Farklı dozlardaki gonadotropinlerin ovaryum follikül sayıları üzerine etkisi
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Date
2018-07-26
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Bursa Uludağ Üniversitesi
Abstract
Çalışmamızın amacı farelere, farklı dozlarda Pregnant Mare Serum Gonadotropin (PMSG) ve takiben Human Chorionic Gonadotropin (hCG) uygulamalarının, ovaryumda gelişmekte olan folikül ve korpus luteum sayılarına olan etkilerinin istatistiksel yöntemler ile belirlenmesidir. Çalışma materyali olarak 7 haftalık 40 adet BALC/c soyu dişi fare kullanıldı. Fareler, rastgele dört gruba ayrılarak, kontrol grubuna, 0,2 ml serum fizyolojik enjekte edildi. Diğer üç gruba subkutan yolla sırasıyla 2,5; 5 ve 7,5 I. U. PMSG hormonu verildi. PMSG enjeksiyonunun izleyen 48’inci saatte, deney gruplarına, PMSG’nin artan dozuna paralel olarak, gruplara sırasıyla 2,5; 5 ve 7,5 I. U. hCG, kontrol grubuna ise 0,2 ml serum fizyolojik subkutan yolla enjekte edildi. Anestezi altında alınan ve tespit edilen ovaryumlara rutin histolojik metodlar uygulanarak; ovaryum foliküllerinin ve korpus luteumların sayılması için Crossmon’ın üçlü boyama tekniği uygulandı. Çalışmamızda 3 farklı dozda yapmış olduğumuz uygulamalarda tüm deney gruplarında foliküler gelişimin kontrole oranla daha fazla olduğu görüldü. Gelişen folikül sayısının I. deney grubunda en çok olduğu sırasıyla III. ve II. deney grubu ile en az kontrol grubunda olduğu saptandı. Ovulasyon açısından korpus luteumlar değerlendirildiğinde en çok III. deney grubu sırasıyla II. ve I. deney grupları bulunurken en az kontrol grubunda olduğu görüldü. Uygulanan protokollerin hepsinde kullanılan gonadotropin dozuna bağlı olarak foliküler gelişimin ve ovulasyonun arttığı saptandı. Sonuç olarak; çalışmada en çok korpus luteum oluşumunun saptandığı III. deney grubunun (7,5 I.U. PMSG ve ardından 7,5 I.U. hCG’nin s.c.enjeksiyonunun) histolojik değerlendirmeler sonucunda, BALC/c soyu dişi fareler için en uygun süperovulasyon protokolü olduğuna karar verildi.
The aim of our study is to determine the effect of PMSG and following hCG administration to seven-week-old mice follicle development and corpus luteum formation by statistically methods. Seven-week-old, fourty BALB/c breed female mice were used in this study. Mice were divided in to four groups randomly, and 0,2 ml buffer saline was injected to control group. PMSG hormone was administrated at doses of 2,5; 5 and 7,5 I.U. respectively to other three groups. hCG was injected 2,5; 5 and 7,5 I.U. subcutaneous doses respectively to the experiment groups after 48 hours later than PMSG administration and 0,2 ml buffer saline was injected subcutaneously to control group. Ovaries were collected and fixed for histological examination and Crossmon’s triple staining method was applied for examination of follicle and corpus luteum developments in all experiment groups were more than control group. First experiment group had the most developing follicle number, followed by third and second experiment group respectively and at least control group. When corpus luteums were evaluated in terms of ovulation, it was seen that the third, second and first experiment groups were the most respectively and the control was the least. In all procedures, follicular development and ovulation were increased depending on the applied dose of gonadotropin. As a result of this study; it was decided, third experimental group had the most number of corpus luteum formation (7,5 I.U. PMSG and following 7,5 I.U. hCG subcutanous administration) was the most appropriate superovulation procedure for BALB/c breed female mice.
The aim of our study is to determine the effect of PMSG and following hCG administration to seven-week-old mice follicle development and corpus luteum formation by statistically methods. Seven-week-old, fourty BALB/c breed female mice were used in this study. Mice were divided in to four groups randomly, and 0,2 ml buffer saline was injected to control group. PMSG hormone was administrated at doses of 2,5; 5 and 7,5 I.U. respectively to other three groups. hCG was injected 2,5; 5 and 7,5 I.U. subcutaneous doses respectively to the experiment groups after 48 hours later than PMSG administration and 0,2 ml buffer saline was injected subcutaneously to control group. Ovaries were collected and fixed for histological examination and Crossmon’s triple staining method was applied for examination of follicle and corpus luteum developments in all experiment groups were more than control group. First experiment group had the most developing follicle number, followed by third and second experiment group respectively and at least control group. When corpus luteums were evaluated in terms of ovulation, it was seen that the third, second and first experiment groups were the most respectively and the control was the least. In all procedures, follicular development and ovulation were increased depending on the applied dose of gonadotropin. As a result of this study; it was decided, third experimental group had the most number of corpus luteum formation (7,5 I.U. PMSG and following 7,5 I.U. hCG subcutanous administration) was the most appropriate superovulation procedure for BALB/c breed female mice.
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Keywords
Süperovulasyon, Superovulation, hCG, Ovaryum, Fare, PMSG, Ovarium, Mouse
Citation
Pancaroğlu, G. vd. (2019). "Farklı dozlardaki gonadotropinlerin ovaryum follikül sayıları üzerine etkisi". Bursa Uludağ Üniversitesi Veteriner Fakültesi, 38(1), 39-45.