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AĞCA, HARUN

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AĞCA

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HARUN

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2018 - 20192
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    Publication
    Viral and atypical bacterial respiratory infections in a university teaching hospital
    (Natl Inst Infectious Diseases, 2019-09-01) Harun, Ağca; Beyza, Ener; AĞCA, HARUN; ENER, BEYZA; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Mikrobiyoloji Anabilim Dalı; 0000-0002-2651-2034; AAH-4027-2021; ISU-9626-2023
    Respiratory viral and atypical bacterial agents lead to infections in a large spectrum, from mild symptoms to respiratory failure. In the present study, we aimed to detect multiple viral and bacterial agents in the respiratory samples of inpatients by real-time polymerase chain reaction (RT-PCR). Nasopharyngeal swabs and broncho-alveolar lavage samples from inpatients with respiratory infection symptoms at the Uludag University Hospital between December 1, 2015 and March 31,2018 were investigated. DNA/RNA was extracted using the EZ1 Virus Mini Kit v2.0 (Qiagen, Belgium) with the EZ1 extraction device (Qiagen, Belgium). The R-GENE (R) RT-PCR (Biomerioux, France) kit was used to detect influenza A, influenza B, respiratory syncytial virus (RSV), human metapneumovirus, rhinovirus/enterovirus (RV/EV), adenovirus, human bocavirus (hBoV), corona virus, parainfluenza virus, Chlamydia pneumoniae/Mycoplasma pneumoniae, and Legionella pneumophila in Rotor-Gene Q (Qiagen, Belgium). Patients were aged between 0 and 90 years. Overall, 177 (56.9%) patients were men and 134 (43.1%) were women. A total of 311 samples were analyzed, of which 214 (68.8%) were positive. In total, 360 agents, including 338 viruses and 22 bacteria, were detected. The commonest agents were influenza A+B (n = 65, 18,1%), hBoV (n = 64, 17.8%), RV/EV (n = 56, 15.6%), and RSV (n = 47, 13.1%). Rapid diagnosis of viral infections by RT-PCR is important for the specific treatment of patients.
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    Evaluation of 2015-2016 MOTAKK HBV DNA and HCV RNA external quality assessment national program results
    (Ankara Mikrobiyoloji Kulübü, 2018-10-01) Karataylı, Ersin; Soydemir, Ege; Aksoy, Zeynep Buşra; Kızılpınar, Mehtap; Altay Kocak, Aylin; Karataylı, Senem Ceren; Yurdcu, Esra; Yıldırım, Umut; Guriz, Haluk; Bozdayı, Gülendam; Yurdaydın, Cihan; İlhan, Osman; Yıldırım, Yasin; Bozdayı, A. Mithat; Oğuz, Acelya Yalçıntaş; Barış, Ahmet; Alp, Alpaslan; Aksözek, Alper; Sayıner, Arzu; Karagül, Aydan; Ordu, Aylin; İstanbullu, Aye; Otlu, Barış; Arıdoğan, Buket; Aksu, Burak; Buruk, C. Kurtuluş; Karahan, Ceren; Güney, Çakır; Toksöz, Devrim; Yıldırım, Dilara; Çolak, Dilek; Dağlar, Duygu Eren; Fındık, Duygu; Kas, Elif; Calışkan, Emel; Zeyrek, Fadile Yıldız; Arslan, Fatma; Demir, Feyza; Milletli, Fikriye; Kibar, Filiz; Özdinçer, Furkan; Dündar, Gülnür; Arslan, Hande; Ağça, Harun; Aliskan, Hikmet Eda; Güdücuoğlu, Hüseyin; Fidan, Işıl; Akyar, Isin; Afsar, Ilhan; Kaleli, İlknur; Dönmez, İsmail; YanIk, Kemalettin; Midilli, Kenan; çubukcu, Kivanc; Özdemir, Mehmet; Acar, Melek; Yalinay, Meltem; Kuşkucu, Mert Ahmet; Bakırcı, Mustafa Zahir; Aydın, Neriman; Yılmaz, Neziha; Çeken, Nihan; Ziyade, Nihan; Yılmaz, Nisel; Özgümüş, Osman Birol; Gitmişoğlu, Özlem; Demirgan, Recep; Kesli, Recep; Güçkan, Rıdvan; Sertoz, Ruchan; Akgün, Sadık; Aksaray, Sebahat; Tezcan, Seda; Kaygusuz, Sedat; Gökahmetoğlu, Selma; Meşe, Sevim; Bayik, Seyit Ahmet; Akcalı, Sinem; Gürcan, Saban; Karsligil, Tekin; Us, Tercan; Özekinci, Tuncer; Pilgir, Tulin; Aslan, Uğur; Dinc, Ugur; Coşkun, Umut Safiye Say; Çetinkol, Yeliz; Keskin, Yusuf; Ayaydın, Zeynep; Toraman, Zulal Asçı; MOTAKK HBV HCV Calisma Grubu; AĞCA, HARUN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji Anabilim Dalı; 0000-0002-2651-2034; ISU-9626-2023
    MOTAKK, as a national external quality control program has been launched to evaluate the molecular detection of viral infections including HBV DNA and HCV RNA in molecular microbiology diagnostic laboratories in Turkey. This program is prepared in compliance with ISO 17043:2010 (Conformity assessment general requirements for proficiency testing) standards, and aims to take the place of external quality control programs from abroad, contributing to standardization and accuracy of molecular diagnostic tests in our country. The aim of this study was to evaluate 2015 and 2016 results of the MOTAKK External Quality Control Program for HBV DNA and HCV RNA viral load. The calls were announced on the web page of MOTAKK (www.motakk.org). The quality control samples were sent to participating laboratories in 2015 and 2016. Main stocks were prepared from patients with chronic hepatitis B and C who had viral load detection with reference methods according to WHO reference materials for viral load studies to improve quality control sera. From these main stocks, samples with different viral loads were prepared from dilutions of plasma with HBV, HCV, HAV, HIV, Parvovirus B19 and CMV negative serologic markers. Quality control samples were sent to the participating laboratories along with the negative samples in the cold chain. The laboratories accomplished the related tests within 2-3 weeks and entered their results on the MOTAKK web page. These results were analysed according to ISO 13528 (Statistical methods for use in proficiency testing by interlaboratory comparison) and scoring reports were created by a software developed by MOTAKK and sent to participating labs. Each laboratory evaluated their own results in comparison with the other laboratory results, reassessed the tests via observing the distance from the mean result and the reference values. The number of laboratories participating in the HBV DNA and HCV RNA external quality control program was 70-73 in 2015-2016. Participants were able to comply with the program tools, registering, entering results and receiving the results reports problem. In HBV panel, 72.6-89.1% and 84.7-90.3% of the participant laboratories were in 1 standard deviation (SD) in 2015-2016, respectively. In HCV panel, 70.8-89.1% and 84.7-90.3% of the participant laboratories were in 1 SD in 2015-2016, respectively. A national external quality control program for HBV DNA and HCV RNA in Turkey has been prepared for the first time with this project and implemented successfully. All the data provided in the MOTAKK external quality control program final report, compensate all the data provided by the quality control program final reports from abroad; additionally, the report allows comparison of used technologies and commercial products.