (P<0.001), AST, 2.5 +0.77 U/L - 22.74 + 4.92 U/L (P<0.02), ALT: 4.5 +0.99 U/L. - 12.23 + 7.88 U/L (P<0.05); Amylase, 5.46 + 1.53 U/L - 6.67 + 1.86 U/L (P>0.50): GGT 7326.38 + 160.37 U/L - 1710.77 157.53 U/L (P>0.10): CK 4.86 + 0.68 U/L - 14.04 + 7.97 U/L (P<0.005): ALP. 261.47 + 34.25 U/L - 723.79 + 64.66 U/L (P<0.001): ASP. 0.63+0.20 U/L - 2.16 0.34 U/L (P<0.005), respectively. The results of metabolyres analysis of milk serum samples collected healthy and subclinical mastitis cows were as below; glucose, 3.52 € 0.42 mg/dl - 1.53 + 0.15 mg/dl (P<0.001): cholesterol, 7.27 0.76 mg/dl - 8.04 + 0.57 mg/dl (P>0.20): triglyceride. 19.33 + 1.77 mg/dl - 25.64+1.87 mg/dl (P>0.05), respectively. As a results, it was concluded that direct and indirect somatic cell counts of milk, determination of the level of Na, K, Cl, glucose and LDH, AST. ALT, CK, ALP, ASP activities in milk serum samples can be used satisfactory in the diagnosis of subclinical mastitis cases.
Description
Bu çalışma, Bursa Uludağ Üniversitesi Sosyal Bilimler Enstitüsünde E. Fatih Ünal'ın danışmanlığında Deniz Nak tarafından yazılan "Subklinik mastitislerin teşhis yöntemleri üzerine çalışmalar" adlı doktora tezine dayanılarak hazırlanmıştır.
Keywords
İnek, Biyokimyasal değişiklikler, Teşhis, Subklinik mastitis, Cow, Biochemical changes, Diagnosis, Subclinical mastitis
Citation
Nak, D. (1999). "Subklinik mastitislerin teşhis yöntemleri üzerine çalışmalar". Uludağ Üniversitesi Veteriner Fakültesi Dergisi, 18(3), 15-27.