Publication: Arpa çeşitlerinin çimlenme döneminde ozmotik stres toleransının belirlenmesi
Date
2024-04-05
Authors
Authors
Dirik, Kübra Özdemir
Erdem, Mazlum
Saygılı, İbrahim
Journal Title
Journal ISSN
Volume Title
Publisher
Bursa Uludağ Üniversitesi
Abstract
Osmotik stres çimlenme döneminde kuraklık stresini stimüle ederek kuraklık toleransının ölçümünü sağlamaktadır. Bu araştırma ozmotik stres altında arpa çeşitlerinin çimlenme dönemindeki kuraklığa toleransının belirlenmesi amacıyla yürütülmüştür. Çalışmada 13’ü altı sıralı ve 23’ü iki sıralı olmak üzere 36 arpa çeşidi kullanılmıştır. Ozmotik stres polietilen glikol 6000 kullanılarak -3 bar ve -6 bar osmotik stres uygulanmış, stresin olmadığı uygulamada (kontrol) saf su kullanılmıştır. Araştırmanın yedinci gününde çimlenme oranı, kök uzunluğu ve sürgün uzunluğu belirlenmiştir. Çeşitlerin çimlenme oranları kontrole göre -3 bar ozmotik streste %5.7, -6 bar ozmotik streste ise %52.3 oranında önemli bir şekilde azalmıştır. Çeşitlerin kök uzunlukları -3 bar osmotik streste %14.4 artarken, -6 bar osmotik streste %56.1 azalmış, sürgün uzunlukları -3 bar osmotik streste %15.5 ve -6 bar osmotik streste %86.2 oranında azalmıştır. Genel ortalamada Kendal, Hasat, Dara, Yüksel, Etincel ve Epona çeşitlerinde en yüksek, Steptoe çeşidin de ise en düşük çimlenme oranı belirlenmiştir. Kök uzunluğu ve sürgün uzunluğu değerlerine göre Kral 97, Barış, Çetin 2000, Tarm 92, Sabribey ve Hasat çeşitlerinin osmotik strese daha toleranslı olduğu söylenebilir. Çeşitlerin kuraklık tolerans indeksleri incelenen üç özellikte de -6 bar osmotik streste -3 bar osmotik strese göre azalmıştır. -6 bar osmotik streste, en yüksek kuraklık tolerans indeksi, çimlenme oranında Kendal ve Dara (%86.7) çeşitlerinden, kök uzunluğunda Hasat (%98.1) çeşidinden, sürgün uzunluğundaEpona (%62.9) çeşidinden elde edilmiştir. Çimlenme döneminde kuraklık stresinin etkili olduğu bölgelerde, osmotik stres toleransı iyi olan çeşitlerin tarla denemelerine dahil edilmesi arpa üretimine katkı sağlayabilir.
Osmotic stress stimulates drought stress during germination and provides a measure of drought tolerance. This study was performed to determine the drought tolerance of barley cultivars under osmotic stress during germination. Thirty-six barley cultivars, 13 six-row and 23 two-row, were used. Osmotic stress was applied at -3 bar and -6 bar using polyethylene glycol 6000 and pure water was used in the non-stress treatment (control). Germination rate, root length and shoot length were determined on the seventh day of the experiment. Germination rates of the cultivars decreased by 5.7% at -3 bar osmotic stress and 52.3% at -6 bar osmotic stress compared to the control. Root length of the cultivars increased by 14.4% at -3 bar osmotic stress, decreased by 56.1% at -6 bar osmotic stress, and shoot length decreased by 15.5% at -3 bar osmotic stress and 86.2% at -6 bar osmotic stress. In the general average, the highest germination ratio was determined in cultivars Kendal, Hasat, Dara, Yüksel, Etincel and Epona and the lowest germination ratio was determined in Steptoe. According to root length and shoot length values, it can be stated that cultivars Kral 97, Barış, Çetin 2000, Tarm 92, Sabribey and Hasat are more tolerant to osmotic stress. Drought tolerance indexes of the cultivars decreased at -6 bar osmotic stress compared to -3 bar osmotic stress in all three traits examined. At -6 bar osmotic stress, the highest drought tolerance index was obtained from Kendal and Dara (86.7%) in germination rate, Hasat (98.1%) in root length and Epona (62.9%) in shoot length. In regions where drought stress is occurring during the germination period, inclusion of cultivars with better osmotic stress tolerance to field trials in these regions may contribute to barley production.
Osmotic stress stimulates drought stress during germination and provides a measure of drought tolerance. This study was performed to determine the drought tolerance of barley cultivars under osmotic stress during germination. Thirty-six barley cultivars, 13 six-row and 23 two-row, were used. Osmotic stress was applied at -3 bar and -6 bar using polyethylene glycol 6000 and pure water was used in the non-stress treatment (control). Germination rate, root length and shoot length were determined on the seventh day of the experiment. Germination rates of the cultivars decreased by 5.7% at -3 bar osmotic stress and 52.3% at -6 bar osmotic stress compared to the control. Root length of the cultivars increased by 14.4% at -3 bar osmotic stress, decreased by 56.1% at -6 bar osmotic stress, and shoot length decreased by 15.5% at -3 bar osmotic stress and 86.2% at -6 bar osmotic stress. In the general average, the highest germination ratio was determined in cultivars Kendal, Hasat, Dara, Yüksel, Etincel and Epona and the lowest germination ratio was determined in Steptoe. According to root length and shoot length values, it can be stated that cultivars Kral 97, Barış, Çetin 2000, Tarm 92, Sabribey and Hasat are more tolerant to osmotic stress. Drought tolerance indexes of the cultivars decreased at -6 bar osmotic stress compared to -3 bar osmotic stress in all three traits examined. At -6 bar osmotic stress, the highest drought tolerance index was obtained from Kendal and Dara (86.7%) in germination rate, Hasat (98.1%) in root length and Epona (62.9%) in shoot length. In regions where drought stress is occurring during the germination period, inclusion of cultivars with better osmotic stress tolerance to field trials in these regions may contribute to barley production.
Description
Keywords
Hordeum vulgare, Kök uzunluğu, Sürgün uzunluğu, Drought tolerance, PEG, Root length, Shoot length, Kuraklık toleransı PEG